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Hepatocyte Growth Factor Receptor (c-MET) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS761606

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human HGFR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HGFR antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Hepatocyte growth factor receptor (c-MET), ELISA Kit
Full Product Name
Human Hepatocyte growth factor receptor ELISA Kit
Product Gene Name
c-MET elisa kit
Product Synonym Names
c-MET/HGFR
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
1.563-100ng/ml
Sensitivity
0.938ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
85,745 Da
OMIM
114550
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of c-MET elisa kit

Human c-MET ELISA Kit – Cat# MBS761606

Typical Standard Curve of c-MET elisa kit

Human c-MET ELISA Kit, Typical Standard Curve – Cat# MBS761606

Other Notes

Small volumes of c-MET elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for c-MET purchase

MBS761606 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hepatocyte growth factor receptor (c-MET) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing c-MET. The ELISA analytical biochemical technique of the MBS761606 kit is based on c-MET antibody-c-MET antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect c-MET antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, c-MET. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for c-MET. It may not necessarily be applicable to this product.

NCBI GI #
188595716
NCBI GeneID
4233
NCBI Accession #
NP_001120972.1
UniProt Secondary Accession #
O60366; Q12875; Q9UDX7; Q9UPL8; A1L467; B5A932; E7EQ94
UniProt Related Accession #
P08581
GenBank Nucleotide #
NM_001127500.1
NCBI Official Full Name
hepatocyte growth factor receptor isoform a
NCBI Official Synonym Full Names
MET proto-oncogene, receptor tyrosine kinase
NCBI Official Symbol
MET
NCBI Official Synonym Symbols
HGFR; AUTS9; RCCP2; c-Met; DFNB97
UniProt Protein Name
Hepatocyte growth factor receptor
UniProt Synonym Protein Names
HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met
Protein Family
Hepatocyte growth factor receptor
UniProt Gene Name
MET
UniProt Synonym Gene Names
HGF receptor; SF receptor
UniProt Entry Name
MET_HUMAN
NCBI Protein Information
hepatocyte growth factor receptor

NCBI Summary for c-MET

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for c-MET

Met: a proto-oncogenic receptor tyrosine kinase with high affinity for hepatocyte growth factor. The primary single chain precursor protein is post-translationally cleaved to produce the 45 kDa alpha- and 145 kDa beta-subunits, which are disulfide linked to form the mature receptor. Ligand-binding induces autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase. Activating point mutations cause hereditary papillary renal carcinoma. Mutations also seen in sporadic renal cell carcinoma and childhood hepatocellular carcinoma. Upregulation in carcinomas and sarcomas correlates with metastasis and poor outcome. Some gastric carcinomas harbor a translocation that creates an activated TPR-Met fusion protein. A small molecule inhibitor (PHA-665752) shows an effect in gastric carcinoma xenografts. Inhibitors: SU11274, PHA-665752, mAbs. Two alternatively spliced human isoforms have been reported.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Met family; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: basal plasma membrane; cell surface; integral to plasma membrane; plasma membrane

Molecular Function: hepatocyte growth factor receptor activity; protein binding; protein phosphatase binding; protein-tyrosine kinase activity

Biological Process: branching morphogenesis of a tube; cell proliferation; endothelial cell morphogenesis; positive chemotaxis; positive regulation of transcription from RNA polymerase II promoter; signal transduction

Disease: Deafness, Autosomal Recessive 97; Hepatocellular Carcinoma; Osteofibrous Dysplasia, Susceptibility To; Renal Cell Carcinoma, Papillary, 1
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Research Articles on c-MET

1. This study provides an assessment of tumor cMET gene copy number changes and protein overexpression incidence in a cohort of Chinese GC patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with c-MET elisa kit

 Reference Product  PubMed Publications
 HGF elisa kit  >2924 publications with c-MET and HGF
 CTNNB1 elisa kit  >245 publications with c-MET and CTNNB1
 UBC elisa kit  >185 publications with c-MET and UBC
 GRB2 elisa kit  >119 publications with c-MET and GRB2
 GAB1 elisa kit  >104 publications with c-MET and GAB1
 CBL elisa kit  >70 publications with c-MET and CBL
 CDH1 elisa kit  >43 publications with c-MET and CDH1
 PTPN11 elisa kit  >22 publications with c-MET and PTPN11
 PLXNB1 elisa kit  >20 publications with c-MET and PLXNB1
 PTPN1 elisa kit  >8 publications with c-MET and PTPN1

Pathways associated with c-MET elisa kit

 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Arf6 Signaling Events Pathway antibodies  Arf6 Signaling Events Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram

Diseases associated with c-MET elisa kit

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >10000 publications with c-MET and Cardiovascular Diseases
 Carcinoma Antibodies  >5399 publications with c-MET and Carcinoma
 Heart Diseases Antibodies  >4967 publications with c-MET and Heart Diseases
 Depressive Disorder Antibodies  >3468 publications with c-MET and Depressive Disorder
 Inflammation Antibodies  >2727 publications with c-MET and Inflammation
 Adenocarcinoma Antibodies  >2540 publications with c-MET and Adenocarcinoma
 Liver Diseases Antibodies  >2347 publications with c-MET and Liver Diseases
 Kidney Diseases Antibodies  >2046 publications with c-MET and Kidney Diseases
 Breast Neoplasms Antibodies  >1986 publications with c-MET and Breast Neoplasms
 Schizophrenia Antibodies  >1923 publications with c-MET and Schizophrenia

Organs/Tissues associated with c-MET elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >9524 publications with c-MET and Brain
 Heart Antibodies  >6546 publications with c-MET and Heart
 Bone Antibodies  >5201 publications with c-MET and Bone
 Lung Antibodies  >4887 publications with c-MET and Lung
 Vascular Antibodies  >4631 publications with c-MET and Vascular
 Liver Antibodies  >4462 publications with c-MET and Liver
 Muscle Antibodies  >4146 publications with c-MET and Muscle
 Kidney Antibodies  >3216 publications with c-MET and Kidney
 Skin Antibodies  >2546 publications with c-MET and Skin
 Eye Antibodies  >2027 publications with c-MET and Eye
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