Protein KIBRA (WWC1) ELISA Kit for Human

Name: Human WWC1 ELISA Kit; Protein KIBRA; MyBioSource
Brand: MyBioSource
SKU: MBS760854
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS760854

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- WWC1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- WWC1 antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Protein KIBRA (WWC1), ELISA Kit

Full Product Name

Human Protein KIBRA ELISA Kit

Product Gene Name

WWC1 elisa kit

Product Synonym Names

WWC1/KIAA0869/KIBRA/WW domain-containing protein 1/HBeAg-binding protein 3/Kidney and brain protein

Species Reactivity

Human

Assay Type

Sandwich ELISA, Double Antibody

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

78.125-5000pg/ml

Sensitivity

46.875pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of WWC1. No significant cross-reactivity or interference between WWC1 and analogues was observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WWC1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WWC1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%

Molecular Weight

125,905 Da

OMIM

610533

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of WWC1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WWC1 purchase

MBS760854 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein KIBRA (WWC1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WWC1. The ELISA analytical biochemical technique of the MBS760854 kit is based on WWC1 antibody-WWC1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WWC1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WWC1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for WWC1. It may not necessarily be applicable to this product.

NCBI GI #

242247257

NCBI GeneID

23286

NCBI Accession #

NP_001155134.1

UniProt Secondary Accession #

O94946; Q6MZX4; Q6Y2F8; Q7Z4G8; Q8WVM4; Q9BT29; B4DK05

UniProt Related Accession #

Q8IX03

GenBank Nucleotide #

NM_001161662.1

NCBI Official Full Name

protein KIBRA isoform 2

NCBI Official Synonym Full Names

WW and C2 domain containing 1

NCBI Official Symbol

WWC1

NCBI Official Synonym Symbols

KIBRA; HBEBP3; HBEBP36; MEMRYQTL; PPP1R168

UniProt Protein Name

Protein KIBRA

UniProt Synonym Protein Names

HBeAg-binding protein 3; Kidney and brain protein; KIBRA; WW domain-containing protein 1

Protein Family

Protein

UniProt Gene Name

WWC1

UniProt Synonym Gene Names

KIAA0869; KIBRA

UniProt Entry Name

KIBRA_HUMAN

NCBI Protein Information

protein KIBRA

NCBI Summary for WWC1

The protein encoded by this gene is a cytoplasmic phosphoprotein that interacts with PRKC-zeta and dynein light chain-1. Alleles of this gene have been found that enhance memory in some individuals. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

UniProt Comments for WWC1

KIBRA: a postsynaptic scaffolding protein associated with human memory performance. Interacts via its WW domains with the postsynaptic protein DDN, which plays a role in synaptic plasticity. Expressed in the hypothalamus and cerebellum, and in the hippocampus and cortex, both regions of the brain that are related to memory. Co-localizes with PKCZ in the mouse hippocampus. Binds to synaptopodin, modulating podocyte directional migration. Forms a complex with dynein light chain 1 that is recruited to ER-responsive promoters, and that is required for ER transactivation in breast cancer cells. Binds the minus end-directed microtubule motor dynein. Its amino terminus binds the eight PDZ domain of the polarity protein INADL . Two human isoforms have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: cytoplasm; cytosol; nucleus; perinuclear region of cytoplasm; protein complex

Molecular Function: kinase binding; protein binding; protein binding, bridging; protein complex scaffold; transcription coactivator activity

Biological Process: cell migration; establishment of cell polarity; negative regulation of organ growth; negative regulation of transcription from RNA polymerase II promoter; positive regulation of MAPKKK cascade; regulation of intracellular transport; transcription, DNA-dependent

Disease: Memory Quantitative Trait Locus

Research Articles on WWC1

1. the study demonstrates an association between the KIBRA gene and episodic memory (immediate free recall) and suggests a differential effect of this genetic variant in early-onset schizophrenia and healthy siblings

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with WWC1 elisa kit

Reference Product	PubMed Publications
LATS1 elisa kit	>4 publications with WWC1 and LATS1
LATS2 elisa kit	>3 publications with WWC1 and LATS2
NF2 elisa kit	>2 publications with WWC1 and NF2
FRMD1 elisa kit	>1 publications with WWC1 and FRMD1
CDC14A elisa kit	>1 publications with WWC1 and CDC14A
PTPN14 elisa kit	>1 publications with WWC1 and PTPN14
SNX4 elisa kit	>1 publications with WWC1 and SNX4

Pathways associated with WWC1 elisa kit

Products by Pathway	Pathway Diagram
Hippo Signaling Pathway antibodies	Hippo Signaling Pathway Diagram
Hippo Signaling Pathway antibodies	Hippo Signaling Pathway Diagram
Hippo Signaling Pathway antibodies	Hippo Signaling Pathway Diagram
Hippo Signaling Pathway antibodies	Hippo Signaling Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By Hippo Pathway antibodies	Signaling By Hippo Pathway Diagram

Diseases associated with WWC1 elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>14 publications with WWC1 and Nervous System Diseases
Learning Disorders Antibodies	>4 publications with WWC1 and Learning Disorders
Hypertension Antibodies	>4 publications with WWC1 and Hypertension
Breast Neoplasms Antibodies	>3 publications with WWC1 and Breast Neoplasms
Kidney Diseases Antibodies	>2 publications with WWC1 and Kidney Diseases
Adenocarcinoma Antibodies	>1 publications with WWC1 and Adenocarcinoma
Cardiovascular Diseases Antibodies	>1 publications with WWC1 and Cardiovascular Diseases
Neoplasm Metastasis Antibodies	>1 publications with WWC1 and Neoplasm Metastasis
Ovarian Neoplasms Antibodies	>1 publications with WWC1 and Ovarian Neoplasms
Carcinoma, Hepatocellular Antibodies	>1 publications with WWC1 and Carcinoma, Hepatocellular

Organs/Tissues associated with WWC1 elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>31 publications with WWC1 and Brain
Kidney Antibodies	>13 publications with WWC1 and Kidney
Nerve Antibodies	>3 publications with WWC1 and Nerve
Muscle Antibodies	>2 publications with WWC1 and Muscle
Lung Antibodies	>2 publications with WWC1 and Lung
Trachea Antibodies	>1 publications with WWC1 and Trachea
Pancreas Antibodies	>1 publications with WWC1 and Pancreas
Stomach Antibodies	>1 publications with WWC1 and Stomach
Mammary Gland Antibodies	>1 publications with WWC1 and Mammary Gland