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GATA Binding Protein 3 (GATA3) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS762112

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse GATA3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse GATA3 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GATA Binding Protein 3 (GATA3), ELISA Kit
Full Product Name
Mouse GATA Binding Protein 3 ELISA Kit
Product Gene Name
GATA3 elisa kit
Product Synonym Names
GATA binding protein 3/GATA-binding factor 3/GATA-binding protein 3/HDRMGC5199/MGC2346/MGC5445/trans-acting T-cell-specific transcription factor GATA-3
Species Reactivity
Mouse
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
15.625-1000pg/ml
Sensitivity
9.375pg/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
48,045 Da
OMIM
131320
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of GATA3 elisa kit

Mouse GATA3 ELISA Kit – Cat# MBS762112

Typical Standard Curve of GATA3 elisa kit

Mouse GATA3 ELISA Kit, Typical Standard Curve – Cat# MBS762112

Other Notes

Small volumes of GATA3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GATA3 purchase

MBS762112 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the GATA Binding Protein 3 (GATA3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GATA3. The ELISA analytical biochemical technique of the MBS762112 kit is based on GATA3 antibody-GATA3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GATA3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GATA3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GATA3. It may not necessarily be applicable to this product.

NCBI GI #
13905020
NCBI GeneID
2625
NCBI Accession #
AAH06793.1
UniProt Secondary Accession #
Q5VWG7; Q5VWG8; Q96J16
UniProt Related Accession #
P23771
NCBI Official Full Name
GATA binding protein 3
NCBI Official Synonym Full Names
GATA binding protein 3
NCBI Official Symbol
GATA3
NCBI Official Synonym Symbols
HDR; HDRS
UniProt Protein Name
Trans-acting T-cell-specific transcription factor GATA-3
UniProt Synonym Protein Names
GATA-binding factor 3
Protein Family
GATA-binding factor
UniProt Gene Name
GATA3
UniProt Entry Name
GATA3_HUMAN
NCBI Protein Information
trans-acting T-cell-specific transcription factor GATA-3

NCBI Summary for GATA3

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia. [provided by RefSeq, Nov 2009]
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UniProt Comments for GATA3

GATA3: a transcriptional regulator central in Th2 cell differentiation. Inhibits breast cancer growth and pulmonary breast cancer metastasis. Represses INK4C transcription, constrains luminal progenitor cell expansion, and suppresses luminal tumorigenesis in the mammary gland. High GATA3 and low INK4C expression predicts a favorable patient outcome in luminal A type breast tumors. IFN-lambda1 (IL-29) inhibits GATA3 expression and suppresses Th2 responses in human T cells. GATA3 haploinsufficiency leads to HDR (hypoparathyroidism, deafness, and renal dysplasia) syndrome. Two alternatively spliced human isoforms have been described.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 10p15

Cellular Component: nuclear chromatin; nucleolus; nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin binding; DNA binding; protein binding; transcription coactivator activity; transcription factor activity; transcription factor binding

Biological Process: anatomical structure formation; anatomical structure morphogenesis; blood coagulation; cell fate commitment; cell fate determination; defense response; ear development; gut development; heart development; kidney development; male gonad development; mesonephros development; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of fat cell differentiation; negative regulation of inflammatory response; negative regulation of mammary gland epithelial cell proliferation; negative regulation of transcription, DNA-dependent; norepinephrine biosynthetic process; organ morphogenesis; pharyngeal system development; phosphoinositide 3-kinase cascade; positive regulation of interleukin-4 production; positive regulation of protein kinase B signaling cascade; positive regulation of signal transduction; positive regulation of T cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of cytokine biosynthetic process; response to estrogen stimulus; response to virus; signal transduction; sympathetic nervous system development; T cell receptor signaling pathway; TOR signaling pathway; transcription from RNA polymerase II promoter; uterus development

Disease: Hypoparathyroidism, Sensorineural Deafness, And Renal Disease
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Research Articles on GATA3

1. discuss mutation of GATA3 in breast cancer and the potential mechanism(s) by which mutation may lead to a growth advantage in cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GATA3 elisa kit

 Reference Product  PubMed Publications
 TBX21 elisa kit  >325 publications with GATA3 and TBX21
 FOXP3 elisa kit  >214 publications with GATA3 and FOXP3
 IL13 elisa kit  >105 publications with GATA3 and IL13
 IL10 elisa kit  >84 publications with GATA3 and IL10
 IL4 elisa kit  >78 publications with GATA3 and IL4
 NOTCH1 elisa kit  >33 publications with GATA3 and NOTCH1
 IL5 elisa kit  >23 publications with GATA3 and IL5
 MYB elisa kit  >16 publications with GATA3 and MYB
 LMO1 elisa kit  >8 publications with GATA3 and LMO1

Pathways associated with GATA3 elisa kit

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Factors Involved In Megakaryocyte Development And Platelet Production Pathway antibodies  Factors Involved In Megakaryocyte Development And Platelet Production Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 IL27-mediated Signaling Events Pathway antibodies  IL27-mediated Signaling Events Pathway Diagram
 Inflammatory Bowel Disease (IBD) Pathway antibodies  Inflammatory Bowel Disease (IBD) Pathway Diagram
 Inflammatory Bowel Disease (IBD) Pathway antibodies  Inflammatory Bowel Disease (IBD) Pathway Diagram
 Notch Signaling Pathway antibodies  Notch Signaling Pathway Diagram

Diseases associated with GATA3 elisa kit

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >285 publications with GATA3 and Inflammation
 Lung Diseases Antibodies  >223 publications with GATA3 and Lung Diseases
 Carcinoma Antibodies  >168 publications with GATA3 and Carcinoma
 Breast Neoplasms Antibodies  >163 publications with GATA3 and Breast Neoplasms
 Disease Models, Animal Antibodies  >149 publications with GATA3 and Disease Models, Animal
 Kidney Diseases Antibodies  >74 publications with GATA3 and Kidney Diseases
 Necrosis Antibodies  >62 publications with GATA3 and Necrosis
 Cardiovascular Diseases Antibodies  >51 publications with GATA3 and Cardiovascular Diseases
 Lung Neoplasms Antibodies  >33 publications with GATA3 and Lung Neoplasms
 Neoplasm Metastasis Antibodies  >29 publications with GATA3 and Neoplasm Metastasis

Organs/Tissues associated with GATA3 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1179 publications with GATA3 and Blood
 Lung Antibodies  >192 publications with GATA3 and Lung
 Kidney Antibodies  >132 publications with GATA3 and Kidney
 Skin Antibodies  >94 publications with GATA3 and Skin
 Embryonic Tissue Antibodies  >85 publications with GATA3 and Embryonic Tissue
 Brain Antibodies  >81 publications with GATA3 and Brain
 Thymus Antibodies  >80 publications with GATA3 and Thymus
 Lymph Node Antibodies  >79 publications with GATA3 and Lymph Node
 Liver Antibodies  >70 publications with GATA3 and Liver
 Ear Antibodies  >62 publications with GATA3 and Ear
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