Pancreatic Prohormone (PPY) ELISA Kit for Human

Name: Human PPY ELISA Kit; Pancreatic Prohormone; MyBioSource
Brand: MyBioSource
SKU: MBS760864
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS760864

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with PGF2alpha. During the reaction, PGF2alpha in the sample or standard competes with a fixed amount of PGF2alpha… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Pancreatic prohormone (PPY), ELISA Kit

Full Product Name

Human Pancreatic prohormone ELISA Kit

Product Gene Name

PPY elisa kit

Product Synonym Names

PPY/INN/Obinepitide/PNP/PP/Obinepitide/pancreatic polypeptide (ppy)/pancreatic polypeptide/pancreatic polypeptide Y/pancreatic prohormone

Species Reactivity

Human

Assay Type

Competitive ELISA, Coated with Antigen

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

46.875-3000pg/ml

Sensitivity

28.125pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of PGF2alpha. No significant cross-reactivity or interference between PGF2alpha and analogues is observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PGF2alpha were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PGF2alpha were tested 20 times on one plate, respectively. Intra-Assay: CV<8%

Molecular Weight

18,786 Da

OMIM

167780

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of PPY elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PPY purchase

MBS760864 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Pancreatic prohormone (PPY) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PPY. The ELISA analytical biochemical technique of the MBS760864 kit is based on PPY antibody-PPY antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PPY antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PPY. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PPY. It may not necessarily be applicable to this product.

NCBI GI #

4506033

NCBI GeneID

5539

NCBI Accession #

NP_002713.1

UniProt Related Accession #

P01298

GenBank Nucleotide #

NM_002722.4

NCBI Official Full Name

pancreatic prohormone isoform 1 preproprotein

NCBI Official Synonym Full Names

pancreatic polypeptide

NCBI Official Symbol

PPY

NCBI Official Synonym Symbols

PP; PNP

UniProt Protein Name

Pancreatic prohormone

UniProt Synonym Protein Names

Pancreatic polypeptide; PP

Protein Family

Pancreatic hormone

UniProt Gene Name

PPY

UniProt Synonym Gene Names

PNP; PP; PH; PI

UniProt Entry Name

PAHO_HUMAN

NCBI Protein Information

pancreatic prohormone

NCBI Summary for PPY

This gene encodes a member of the neuropeptide Y (NPY) family of peptides. The encoded 95 aa preproprotein is synthesized in the pancreatic islets of Langerhans and proteolytically processed to generate two peptide products. These products include the active pancreatic hormone of 36 aa and an icosapeptide of unknown function. This hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]

UniProt Comments for PPY

PPY: Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions. Belongs to the NPY family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytoplasm; extracellular region; extracellular space

Molecular Function: G-protein-coupled receptor binding; hormone activity; neuropeptide hormone activity; receptor binding

Biological Process: cell-cell signaling; digestion; feeding behavior; neuropeptide signaling pathway; positive regulation of defense response to virus by host; protein secretion; regulation of appetite

Research Articles on PPY

1. These data demonstrate glucose-regulated secretion of PP and its effects on glucagon release through PPYR1 receptors expressed by alpha-cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PPY elisa kit

Reference Product	PubMed Publications
SST elisa kit	>5 publications with PPY and SST
GHRL elisa kit	>3 publications with PPY and GHRL
NPY2R elisa kit	>2 publications with PPY and NPY2R
NPY5R elisa kit	>1 publications with PPY and NPY5R
GAL elisa kit	>1 publications with PPY and GAL
NPY1R elisa kit	>1 publications with PPY and NPY1R

Pathways associated with PPY elisa kit

Products by Pathway	Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
G Alpha (i) Signalling Events Pathway antibodies	G Alpha (i) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
Peptide Ligand-binding Receptors Pathway antibodies	Peptide Ligand-binding Receptors Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By GPCR Pathway antibodies	Signaling By GPCR Pathway Diagram

Diseases associated with PPY elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>13 publications with PPY and Cardiovascular Diseases
Inflammation Antibodies	>13 publications with PPY and Inflammation
Necrosis Antibodies	>12 publications with PPY and Necrosis
Diabetes Mellitus, Type 2 Antibodies	>7 publications with PPY and Diabetes Mellitus, Type 2
Disease Models, Animal Antibodies	>7 publications with PPY and Disease Models, Animal
Neoplasms, Experimental Antibodies	>5 publications with PPY and Neoplasms, Experimental
Liver Diseases Antibodies	>4 publications with PPY and Liver Diseases
Diabetes Mellitus, Type 1 Antibodies	>3 publications with PPY and Diabetes Mellitus, Type 1
Hemorrhage Antibodies	>3 publications with PPY and Hemorrhage
Hyperplasia Antibodies	>3 publications with PPY and Hyperplasia

Organs/Tissues associated with PPY elisa kit

Organ/Tissue Name	Pubmed Publications
Pancreas Antibodies	>15 publications with PPY and Pancreas
Connective Tissue Antibodies	>12 publications with PPY and Connective Tissue