Cytochrome C Oxidase Subunit 5a, Mitochondrial (COX5A) ELISA Kit for Human

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human COX5A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human COX5A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human COX5A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human COX5A can be calculated.

Small volumes of COX5A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS760845 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytochrome c oxidase subunit 5A, mitochondrial (COX5A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing COX5A. The ELISA analytical biochemical technique of the MBS760845 kit is based on COX5A antibody-COX5A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect COX5A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, COX5A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).