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Lysophosphatidylcholine Acyltransferase 2 (LPCAT2) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS761883

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human LPCAT2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human LPCAT2 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Lysophosphatidylcholine acyltransferase 2 (LPCAT2), ELISA Kit
Full Product Name
Human Lysophosphatidylcholine acyltransferase 2 ELISA Kit
Product Gene Name
LPCAT2 elisa kit
Product Synonym Names
LPCAT2/LPC acyltransferase 2/LPCAT-2/LysoPC acyltransferase 2/Lysophosphatidic acid acyltransferase alpha/LPAAT-alpha/Acetyl-CoA,lyso-PAF acetyltransferase
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
30,895 Da
OMIM
612040
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of LPCAT2 elisa kit

Human LPCAT2 ELISA Kit – Cat# MBS761883

Typical Standard Curve of LPCAT2 elisa kit

Human LPCAT2 ELISA Kit, Typical Standard Curve – Cat# MBS761883

Other Notes

Small volumes of LPCAT2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LPCAT2 purchase

MBS761883 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Lysophosphatidylcholine acyltransferase 2 (LPCAT2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing LPCAT2. The ELISA analytical biochemical technique of the MBS761883 kit is based on LPCAT2 antibody-LPCAT2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LPCAT2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, LPCAT2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for LPCAT2. It may not necessarily be applicable to this product.

NCBI GI #
47086907
NCBI GeneID
54947
NCBI Accession #
NP_060309.2
UniProt Secondary Accession #
Q6MZJ6; Q9NX23; A3KBM1
UniProt Related Accession #
Q7L5N7
GenBank Nucleotide #
NM_017839.4
NCBI Official Full Name
lysophosphatidylcholine acyltransferase 2
NCBI Official Synonym Full Names
lysophosphatidylcholine acyltransferase 2
NCBI Official Symbol
LPCAT2
NCBI Official Synonym Symbols
AYTL1; AGPAT11; LysoPAFAT
UniProt Protein Name
Lysophosphatidylcholine acyltransferase 2
UniProt Synonym Protein Names
1-acylglycerol-3-phosphate O-acyltransferase 11 (EC:2.3.1.51); 1-AGP acyltransferase 11; 1-AGPAT 11; 1-acylglycerophosphocholine O-acyltransferase; 1-alkylglycerophosphocholine O-acetyltransferase (EC:2.3.1.67); Acetyl-CoA:lyso-platelet-activating factor acetyltransferase; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT; Acyltransferase-like 1; Lysophosphatidic acid acyltransferase alpha; LPAAT-alpha
Protein Family
Lysophosphatidylcholine acyltransferase
UniProt Gene Name
LPCAT2
UniProt Synonym Gene Names
AGPAT11; AYTL1; LPC acyltransferase 2; LPCAT-2; LysoPC acyltransferase 2; 1-AGP acyltransferase 11; 1-AGPAT 11; Acetyl-CoA:lyso-PAF acetyltransferase; Lyso-PAF acetyltransferase; LysoPAFAT; LPAAT-alpha
UniProt Entry Name
PCAT2_HUMAN
NCBI Protein Information
lysophosphatidylcholine acyltransferase 2

NCBI Summary for LPCAT2

This gene encodes a member of the lysophospholipid acyltransferase family. The encoded enzyme may function in two ways: to catalyze the biosynthesis of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) from 1-O-alkyl-sn-glycero-3-phosphocholine, and to catalyze the synthesis of glycerophospholipid precursors from arachidonyl-CoA and lysophosphatidylcholine. The encoded protein may function in membrane biogenesis and production of platelet-activating factor in inflammatory cells. The enzyme may localize to the endoplasmic reticulum and the Golgi. [provided by RefSeq, Feb 2009]
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UniProt Comments for LPCAT2

AYTL1: Possesses both acyltransferase and acetyltransferase activities. Activity is calcium-dependent. Involved in platelet- activating factor (PAF) biosynthesis by catalyzing the conversion of the PAF precursor, 1-O-alkyl-sn-glycero-3-phosphocholine (lyso- PAF) into 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (PAF). Also converts lyso-PAF to 1-alkyl-phosphatidylcholine (PC), a major component of cell membranes and a PAF precursor. Under resting conditions, acyltransferase activity is preferred. Upon acute inflammatory stimulus, acetyltransferase activity is enhanced and PAF synthesis increases. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.67; Membrane protein, integral; EC 2.3.1.23; EC 2.3.1.51; Acetyltransferase

Chromosomal Location of Human Ortholog: 16q12.2

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; Golgi membrane; Golgi stack; integral to membrane; lipid particle

Molecular Function: 1-acylglycerol-3-phosphate O-acyltransferase activity; 1-acylglycerophosphocholine O-acyltransferase activity; 1-alkylglycerophosphocholine O-acetyltransferase activity; calcium ion binding

Biological Process: glycerophospholipid biosynthetic process; phospholipid metabolic process; platelet activating factor biosynthetic process
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Research Articles on LPCAT2

1. three of these five genes (CXCL14, ITGAX, and LPCAT2) harbored polymorphisms associated with aggressive disease development in a human GWAS cohort consisting of 1,172 prostate cancer patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with LPCAT2 elisa kit

 Reference Product  PubMed Publications
 LPCAT4 elisa kit  >1 publications with LPCAT2 and LPCAT4
 LYPLA1 elisa kit  >1 publications with LPCAT2 and LYPLA1

Pathways associated with LPCAT2 elisa kit

 Products by Pathway  Pathway Diagram
 Acyl Chain Remodelling Of PC Pathway antibodies  Acyl Chain Remodelling Of PC Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Ether Lipid Metabolism Pathway antibodies  Ether Lipid Metabolism Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Phospholipid Metabolism Pathway antibodies  Phospholipid Metabolism Pathway Diagram

Diseases associated with LPCAT2 elisa kit

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >5 publications with LPCAT2 and Inflammation
 Disease Models, Animal Antibodies  >3 publications with LPCAT2 and Disease Models, Animal
 Liver Diseases Antibodies  >3 publications with LPCAT2 and Liver Diseases
 Fatty Liver Antibodies  >2 publications with LPCAT2 and Fatty Liver
 Liver Cirrhosis Antibodies  >2 publications with LPCAT2 and Liver Cirrhosis
 Kidney Diseases Antibodies  >1 publications with LPCAT2 and Kidney Diseases
 Non-alcoholic Fatty Liver Disease Antibodies  >1 publications with LPCAT2 and Non-alcoholic Fatty Liver Disease
 Uterine Cervical Neoplasms Antibodies  >1 publications with LPCAT2 and Uterine Cervical Neoplasms
 Lung Diseases Antibodies  >1 publications with LPCAT2 and Lung Diseases
 Fibrosis Antibodies  >1 publications with LPCAT2 and Fibrosis

Organs/Tissues associated with LPCAT2 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >3 publications with LPCAT2 and Blood
 Bone Marrow Antibodies  >2 publications with LPCAT2 and Bone Marrow
 Prostate Antibodies  >2 publications with LPCAT2 and Prostate
 Nerve Antibodies  >1 publications with LPCAT2 and Nerve
 Ovary Antibodies  >1 publications with LPCAT2 and Ovary
 Intestine Antibodies  >1 publications with LPCAT2 and Intestine
 Thyroid Antibodies  >1 publications with LPCAT2 and Thyroid
 Lung Antibodies  >1 publications with LPCAT2 and Lung
 Embryonic Tissue Antibodies  >1 publications with LPCAT2 and Embryonic Tissue
 Ganglia Antibodies  >1 publications with LPCAT2 and Ganglia
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