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Carnitine O-Palmitoyltransferase 1, Liver Isoform (CPT1A) ELISA Kit for Rat

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS762015

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Rat Cpt1a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Cpt1a antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Carnitine O-palmitoyltransferase 1, liver isoform (Cpt1a), ELISA Kit
Full Product Name
Rat Carnitine O-palmitoyltransferase 1, liver isoform ELISA Kit
Product Gene Name
Cpt1a elisa kit
Product Synonym Names
Cpt1a/CPT1-L/Carnitine O-palmitoyltransferase 1; liver isoform/CPT1A/CPT I/CPTI-L/Carnitine palmitoyltransferase 1A
Species Reactivity
Rat
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
15.625-1000pg/ml
Sensitivity
9.375pg/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
86,239 Da
OMIM
255120
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of Cpt1a elisa kit

Rat CPT1A ELISA Kit – Cat# MBS762015

Typical Standard Curve of Cpt1a elisa kit

Rat CPT1A ELISA Kit, Typical Standard Curve – Cat# MBS762015

Other Notes

Small volumes of Cpt1a elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Cpt1a purchase

MBS762015 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Carnitine O-palmitoyltransferase 1, liver isoform (Cpt1a) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Cpt1a. The ELISA analytical biochemical technique of the MBS762015 kit is based on Cpt1a antibody-Cpt1a antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cpt1a antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Cpt1a. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Cpt1a. It may not necessarily be applicable to this product.

NCBI GI #
73623030
NCBI GeneID
1374
NCBI Accession #
NP_001867.2
UniProt Secondary Accession #
Q8TCU0; Q9BWK0
UniProt Related Accession #
P50416
GenBank Nucleotide #
NM_001876.3
NCBI Official Full Name
carnitine O-palmitoyltransferase 1, liver isoform isoform 1
NCBI Official Synonym Full Names
carnitine palmitoyltransferase 1A
NCBI Official Symbol
CPT1A
NCBI Official Synonym Symbols
CPT1; CPT1-L; L-CPT1
UniProt Protein Name
Carnitine O-palmitoyltransferase 1, liver isoform
UniProt Synonym Protein Names
Carnitine O-palmitoyltransferase I, liver isoform; CPT I; CPTI-L; Carnitine palmitoyltransferase 1A
Protein Family
Carnitine O-palmitoyltransferase
UniProt Gene Name
CPT1A
UniProt Synonym Gene Names
CPT1; CPT1-L; CPT I; CPTI-L
UniProt Entry Name
CPT1A_HUMAN
NCBI Protein Information
carnitine O-palmitoyltransferase 1, liver isoform

NCBI Summary for Cpt1a

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for Cpt1a

CPT1A: Belongs to the carnitine/choline acetyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Transferase; Mitochondrial; Membrane protein, integral; EC 2.3.1.21; Lipid Metabolism - fatty acid

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: integral to mitochondrial outer membrane; membrane; mitochondrial inner membrane; mitochondrial outer membrane; mitochondrion

Molecular Function: carnitine O-palmitoyltransferase activity; identical protein binding

Biological Process: carnitine metabolic process; carnitine shuttle; cellular lipid metabolic process; circadian rhythm; eating behavior; epithelial cell differentiation; fatty acid beta-oxidation; glucose metabolic process; long-chain fatty acid metabolic process; positive regulation of fatty acid beta-oxidation; protein homooligomerization; regulation of insulin secretion; response to drug; response to organic cyclic substance; triacylglycerol metabolic process

Disease: Carnitine Palmitoyltransferase I Deficiency
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Research Articles on Cpt1a

1. Targeting the leukemia cell metabolism by the CPT1a inhibition

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Cpt1a elisa kit

 Reference Product  PubMed Publications
 ACOX1 elisa kit  >20 publications with Cpt1a and ACOX1
 PPARA elisa kit  >11 publications with Cpt1a and PPARA
 LPL elisa kit  >10 publications with Cpt1a and LPL
 PPARGC1A elisa kit  >9 publications with Cpt1a and PPARGC1A
 ACSL1 elisa kit  >8 publications with Cpt1a and ACSL1
 FABP1 elisa kit  >6 publications with Cpt1a and FABP1
 EHHADH elisa kit  >2 publications with Cpt1a and EHHADH
 ACSL5 elisa kit  >1 publications with Cpt1a and ACSL5

Pathways associated with Cpt1a elisa kit

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Circadian Clock Pathway antibodies  Circadian Clock Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 Fatty Acid Beta Oxidation Pathway antibodies  Fatty Acid Beta Oxidation Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram

Diseases associated with Cpt1a elisa kit

 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >113 publications with Cpt1a and Fatty Liver
 Liver Diseases Antibodies  >48 publications with Cpt1a and Liver Diseases
 Insulin Resistance Antibodies  >41 publications with Cpt1a and Insulin Resistance
 Inflammation Antibodies  >20 publications with Cpt1a and Inflammation
 Weight Gain Antibodies  >12 publications with Cpt1a and Weight Gain
 Atherosclerosis Antibodies  >11 publications with Cpt1a and Atherosclerosis
 Hepatitis Antibodies  >10 publications with Cpt1a and Hepatitis
 Dyslipidemias Antibodies  >10 publications with Cpt1a and Dyslipidemias
 Cardiovascular Diseases Antibodies  >9 publications with Cpt1a and Cardiovascular Diseases
 Diabetes Mellitus Antibodies  >9 publications with Cpt1a and Diabetes Mellitus

Organs/Tissues associated with Cpt1a elisa kit

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >145 publications with Cpt1a and Liver
 Blood Antibodies  >76 publications with Cpt1a and Blood
 Muscle Antibodies  >46 publications with Cpt1a and Muscle
 Brain Antibodies  >23 publications with Cpt1a and Brain
 Connective Tissue Antibodies  >16 publications with Cpt1a and Connective Tissue
 Kidney Antibodies  >10 publications with Cpt1a and Kidney
 Vascular Antibodies  >9 publications with Cpt1a and Vascular
 Thyroid Antibodies  >5 publications with Cpt1a and Thyroid
 Pancreas Antibodies  >5 publications with Cpt1a and Pancreas
 Lung Antibodies  >3 publications with Cpt1a and Lung
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