Human CK-18/KRT18 ELISA Kit – Cat# MBS761380

Cytokeratin 18 (CK-18/KRT18) ELISA Kit for Human

Name: Human CK-18/KRT18 ELISA Kit; Cytokeratin 18; MyBioSource
Brand: MyBioSource
SKU: MBS761380
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS761380

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CK- 18/KRT18 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CK- 18/KRT18 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Cytokeratin 18 (CK-18/KRT18), ELISA Kit

Full Product Name

Human Cytokeratin 18 ELISA Kit

Product Gene Name

CK-18/KRT18 elisa kit

Product Synonym Names

KRT18/CK-18/Cytokeratin 18/Keratin; type I cytoskeletal 18/Keratin-18/K18/Cell proliferation-inducing gene 46 protein/Cytokeratin-18

Species Reactivity

Human

Assay Type

Sandwich ELISA, Double Antibody

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

78.125-5000pg/ml

Sensitivity

46.875pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of CK-18/KRT18 . No significant cross-reactivity or interference between CK-18/KRT18 and analogues was observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CK- 18/KRT18 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CK- 18/KRT18 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%

Molecular Weight

48,058 Da

OMIM

148070

3D Structure

ModBase 3D Structure for P05783

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of CK-18/KRT18 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CK-18/KRT18 purchase

MBS761380 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cytokeratin 18 (CK-18/KRT18) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CK-18/KRT18. The ELISA analytical biochemical technique of the MBS761380 kit is based on CK-18/KRT18 antibody-CK-18/KRT18 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CK-18/KRT18 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CK-18/KRT18. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CK-18/KRT18. It may not necessarily be applicable to this product.

NCBI GI #

34037

NCBI GeneID

3875

NCBI Accession #

CAA31375.1

UniProt Primary Accession #

P05783

UniProt Secondary Accession #

Q53G38; Q5U0N8; Q9BW26

UniProt Related Accession #

P05783

NCBI Official Full Name

cytokeratin 18

NCBI Official Synonym Full Names

keratin 18, type I

NCBI Official Symbol

KRT18

NCBI Official Synonym Symbols

K18; CK-18; CYK18

UniProt Protein Name

Keratin, type I cytoskeletal 18

UniProt Synonym Protein Names

Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18

Protein Family

Keratin

UniProt Gene Name

KRT18

UniProt Synonym Gene Names

CYK18; CK-18; K18

UniProt Entry Name

K1C18_HUMAN

NCBI Protein Information

keratin, type I cytoskeletal 18

NCBI Summary for CK-18/KRT18

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

UniProt Comments for CK-18/KRT18

K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis.

Protein type: Nucleolus; Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cytoplasm; intermediate filament; keratin filament; microtubule organizing center; nucleolus; perinuclear region of cytoplasm

Molecular Function: protein binding; structural molecule activity

Biological Process: anatomical structure morphogenesis; cell cycle; Golgi to plasma membrane CFTR protein transport; intermediate filament cytoskeleton organization and biogenesis; negative regulation of apoptosis; tumor necrosis factor-mediated signaling pathway; viral reproduction

Disease: Cirrhosis, Familial

Research Articles on CK-18/KRT18

1. No evidence of association between polymorphisms in KRT18 and sporadic amyotrophic lateral sclerosis in Han Chinese

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CK-18/KRT18 elisa kit

Reference Product	PubMed Publications
KRT8 elisa kit	>60 publications with CK-18/KRT18 and KRT8
EGR1 elisa kit	>1 publications with CK-18/KRT18 and EGR1
TCHP elisa kit	>1 publications with CK-18/KRT18 and TCHP
CASP6 elisa kit	>1 publications with CK-18/KRT18 and CASP6

Pathways associated with CK-18/KRT18 elisa kit

Products by Pathway	Pathway Diagram
Caspase Cascade In Apoptosis Pathway antibodies	Caspase Cascade In Apoptosis Pathway Diagram
EGFR1 Signaling Pathway antibodies	EGFR1 Signaling Pathway Diagram
Pathogenic Escherichia Coli Infection Pathway antibodies	Pathogenic Escherichia Coli Infection Pathway Diagram
Pathogenic Escherichia Coli Infection Pathway antibodies	Pathogenic Escherichia Coli Infection Pathway Diagram
Pathogenic Escherichia Coli Infection Pathway antibodies	Pathogenic Escherichia Coli Infection Pathway Diagram

Diseases associated with CK-18/KRT18 elisa kit

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>38 publications with CK-18/KRT18 and Liver Diseases
Carcinoma Antibodies	>34 publications with CK-18/KRT18 and Carcinoma
Breast Neoplasms Antibodies	>17 publications with CK-18/KRT18 and Breast Neoplasms
Neoplasm Metastasis Antibodies	>16 publications with CK-18/KRT18 and Neoplasm Metastasis
Adenocarcinoma Antibodies	>15 publications with CK-18/KRT18 and Adenocarcinoma
Fibrosis Antibodies	>15 publications with CK-18/KRT18 and Fibrosis
Fatty Liver Antibodies	>15 publications with CK-18/KRT18 and Fatty Liver
Inflammation Antibodies	>11 publications with CK-18/KRT18 and Inflammation
Disease Models, Animal Antibodies	>10 publications with CK-18/KRT18 and Disease Models, Animal
Necrosis Antibodies	>10 publications with CK-18/KRT18 and Necrosis

Organs/Tissues associated with CK-18/KRT18 elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>52 publications with CK-18/KRT18 and Liver
Blood Antibodies	>30 publications with CK-18/KRT18 and Blood
Prostate Antibodies	>9 publications with CK-18/KRT18 and Prostate
Lymph Antibodies	>8 publications with CK-18/KRT18 and Lymph
Lymph Node Antibodies	>8 publications with CK-18/KRT18 and Lymph Node
Muscle Antibodies	>7 publications with CK-18/KRT18 and Muscle
Bone Antibodies	>7 publications with CK-18/KRT18 and Bone
Kidney Antibodies	>7 publications with CK-18/KRT18 and Kidney
Stomach Antibodies	>7 publications with CK-18/KRT18 and Stomach
Eye Antibodies	>7 publications with CK-18/KRT18 and Eye