Amphiregulin (AR) ELISA Kit for Porcine

Name: Porcine AR ELISA Kit; Amphiregulin; MyBioSource
Brand: MyBioSource
SKU: MBS761145
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS761145

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Amphiregulin (AR), ELISA Kit

Full Product Name

Porcine Amphiregulin ELISA Kit

Product Gene Name

AR elisa kit

Species Reactivity

Porcine

Assay Type

Sandwich ELISA, Double Antibody

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

62.5-4000pg/ml

Sensitivity

37.5pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of Agt. No significant cross-reactivity or interference between Agt and analogues was observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

CV<10%

Intra-assay Precision

CV<8%

Molecular Weight

27,895 Da

OMIM

104640

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of AR elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AR purchase

MBS761145 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Amphiregulin (AR) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AR. The ELISA analytical biochemical technique of the MBS761145 kit is based on AR antibody-AR antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AR antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AR. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for AR. It may not necessarily be applicable to this product.

NCBI GI #

4502199

NCBI GeneID

374

NCBI Accession #

NP_001648.1

UniProt Secondary Accession #

Q5U026

UniProt Related Accession #

P15514

GenBank Nucleotide #

NM_001657.3

NCBI Official Full Name

amphiregulin preproprotein

NCBI Official Synonym Full Names

amphiregulin

NCBI Official Symbol

AREG

NCBI Official Synonym Symbols

AR; SDGF; AREGB; CRDGF

UniProt Protein Name

Amphiregulin

UniProt Synonym Protein Names

Colorectum cell-derived growth factor

Protein Family

Allatostatin

UniProt Gene Name

AREG

UniProt Synonym Gene Names

CRDGF

UniProt Entry Name

AREG_HUMAN

NCBI Protein Information

amphiregulin

NCBI Summary for AR

The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014]

UniProt Comments for AR

AREG: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. Belongs to the amphiregulin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: cell surface; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; extracellular space; Golgi membrane; integral to membrane; nucleus

Molecular Function: cytokine activity; epidermal growth factor receptor binding; growth factor activity; protein binding

Biological Process: cell proliferation; cell-cell signaling; cellular protein metabolic process; COPII coating of Golgi vesicle; epidermal growth factor receptor signaling pathway; ER to Golgi vesicle-mediated transport; G-protein coupled receptor protein signaling pathway; glial cell proliferation; negative regulation of osteoblast differentiation; neurite development; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of phosphorylation; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; response to cAMP; response to estradiol stimulus; response to glucocorticoid stimulus; response to hydrogen peroxide; response to peptide hormone stimulus

Research Articles on AR

1. AR induces hHSC fibrogenic activity via multiple mitogenic signaling pathways, and is upregulated in murine and human NASH, suggesting that AR antagonists may be clinically useful anti-fibrotics in NAFLD.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with AR elisa kit

Reference Product	PubMed Publications
EGFR elisa kit	>303 publications with AR and EGFR
CCND3 elisa kit	>2 publications with AR and CCND3
S100A4 elisa kit	>1 publications with AR and S100A4
S100A2 elisa kit	>1 publications with AR and S100A2
S100A5 elisa kit	>1 publications with AR and S100A5

Pathways associated with AR elisa kit

Products by Pathway	Pathway Diagram
Asparagine N-linked Glycosylation Pathway antibodies	Asparagine N-linked Glycosylation Pathway Diagram
COPII (Coat Protein 2) Mediated Vesicle Transport Pathway antibodies	COPII (Coat Protein 2) Mediated Vesicle Transport Pathway Diagram
Cargo Concentration In The ER Pathway antibodies	Cargo Concentration In The ER Pathway Diagram
ER To Golgi Anterograde Transport Pathway antibodies	ER To Golgi Anterograde Transport Pathway Diagram
ErbB Receptor Signaling Network Pathway antibodies	ErbB Receptor Signaling Network Pathway Diagram
ErbB Signaling Pathway antibodies	ErbB Signaling Pathway Diagram
ErbB Signaling Pathway antibodies	ErbB Signaling Pathway Diagram
ErbB Signaling Pathway antibodies	ErbB Signaling Pathway Diagram
FSH Signaling Pathway antibodies	FSH Signaling Pathway Diagram
Hippo Signaling Pathway antibodies	Hippo Signaling Pathway Diagram

Diseases associated with AR elisa kit

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>91 publications with AR and Breast Neoplasms
Necrosis Antibodies	>62 publications with AR and Necrosis
Inflammation Antibodies	>60 publications with AR and Inflammation
Lung Diseases Antibodies	>56 publications with AR and Lung Diseases
Adenocarcinoma Antibodies	>51 publications with AR and Adenocarcinoma
Neoplasms, Experimental Antibodies	>40 publications with AR and Neoplasms, Experimental
Lung Neoplasms Antibodies	>39 publications with AR and Lung Neoplasms
Liver Diseases Antibodies	>36 publications with AR and Liver Diseases
Cell Transformation, Neoplastic Antibodies	>28 publications with AR and Cell Transformation, Neoplastic
Hyperplasia Antibodies	>28 publications with AR and Hyperplasia

Organs/Tissues associated with AR elisa kit

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>92 publications with AR and Lung
Blood Antibodies	>74 publications with AR and Blood
Skin Antibodies	>55 publications with AR and Skin
Liver Antibodies	>50 publications with AR and Liver
Mammary Gland Antibodies	>43 publications with AR and Mammary Gland
Vascular Antibodies	>39 publications with AR and Vascular
Intestine Antibodies	>31 publications with AR and Intestine
Uterus Antibodies	>25 publications with AR and Uterus
Stomach Antibodies	>25 publications with AR and Stomach
Prostate Antibodies	>21 publications with AR and Prostate