Nuclear Receptor ROR-Gamma (RORC) ELISA Kit for Human

Background: Nuclear Receptor ROR-gamma (RORC) is a transcription factor belonging to the retinoic acid receptor-related orphan receptor (ROR) family. It is predominantly expressed in immune cells, where it regulates the differentiation and function of T helper 17 (Th17) cells, a subset of T lymphocytes involved in host defense and autoimmune responses. Nuclear Receptor ROR-gamma controls the expression of genes encoding cytokines such as interleukin-17 (IL-17) and interleukin-22 (IL-22), thereby influencing immune homeostasis and inflammatory processes. Dysregulation of Nuclear Receptor ROR-gamma activity is implicated in autoimmune diseases, inflammatory disorders, and metabolic conditions.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti RORC antibody was precoated onto the 96-well plate. The biotin conjugated anti RORC antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with RORC conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of RORC in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

Small volumes of RORC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS760662 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nuclear receptor ROR-gamma (RORC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing RORC. The ELISA analytical biochemical technique of the MBS760662 kit is based on RORC antibody-RORC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect RORC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, RORC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).