Growth/Differentiation Factor 8 (MSTN) ELISA Kit for Human

Name: Human MSTN ELISA Kit; Growth/Differentiation Factor 8; MyBioSource
Brand: MyBioSource
SKU: MBS761907
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS761907

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MSTN antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MSTN antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Growth/differentiation factor 8 (MSTN), ELISA Kit

Full Product Name

Human Growth/differentiation factor 8 ELISA Kit

Product Gene Name

MSTN elisa kit

Product Synonym Names

MSTN (Myostatin)/GDF-8/GDF8/growth differentiation factor 8/growth,differentiation factor 8

Species Reactivity

Human

Assay Type

Sandwich ELISA, Double Antibody

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

31.25-2000pg/ml

Sensitivity

18.75pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of MSTN . No significant cross-reactivity or interference between MSTN and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between MSTN and all the analogues, therefore, cross reaction may still exist.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MSTN were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MSTN were tested 20 times on one plate, respectively. Intra-Assay: CV<8%

Molecular Weight

42,750 Da

OMIM

601788

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of MSTN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MSTN purchase

MBS761907 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Growth/differentiation factor 8 (MSTN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MSTN. The ELISA analytical biochemical technique of the MBS761907 kit is based on MSTN antibody-MSTN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MSTN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MSTN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MSTN. It may not necessarily be applicable to this product.

NCBI GI #

4885259

NCBI GeneID

2660

NCBI Accession #

NP_005250.1

UniProt Secondary Accession #

Q6B0H2; A1C2J7; A1C2K0

UniProt Related Accession #

O14793

GenBank Nucleotide #

NM_005259.2

NCBI Official Full Name

growth/differentiation factor 8 preproprotein

NCBI Official Synonym Full Names

myostatin

NCBI Official Symbol

MSTN

NCBI Official Synonym Symbols

GDF8; MSLHP

UniProt Protein Name

Growth/differentiation factor 8

UniProt Synonym Protein Names

Myostatin

Protein Family

Growth/differentiation factor

UniProt Gene Name

MSTN

UniProt Synonym Gene Names

GDF8; GDF-8

UniProt Entry Name

GDF8_HUMAN

NCBI Protein Information

growth/differentiation factor 8

NCBI Summary for MSTN

This gene encodes a member of the TGF-beta (transforming growth factor-beta) family of proteins. The encoded preproprotein is proteolytically processed to generate an N-terminal propeptide and a C-terminal subunit. The C-terminal subunits form the mature homodimer, which negatively regulates skeletal muscle cell proliferation and differentiation. Mutations in this gene are associated with increased skeletal muscle mass in humans and other mammals. [provided by RefSeq, Nov 2015]

UniProt Comments for MSTN

MSTN: Acts specifically as a negative regulator of skeletal muscle growth. Defects in MSTN are the cause of muscle hypertrophy (MSLHP). MSLHP is a condition characterized by increased muscle bulk and strength. Affected individuals are exceptionally strong. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q32.2

Cellular Component: cytoplasm; extracellular space

Molecular Function: cytokine activity; growth factor activity; heparin binding; identical protein binding; protein binding; receptor binding; transforming growth factor beta receptor binding

Biological Process: cell development; muscle development; muscle maintenance; negative regulation of insulin receptor signaling pathway; negative regulation of kinase activity; negative regulation of muscle hypertrophy; negative regulation of myoblast differentiation; negative regulation of protein kinase B signaling cascade; negative regulation of skeletal muscle growth; ovulation cycle process; positive regulation of defense response to virus by host; positive regulation of transcription, DNA-dependent; regulation of apoptosis; regulation of MAPKKK cascade; response to electrical stimulus; response to estrogen stimulus; response to ethanol; response to gravity; response to heat; response to muscle activity; response to testosterone stimulus; skeletal muscle atrophy; skeletal muscle regeneration; transforming growth factor beta receptor signaling pathway

Disease: Muscle Hypertrophy

Research Articles on MSTN

1. Lower serum myostatin independently associated with MetS, central obesity, low HDL-C, and high triglycerides after adjustment. Higher serum myostatin is associated with favorable metabolic profiles.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MSTN elisa kit

Reference Product	PubMed Publications
MYOD1 elisa kit	>26 publications with MSTN and MYOD1
FSTL3 elisa kit	>23 publications with MSTN and FSTL3
ACVR2B elisa kit	>12 publications with MSTN and ACVR2B
DCN elisa kit	>8 publications with MSTN and DCN
WFIKKN2 elisa kit	>3 publications with MSTN and WFIKKN2
ACVR1B elisa kit	>3 publications with MSTN and ACVR1B
TGFBR1 elisa kit	>2 publications with MSTN and TGFBR1
AKT1 elisa kit	>2 publications with MSTN and AKT1
WNT4 elisa kit	>2 publications with MSTN and WNT4
MYL1 elisa kit	>1 publications with MSTN and MYL1

Pathways associated with MSTN elisa kit

Products by Pathway	Pathway Diagram
Hypertrophy Model Pathway antibodies	Hypertrophy Model Pathway Diagram

Diseases associated with MSTN elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>165 publications with MSTN and Nervous System Diseases
Hypertrophy Antibodies	>161 publications with MSTN and Hypertrophy
Atrophy Antibodies	>131 publications with MSTN and Atrophy
Weight Loss Antibodies	>52 publications with MSTN and Weight Loss
Hyperplasia Antibodies	>38 publications with MSTN and Hyperplasia
Insulin Resistance Antibodies	>37 publications with MSTN and Insulin Resistance
Disease Models, Animal Antibodies	>37 publications with MSTN and Disease Models, Animal
Necrosis Antibodies	>22 publications with MSTN and Necrosis
Inflammation Antibodies	>20 publications with MSTN and Inflammation
Kidney Diseases Antibodies	>19 publications with MSTN and Kidney Diseases

Organs/Tissues associated with MSTN elisa kit

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>792 publications with MSTN and Muscle
Connective Tissue Antibodies	>57 publications with MSTN and Connective Tissue
Heart Antibodies	>56 publications with MSTN and Heart
Embryonic Tissue Antibodies	>47 publications with MSTN and Embryonic Tissue
Brain Antibodies	>39 publications with MSTN and Brain
Eye Antibodies	>9 publications with MSTN and Eye
Lung Antibodies	>8 publications with MSTN and Lung
Placenta Antibodies	>7 publications with MSTN and Placenta