Keratin, Type II Cytoskeletal 1 (KRT1) ELISA Kit for Human

Name: Human KRT1 ELISA Kit; Keratin, Type II Cytoskeletal 1; MyBioSource
Brand: MyBioSource
SKU: MBS761568
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS761568

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- KRT1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- KRT1 antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Keratin, type II cytoskeletal 1 (KRT1), ELISA Kit

Full Product Name

Human Keratin, type II cytoskeletal 1 ELISA Kit

Product Gene Name

KRT1 elisa kit

Product Synonym Names

KRT1/Type-II keratin Kb1/Keratin-1 (K1)/Hair alpha protein/67 kDa cytokeratin/Cytokeratin-1 (CK-1)/KRTA

Species Reactivity

Human

Assay Type

Sandwich ELISA, Double Antibody

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

0.313-20ng/ml

Sensitivity

0.188ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of KRT1. No significant cross-reactivity or interference between KRT1 and analogues was observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level KRT1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level KRT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%

Molecular Weight

66,039 Da

OMIM

113800

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of KRT1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for KRT1 purchase

MBS761568 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Keratin, type II cytoskeletal 1 (KRT1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing KRT1. The ELISA analytical biochemical technique of the MBS761568 kit is based on KRT1 antibody-KRT1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect KRT1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, KRT1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for KRT1. It may not necessarily be applicable to this product.

NCBI GI #

119395750

NCBI GeneID

3848

NCBI Accession #

NP_006112.3

UniProt Secondary Accession #

P85925; P86104; Q14720; Q6GSJ0; Q9H298; B2RA01

UniProt Related Accession #

P04264

GenBank Nucleotide #

NM_006121.3

NCBI Official Full Name

keratin, type II cytoskeletal 1

NCBI Official Synonym Full Names

keratin 1, type II

NCBI Official Symbol

KRT1

NCBI Official Synonym Symbols

K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK

UniProt Protein Name

Keratin, type II cytoskeletal 1

UniProt Synonym Protein Names

67 kDa cytokeratin; Cytokeratin-1; CK-1; Hair alpha protein; Keratin-1; K1; Type-II keratin Kb1

Protein Family

Keratin

UniProt Gene Name

KRT1

UniProt Synonym Gene Names

KRTA; CK-1; K1

UniProt Entry Name

K2C1_HUMAN

NCBI Protein Information

keratin, type II cytoskeletal 1

NCBI Summary for KRT1

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

UniProt Comments for KRT1

K1: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K10.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cytoskeleton; extracellular matrix; extracellular space; keratin filament; membrane; nucleus; plasma membrane

Molecular Function: carbohydrate binding; protein binding; receptor activity; structural molecule activity

Biological Process: complement activation, lectin pathway; fibrinolysis; negative regulation of inflammatory response; regulation of angiogenesis; response to oxidative stress; retinal homeostasis

Disease: Epidermolytic Hyperkeratosis; Ichthyosis Hystrix, Curth-macklin Type; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis; Keratosis Palmoplantaris Striata Iii; Palmoplantar Keratoderma, Epidermolytic; Palmoplantar Keratoderma, Nonepidermolytic

Research Articles on KRT1

1. These findings indicate that exogenous FABP4 interacts with plasma membrane proteins, specifically CK1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with KRT1 elisa kit

Reference Product	PubMed Publications
KRT10 elisa kit	>59 publications with KRT1 and KRT10
CTNNB1 elisa kit	>11 publications with KRT1 and CTNNB1
KRT2 elisa kit	>9 publications with KRT1 and KRT2
IVL elisa kit	>1 publications with KRT1 and IVL
LOR elisa kit	>1 publications with KRT1 and LOR
LEF1 elisa kit	>1 publications with KRT1 and LEF1

Pathways associated with KRT1 elisa kit

Products by Pathway	Pathway Diagram
Corticotropin-releasing Hormone Pathway antibodies	Corticotropin-releasing Hormone Pathway Diagram
Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies	Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram

Diseases associated with KRT1 elisa kit

Disease Name	Pubmed Publications
Skin Neoplasms Antibodies	>49 publications with KRT1 and Skin Neoplasms
Hyperkeratosis, Epidermolytic Antibodies	>41 publications with KRT1 and Hyperkeratosis, Epidermolytic
Inflammation Antibodies	>35 publications with KRT1 and Inflammation
Carcinoma, Squamous Cell Antibodies	>27 publications with KRT1 and Carcinoma, Squamous Cell
Hyperplasia Antibodies	>26 publications with KRT1 and Hyperplasia
Keratoderma, Palmoplantar, Epidermolytic Antibodies	>23 publications with KRT1 and Keratoderma, Palmoplantar, Epidermolytic
Immune System Diseases Antibodies	>20 publications with KRT1 and Immune System Diseases
Necrosis Antibodies	>12 publications with KRT1 and Necrosis
Cell Transformation, Neoplastic Antibodies	>12 publications with KRT1 and Cell Transformation, Neoplastic
Neoplasms, Experimental Antibodies	>10 publications with KRT1 and Neoplasms, Experimental

Organs/Tissues associated with KRT1 elisa kit

Organ/Tissue Name	Pubmed Publications
Skin Antibodies	>251 publications with KRT1 and Skin
Blood Antibodies	>55 publications with KRT1 and Blood
Mouth Antibodies	>33 publications with KRT1 and Mouth
Thymus Antibodies	>14 publications with KRT1 and Thymus
Lung Antibodies	>12 publications with KRT1 and Lung
Brain Antibodies	>7 publications with KRT1 and Brain
Mammary Gland Antibodies	>7 publications with KRT1 and Mammary Gland
Bladder Antibodies	>4 publications with KRT1 and Bladder
Uterus Antibodies	>3 publications with KRT1 and Uterus
Heart Antibodies	>2 publications with KRT1 and Heart