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Neuropeptide Ff Receptor 2 ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS761667

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to NPFFR2. During the reaction,NPFFR2 in the sample or standard competes with a fixed amount… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Neuropeptide FF receptor 2 (NPFFR2), ELISA Kit
Full Product Name
Human Neuropeptide FF receptor 2 ELISA Kit
Product Gene Name
NPFFR2 elisa kit
Product Synonym Names
NPFFR2/Neuropeptide FF receptor 2/Neuropeptide G-protein coupled receptor/G-protein coupled receptor 74/G-protein coupled receptor HLWAR77
Species Reactivity
Human
Assay Type
Competitive ELISA, Coated with Antigen
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.625-40ng/ml
Sensitivity
0.375ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
15,055 Da
OMIM
607449
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of NPFFR2 elisa kit

Human ELISA Kit – Cat# MBS761667

Typical Standard Curve of NPFFR2 elisa kit

Human ELISA Kit, Typical Standard Curve – Cat# MBS761667

Other Notes

Small volumes of NPFFR2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NPFFR2 purchase

MBS761667 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neuropeptide FF receptor 2 (NPFFR2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NPFFR2. The ELISA analytical biochemical technique of the MBS761667 kit is based on NPFFR2 antibody-NPFFR2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NPFFR2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NPFFR2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NPFFR2. It may not necessarily be applicable to this product.

NCBI GI #
11907915
NCBI GeneID
10886
NCBI Accession #
AAG41398.1
UniProt Secondary Accession #
Q96RV1; Q9NR49
UniProt Related Accession #
Q9Y5X5
NCBI Official Full Name
neuropeptide FF receptor 2
NCBI Official Synonym Full Names
neuropeptide FF receptor 2
NCBI Official Symbol
NPFFR2
NCBI Official Synonym Symbols
GPR74; NPFF2; NPGPR; HLWAR77
UniProt Protein Name
Neuropeptide FF receptor 2
UniProt Synonym Protein Names
G-protein coupled receptor 74; G-protein coupled receptor HLWAR77; Neuropeptide G-protein coupled receptor
Protein Family
Neuropeptide FF receptor
UniProt Gene Name
NPFFR2
UniProt Synonym Gene Names
GPR74; NPFF2; NPGPR
UniProt Entry Name
NPFF2_HUMAN
NCBI Protein Information
neuropeptide FF receptor 2

NCBI Summary for NPFFR2

This gene encodes a member of a subfamily of G-protein-coupled neuropeptide receptors. This protein is activated by the neuropeptides A-18-amide (NPAF) and F-8-amide (NPFF) and may function in pain modulation and regulation of the opioid system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]
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UniProt Comments for NPFFR2

NPFFR2: Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol- calcium second messenger system. Belongs to the G-protein coupled receptor 1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: actin cytoskeleton; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; neuropeptide receptor activity; opioid receptor binding; peptide binding

Biological Process: cellular response to hormone stimulus; detection of abiotic stimulus; G-protein coupled receptor protein signaling pathway; neuropeptide signaling pathway; regulation of adenylate cyclase activity; regulation of MAPKKK cascade
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Research Articles on NPFFR2

1. The NPFF2 neuropeptide FF receptor phosphorylation sites have been mapped and their role identified in receptor regulation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NPFFR2 elisa kit

 Reference Product  PubMed Publications
 NPFF elisa kit  >6 publications with NPFFR2 and NPFF
 QRFPR elisa kit  >1 publications with NPFFR2 and QRFPR

Diseases associated with NPFFR2 elisa kit

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1 publications with NPFFR2 and Inflammation

Organs/Tissues associated with NPFFR2 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >2 publications with NPFFR2 and Bone
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