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Calsequestrin-1 (CASQ1) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS761166

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Casq1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Casq1 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Calsequestrin-1 (Casq1), ELISA Kit
Full Product Name
Rat Calsequestrin-1 ELISA Kit
Product Gene Name
Casq1 elisa kit
Product Synonym Names
Casq1/Calsequestrin-1/Laminin-binding protein/Aspartactin/Calsequestrin; skeletal muscle isoform
Species Reactivity
Rat
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
78.125-5000pg/ml
Sensitivity
46.875pg/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
46,449 Da
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of Casq1 elisa kit

Rat CASQ1 ELISA Kit – Cat# MBS761166

Typical Standard Curve of Casq1 elisa kit

Rat CASQ1 ELISA Kit, Typical Standard Curve – Cat# MBS761166

Other Notes

Small volumes of Casq1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Casq1 purchase

MBS761166 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Calsequestrin-1 (Casq1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Casq1. The ELISA analytical biochemical technique of the MBS761166 kit is based on Casq1 antibody-Casq1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Casq1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Casq1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Casq1. It may not necessarily be applicable to this product.

NCBI GI #
227430324
NCBI GeneID
686019
NCBI Accession #
NP_001153066.1
UniProt Related Accession #
P19633
GenBank Nucleotide #
NM_001159594.1
NCBI Official Full Name
calsequestrin-1
NCBI Official Synonym Full Names
calsequestrin 1
NCBI Official Symbol
Casq1
UniProt Protein Name
Calsequestrin-1
UniProt Synonym Protein Names
Aspartactin; Calsequestrin, skeletal muscle isoform; Laminin-binding protein
Protein Family
Calsequestrin
UniProt Gene Name
Casq1
UniProt Entry Name
CASQ1_RAT
NCBI Protein Information
calsequestrin-1

UniProt Comments for Casq1

calsequestrin 1: Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. The skeletal muscle isoform (CASQ1) binds around 80 Ca(2+) ions, while the cardiac isoform (CASQ2) binds approximately 60 Ca(2+). Belongs to the calsequestrin family.

Protein type: Mitochondrial; Calcium-binding

Cellular Component: cytoplasm; Golgi apparatus; I band; intracellular membrane-bound organelle; mitochondrial matrix; mitochondrion; myofibril; sarcolemma; sarcoplasmic reticulum; sarcoplasmic reticulum lumen; sarcoplasmic reticulum membrane; T-tubule

Molecular Function: calcium ion binding

Biological Process: endoplasmic reticulum organization and biogenesis; protein polymerization; regulation of sequestering of calcium ion; regulation of skeletal muscle contraction via regulation of the release of sequestered calcium ion; response to denervation involved in regulation of muscle adaptation; response to heat; response to muscle inactivity; response to organic substance; sarcomere organization; skeletal muscle development
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Research Articles on Casq1

1. In EDL muscle, CSQ1 provides a large total pool of releasable Ca2+ in the SR whilst maintaining free [Ca2+] in the SR at sufficiently low levels that Ca2+ leakage through the high density of SERCA1 pumps does not metabolically compromise muscle function.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Casq1 elisa kit

 Reference Product  PubMed Publications
 Ryr1 elisa kit  >17 publications with Casq1 and Ryr1
 Atp2a1 elisa kit  >2 publications with Casq1 and Atp2a1
 Trdn elisa kit  >2 publications with Casq1 and Trdn
 Tmod4 elisa kit  >1 publications with Casq1 and Tmod4
 Asph elisa kit  >1 publications with Casq1 and Asph

Pathways associated with Casq1 elisa kit

 Products by Pathway  Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram

Diseases associated with Casq1 elisa kit

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >3 publications with Casq1 and Inflammation
 Necrosis Antibodies  >3 publications with Casq1 and Necrosis
 Atrophy Antibodies  >2 publications with Casq1 and Atrophy
 Myocardial Infarction Antibodies  >2 publications with Casq1 and Myocardial Infarction
 Congenital Abnormalities Antibodies  >1 publications with Casq1 and Congenital Abnormalities
 Nervous System Malformations Antibodies  >1 publications with Casq1 and Nervous System Malformations
 Lung Neoplasms Antibodies  >1 publications with Casq1 and Lung Neoplasms
 Dyslipidemias Antibodies  >1 publications with Casq1 and Dyslipidemias

Organs/Tissues associated with Casq1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >72 publications with Casq1 and Muscle
 Heart Antibodies  >13 publications with Casq1 and Heart
 Eye Antibodies  >6 publications with Casq1 and Eye
 Brain Antibodies  >3 publications with Casq1 and Brain
 Prostate Antibodies  >1 publications with Casq1 and Prostate
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