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Protein Wnt-5b (WNT5B) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS762196

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human WNT5B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human WNT5B antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Protein Wnt-5b (WNT5B), ELISA Kit
Full Product Name
Human Protein Wnt-5b ELISA Kit
Product Gene Name
WNT5B elisa kit
Product Synonym Names
WNT5B (Protein Wnt-5b)
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.781-50ng/ml
Sensitivity
0.469ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
40,323 Da
OMIM
606361
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of WNT5B elisa kit

Human WNT5B ELISA Kit – Cat# MBS762196

Typical Standard Curve of WNT5B elisa kit

Human WNT5B ELISA Kit, Typical Standard Curve – Cat# MBS762196

Other Notes

Small volumes of WNT5B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WNT5B purchase

MBS762196 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein Wnt-5b (WNT5B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT5B. The ELISA analytical biochemical technique of the MBS762196 kit is based on WNT5B antibody-WNT5B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT5B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT5B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for WNT5B. It may not necessarily be applicable to this product.

NCBI GI #
17402919
NCBI GeneID
81029
NCBI Accession #
NP_110402.2
UniProt Secondary Accession #
Q96S49; Q9BV04; A8K315; D3DUP9
UniProt Related Accession #
Q9H1J7
GenBank Nucleotide #
NM_030775.2
NCBI Official Full Name
protein Wnt-5b
NCBI Official Synonym Full Names
wingless-type MMTV integration site family member 5B
NCBI Official Symbol
WNT5B
UniProt Protein Name
Protein Wnt-5b
Protein Family
Protein
UniProt Gene Name
WNT5B
UniProt Entry Name
WNT5B_HUMAN
NCBI Protein Information
protein Wnt-5b

NCBI Summary for WNT5B

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]
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UniProt Comments for WNT5B

WNT5B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Interacts with PORCN. Belongs to the Wnt family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: cell surface; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane; proteinaceous extracellular matrix

Molecular Function: frizzled binding; receptor binding

Biological Process: cell fate commitment; chondrocyte differentiation; fat cell differentiation; neuron differentiation; positive regulation of cell migration; positive regulation of fat cell differentiation; Wnt receptor signaling pathway; wound healing
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Research Articles on WNT5B

1. Results demonstrate that the Wnt5B pathway may play an important role in atypical teratoid rhabdoid tumor biology.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with WNT5B elisa kit

 Reference Product  PubMed Publications
 FZD2 elisa kit  >14 publications with WNT5B and FZD2
 FZD1 elisa kit  >13 publications with WNT5B and FZD1
 FZD4 elisa kit  >11 publications with WNT5B and FZD4
 FZD7 elisa kit  >11 publications with WNT5B and FZD7
 FZD3 elisa kit  >9 publications with WNT5B and FZD3
 FZD5 elisa kit  >8 publications with WNT5B and FZD5
 LRP6 elisa kit  >6 publications with WNT5B and LRP6
 SHH elisa kit  >5 publications with WNT5B and SHH
 FZD8 elisa kit  >4 publications with WNT5B and FZD8
 WLS elisa kit  >2 publications with WNT5B and WLS

Pathways associated with WNT5B elisa kit

 Products by Pathway  Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Beta-catenin Independent WNT Signaling Pathway antibodies  Beta-catenin Independent WNT Signaling Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram

Diseases associated with WNT5B elisa kit

 Disease Name  Pubmed Publications
 Congenital Abnormalities Antibodies  >8 publications with WNT5B and Congenital Abnormalities
 Disease Models, Animal Antibodies  >7 publications with WNT5B and Disease Models, Animal
 Inflammation Antibodies  >7 publications with WNT5B and Inflammation
 Nervous System Diseases Antibodies  >5 publications with WNT5B and Nervous System Diseases
 Lung Neoplasms Antibodies  >5 publications with WNT5B and Lung Neoplasms
 Necrosis Antibodies  >4 publications with WNT5B and Necrosis
 Kidney Diseases Antibodies  >4 publications with WNT5B and Kidney Diseases
 Liver Diseases Antibodies  >4 publications with WNT5B and Liver Diseases
 Fibrosis Antibodies  >4 publications with WNT5B and Fibrosis
 Lung Diseases Antibodies  >3 publications with WNT5B and Lung Diseases

Organs/Tissues associated with WNT5B elisa kit

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >24 publications with WNT5B and Bone
 Embryonic Tissue Antibodies  >21 publications with WNT5B and Embryonic Tissue
 Muscle Antibodies  >15 publications with WNT5B and Muscle
 Brain Antibodies  >12 publications with WNT5B and Brain
 Lung Antibodies  >11 publications with WNT5B and Lung
 Kidney Antibodies  >10 publications with WNT5B and Kidney
 Liver Antibodies  >9 publications with WNT5B and Liver
 Eye Antibodies  >9 publications with WNT5B and Eye
 Heart Antibodies  >9 publications with WNT5B and Heart
 Intestine Antibodies  >6 publications with WNT5B and Intestine
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