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Aldehyde Oxidase (AOX1) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS760905

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Aox1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Aox1 antibody was used as detection antibodies. The standards,… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Aldehyde oxidase (Aox1), ELISA Kit
Full Product Name
Rat Aldehyde oxidase ELISA Kit
Product Gene Name
Aox1 elisa kit
Product Synonym Names
Aox1/AOX1/aldehyde oxidase/aldehyde oxidase 1/AOAOH1/EC 1.2.3.1
Species Reactivity
Rat
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.781-50ng/ml
Sensitivity
0.469ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
147,918 Da
OMIM
602841
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of Aox1 elisa kit

Rat AOX1 ELISA Kit – Cat# MBS760905

Typical Standard Curve of Aox1 elisa kit

Rat AOX1 ELISA Kit, Typical Standard Curve – Cat# MBS760905

Other Notes

Small volumes of Aox1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Aox1 purchase

MBS760905 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Aldehyde oxidase (Aox1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Aox1. The ELISA analytical biochemical technique of the MBS760905 kit is based on Aox1 antibody-Aox1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Aox1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Aox1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Aox1. It may not necessarily be applicable to this product.

NCBI GI #
71773480
NCBI GeneID
316
NCBI Accession #
NP_001150.3
UniProt Secondary Accession #
O14765; Q53RR8; Q53TV3; Q9BYF0; Q9UPG6
UniProt Related Accession #
Q06278
GenBank Nucleotide #
NM_001159.3
NCBI Official Full Name
aldehyde oxidase
NCBI Official Synonym Full Names
aldehyde oxidase 1
NCBI Official Symbol
AOX1
NCBI Official Synonym Symbols
AO; AOH1
UniProt Protein Name
Aldehyde oxidase
UniProt Synonym Protein Names
Aldehyde oxidase 1; Azaheterocycle hydroxylase (EC:1.17.3.-)
Protein Family
Alcohol oxidase
UniProt Gene Name
AOX1
UniProt Synonym Gene Names
AO
UniProt Entry Name
AOXA_HUMAN
NCBI Protein Information
aldehyde oxidase

NCBI Summary for Aox1

Aldehyde oxidase produces hydrogen peroxide and, under certain conditions, can catalyze the formation of superoxide. Aldehyde oxidase is a candidate gene for amyotrophic lateral sclerosis. [provided by RefSeq, Jul 2008]

UniProt Comments for Aox1

AOX1: Aldehyde oxidase produces hydrogen peroxide and, under certain conditions, can catalyze the formation of superoxide. Aldehyde oxidase is a candidate gene for amyotrophic lateral sclerosis. [provided by RefSeq, Jul 2008]

Protein type: Oxidoreductase; EC 1.2.3.1; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Cofactor and Vitamin Metabolism - vitamin B6

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: cytoplasm; cytosol

Molecular Function: 2 iron, 2 sulfur cluster binding; aldehyde oxidase activity; electron carrier activity; FAD binding; iron ion binding; molybdopterin cofactor binding; NAD binding; oxidoreductase activity, acting on CH-OH group of donors; xanthine dehydrogenase activity

Biological Process: inflammatory response; vitamin B6 metabolic process; vitamin metabolic process; water-soluble vitamin metabolic process; xanthine catabolic process
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Research Articles on Aox1

1. Data suggest that substrate specificity of AOX1 includes GDC-0834, an inhibitor of Bruton's tyrosine kinase with potential non-steroidal anti-inflammatory agent activity; in liver cytosol, hydrolysis of GDC-0834 is primarily due to AOX1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Aox1 elisa kit

 Reference Product  PubMed Publications
 XDH elisa kit  >3 publications with Aox1 and XDH
 MOCOS elisa kit  >2 publications with Aox1 and MOCOS
 NNMT elisa kit  >1 publications with Aox1 and NNMT

Pathways associated with Aox1 elisa kit

 Products by Pathway  Pathway Diagram
 Drug Metabolism - Cytochrome P450 Pathway antibodies  Drug Metabolism - Cytochrome P450 Pathway Diagram
 Drug Metabolism - Cytochrome P450 Pathway antibodies  Drug Metabolism - Cytochrome P450 Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Jak-STAT Signaling Pathway antibodies  Jak-STAT Signaling Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Vitamins And Cofactors Pathway antibodies  Metabolism Of Vitamins And Cofactors Pathway Diagram
 Metabolism Of Water-soluble Vitamins And Cofactors Pathway antibodies  Metabolism Of Water-soluble Vitamins And Cofactors Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram
 Nicotinate And Nicotinamide Metabolism Pathway antibodies  Nicotinate And Nicotinamide Metabolism Pathway Diagram

Diseases associated with Aox1 elisa kit

 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >5 publications with Aox1 and Fatty Liver
 Liver Diseases Antibodies  >5 publications with Aox1 and Liver Diseases
 Prostatic Neoplasms Antibodies  >5 publications with Aox1 and Prostatic Neoplasms
 Cardiovascular Diseases Antibodies  >4 publications with Aox1 and Cardiovascular Diseases
 Neoplasms, Experimental Antibodies  >4 publications with Aox1 and Neoplasms, Experimental
 Weight Loss Antibodies  >3 publications with Aox1 and Weight Loss
 Liver Neoplasms Antibodies  >3 publications with Aox1 and Liver Neoplasms
 Disease Models, Animal Antibodies  >3 publications with Aox1 and Disease Models, Animal
 Adenocarcinoma Antibodies  >3 publications with Aox1 and Adenocarcinoma
 Carcinoma, Hepatocellular Antibodies  >2 publications with Aox1 and Carcinoma, Hepatocellular

Organs/Tissues associated with Aox1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >28 publications with Aox1 and Liver
 Vascular Antibodies  >12 publications with Aox1 and Vascular
 Prostate Antibodies  >5 publications with Aox1 and Prostate
 Skin Antibodies  >4 publications with Aox1 and Skin
 Brain Antibodies  >3 publications with Aox1 and Brain
 Muscle Antibodies  >3 publications with Aox1 and Muscle
 Intestine Antibodies  >3 publications with Aox1 and Intestine
 Kidney Antibodies  >3 publications with Aox1 and Kidney
 Bone Antibodies  >2 publications with Aox1 and Bone
 Heart Antibodies  >2 publications with Aox1 and Heart
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