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Oxysterols Receptor LXR-Beta (NR1H2) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS762074

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human NR1H2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human NR1H2 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Oxysterols receptor LXR-beta (NR1H2), ELISA Kit
Full Product Name
Human Oxysterols receptor LXR-beta ELISA Kit
Product Gene Name
NR1H2 elisa kit
Product Synonym Names
NR1H2 (Oxysterols receptor LXR-beta)/LXRB/NER/UNR/Ubiquitously-expressed nuclear receptor/Nuclear receptor subfamily 1 group H member 2/Liver X receptor beta/Nuclear receptor NER
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
39,920 Da
OMIM
600380
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of NR1H2 elisa kit

Human NR1H2 ELISA Kit – Cat# MBS762074

Typical Standard Curve of NR1H2 elisa kit

Human NR1H2 ELISA Kit, Typical Standard Curve – Cat# MBS762074

Other Notes

Small volumes of NR1H2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NR1H2 purchase

MBS762074 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Oxysterols receptor LXR-beta (NR1H2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NR1H2. The ELISA analytical biochemical technique of the MBS762074 kit is based on NR1H2 antibody-NR1H2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NR1H2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NR1H2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NR1H2. It may not necessarily be applicable to this product.

NCBI GI #
375331888
NCBI GeneID
7376
NCBI Accession #
NP_001243576.1
UniProt Secondary Accession #
Q12970; Q5I0Y1; A8K490; B4DNM6; E7EWA6
UniProt Related Accession #
P55055
GenBank Nucleotide #
NM_001256647.1
NCBI Official Full Name
oxysterols receptor LXR-beta isoform 2
NCBI Official Synonym Full Names
nuclear receptor subfamily 1 group H member 2
NCBI Official Symbol
NR1H2
NCBI Official Synonym Symbols
NER; UNR; LXRB; LXR-b; NER-I; RIP15
UniProt Protein Name
Oxysterols receptor LXR-beta
UniProt Synonym Protein Names
Liver X receptor beta; Nuclear receptor NER; Nuclear receptor subfamily 1 group H member 2; Ubiquitously-expressed nuclear receptor
Protein Family
Oxysterols receptor
UniProt Gene Name
NR1H2
UniProt Synonym Gene Names
LXRB; NER; UNR
UniProt Entry Name
NR1H2_HUMAN
NCBI Protein Information
oxysterols receptor LXR-beta

NCBI Summary for NR1H2

The liver X receptors, LXRA (NR1H3; MIM 602423) and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements (summary by Korf et al., 2009 [PubMed 19436111]).[supplied by OMIM, Jan 2010]
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UniProt Comments for NR1H2

LXR-beta: Orphan receptor. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Forms a heterodimer with RXR. Ubiquitous. Belongs to the nuclear hormone receptor family. NR1 subfamily.

Protein type: Nuclear receptor

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: apolipoprotein A-I receptor binding; ATPase binding; DNA binding; ligand-dependent nuclear receptor activity; protein binding; retinoid X receptor binding; steroid hormone receptor activity; zinc ion binding

Biological Process: cellular lipid metabolic process; cholesterol homeostasis; gene expression; negative regulation of lipid transport; negative regulation of pinocytosis; negative regulation of proteolysis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of cellular protein metabolic process; positive regulation of cholesterol transport; positive regulation of fatty acid biosynthetic process; positive regulation of lipoprotein lipase activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; retinoic acid receptor signaling pathway; steroid hormone mediated signaling; transcription initiation from RNA polymerase II promoter
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Research Articles on NR1H2

1. Destabilization of the torsioned conformation of a ligand side chain inverts the LXRbeta activity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NR1H2 elisa kit

 Reference Product  PubMed Publications
 ABCA1 elisa kit  >10 publications with NR1H2 and ABCA1
 NR0B2 elisa kit  >3 publications with NR1H2 and NR0B2
 NR2F1 elisa kit  >1 publications with NR1H2 and NR2F1
 PPARA elisa kit  >1 publications with NR1H2 and PPARA

Pathways associated with NR1H2 elisa kit

 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Insulin Resistance Pathway antibodies  Insulin Resistance Pathway Diagram
 Nuclear Receptor Transcription Pathway antibodies  Nuclear Receptor Transcription Pathway Diagram
 Nuclear Receptors Pathway antibodies  Nuclear Receptors Pathway Diagram
 SREBP Signalling Pathway antibodies  SREBP Signalling Pathway Diagram

Diseases associated with NR1H2 elisa kit

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >9 publications with NR1H2 and Liver Diseases
 Fatty Liver Antibodies  >9 publications with NR1H2 and Fatty Liver
 Inflammation Antibodies  >7 publications with NR1H2 and Inflammation
 Atherosclerosis Antibodies  >6 publications with NR1H2 and Atherosclerosis
 Disease Models, Animal Antibodies  >4 publications with NR1H2 and Disease Models, Animal
 Hypercholesterolemia Antibodies  >4 publications with NR1H2 and Hypercholesterolemia
 Dyslipidemias Antibodies  >3 publications with NR1H2 and Dyslipidemias
 Vascular Diseases Antibodies  >3 publications with NR1H2 and Vascular Diseases
 Insulin Resistance Antibodies  >3 publications with NR1H2 and Insulin Resistance
 Hypertriglyceridemia Antibodies  >2 publications with NR1H2 and Hypertriglyceridemia

Organs/Tissues associated with NR1H2 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >51 publications with NR1H2 and Liver
 Blood Antibodies  >8 publications with NR1H2 and Blood
 Brain Antibodies  >7 publications with NR1H2 and Brain
 Thyroid Antibodies  >5 publications with NR1H2 and Thyroid
 Kidney Antibodies  >3 publications with NR1H2 and Kidney
 Heart Antibodies  >3 publications with NR1H2 and Heart
 Ovary Antibodies  >3 publications with NR1H2 and Ovary
 Pancreas Antibodies  >3 publications with NR1H2 and Pancreas
 Skin Antibodies  >3 publications with NR1H2 and Skin
 Intestine Antibodies  >2 publications with NR1H2 and Intestine
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