Nuclear Factor Kappa B P65 (NF-kappaB p65) ELISA Kit for Human

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human NF-kappaB p65 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human NF-kappaB p65 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NF-kappaB p65 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human NF-kappaB p65 can be calculated.

Small volumes of NF-kappaB p65 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS761087 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nuclear Factor Kappa B p65 (NF-kappaB p65) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NF-kappaB p65. The ELISA analytical biochemical technique of the MBS761087 kit is based on NF-kappaB p65 antibody-NF-kappaB p65 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NF-kappaB p65 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NF-kappaB p65. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).