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Keratin, Type 1 Cytoskeletal 16 (KRT16) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS761367

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human KRT16 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human KRT16 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Keratin, type I cytoskeletal 16 (KRT16), ELISA Kit
Full Product Name
Human Keratin, type I cytoskeletal 16 ELISA Kit
Product Gene Name
KRT16 elisa kit
Product Synonym Names
KRT16/Cytokeratin-16 (CK-16)/Keratin-16/CK16/K1CP/NEPPK/FNEPPK/KRT16A
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
51,268 Da
OMIM
144200
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of KRT16 elisa kit

Human KRT16 ELISA Kit – Cat# MBS761367

Typical Standard Curve of KRT16 elisa kit

Human KRT16 ELISA Kit, Typical Standard Curve – Cat# MBS761367

Other Notes

Small volumes of KRT16 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT16 purchase

MBS761367 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Keratin, type I cytoskeletal 16 (KRT16) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KRT16. The ELISA analytical biochemical technique of the MBS761367 kit is based on KRT16 antibody-KRT16 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KRT16 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KRT16. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for KRT16. It may not necessarily be applicable to this product.

NCBI GI #
24430192
NCBI GeneID
3868
NCBI Accession #
NP_005548.2
UniProt Secondary Accession #
P30654; Q16402; Q9UBG8; A8K488
UniProt Related Accession #
P08779
GenBank Nucleotide #
NM_005557.3
NCBI Official Full Name
keratin, type I cytoskeletal 16
NCBI Official Synonym Full Names
keratin 16, type I
NCBI Official Symbol
KRT16
NCBI Official Synonym Symbols
K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A
UniProt Protein Name
Keratin, type I cytoskeletal 16
UniProt Synonym Protein Names
Cytokeratin-16; CK-16; Keratin-16; K16
Protein Family
Keratin
UniProt Gene Name
KRT16
UniProt Synonym Gene Names
KRT16A; CK-16; K16
UniProt Entry Name
K1C16_HUMAN
NCBI Protein Information
keratin, type I cytoskeletal 16

NCBI Summary for KRT16

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT16

K16: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K6. K16 and K17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoskeleton; intermediate filament; nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: aging; cell proliferation; cytoskeleton organization and biogenesis; epidermis development; hair cycle; inflammatory response; innate immune response; intermediate filament cytoskeleton organization and biogenesis; keratinization; keratinocyte differentiation; keratinocyte migration; morphogenesis of an epithelium; negative regulation of cell migration

Disease: Pachyonychia Congenita 1; Palmoplantar Keratoderma, Nonepidermolytic, Focal
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Research Articles on KRT16

1. The wider spectrum of KRT16 mutations suggests that changes in codons 125, 127, and 132 are most commonly responsible for PC-1 and that proline substitution mutations at codons 127 or 128 may produce more severe disease

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with KRT16 elisa kit

 Reference Product  PubMed Publications
 LOR elisa kit  >2 publications with KRT16 and LOR
 FLG elisa kit  >2 publications with KRT16 and FLG
 IL6 elisa kit  >1 publications with KRT16 and IL6

Diseases associated with KRT16 elisa kit

 Disease Name  Pubmed Publications
 Pachyonychia Congenita Antibodies  >30 publications with KRT16 and Pachyonychia Congenita
 Inflammation Antibodies  >4 publications with KRT16 and Inflammation
 Carcinoma, Squamous Cell Antibodies  >3 publications with KRT16 and Carcinoma, Squamous Cell
 Keratoderma, Palmoplantar, Epidermolytic Antibodies  >3 publications with KRT16 and Keratoderma, Palmoplantar, Epidermolytic
 Breast Neoplasms Antibodies  >2 publications with KRT16 and Breast Neoplasms
 Necrosis Antibodies  >2 publications with KRT16 and Necrosis
 Neoplasms, Experimental Antibodies  >2 publications with KRT16 and Neoplasms, Experimental
 PALMOPLANTAR KERATODERMA, NONEPIDERMOLYTIC, FOCAL 1 Antibodies  >2 publications with KRT16 and PALMOPLANTAR KERATODERMA, NONEPIDERMOLYTIC, FOCAL 1
 Precancerous Conditions Antibodies  >2 publications with KRT16 and Precancerous Conditions
 Dermatitis Antibodies  >2 publications with KRT16 and Dermatitis

Organs/Tissues associated with KRT16 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >27 publications with KRT16 and Skin
 Blood Antibodies  >6 publications with KRT16 and Blood
 Mouth Antibodies  >6 publications with KRT16 and Mouth
 Embryonic Tissue Antibodies  >2 publications with KRT16 and Embryonic Tissue
 Kidney Antibodies  >1 publications with KRT16 and Kidney
 Pancreas Antibodies  >1 publications with KRT16 and Pancreas
 Lung Antibodies  >1 publications with KRT16 and Lung
 Brain Antibodies  >1 publications with KRT16 and Brain
 Intestine Antibodies  >1 publications with KRT16 and Intestine
 Larynx Antibodies  >1 publications with KRT16 and Larynx
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