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Nodal Homolog (NODAL) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS762322

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-NODAL antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-NODAL antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Nodal homolog, ELISA Kit
Full Product Name
Human Nodal homolog ELISA Kit
Product Gene Name
NODAL elisa kit
Product Synonym Names
NODAL/Nodal homolog
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
78.125-5000pg/ml
Sensitivity
46.875pg/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
39,561 Da
OMIM
270100
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of NODAL elisa kit

Human NODAL ELISA Kit – Cat# MBS762322

Typical Standard Curve of NODAL elisa kit

Human NODAL ELISA Kit, Typical Standard Curve – Cat# MBS762322

Other Notes

Small volumes of NODAL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NODAL purchase

MBS762322 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nodal homolog, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NODAL. The ELISA analytical biochemical technique of the MBS762322 kit is based on NODAL antibody-NODAL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NODAL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NODAL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NODAL. It may not necessarily be applicable to this product.

NCBI GI #
222352098
NCBI GeneID
4838
NCBI Accession #
NP_060525.3
UniProt Secondary Accession #
Q2M3A5; Q8N4V3
UniProt Related Accession #
Q96S42
GenBank Nucleotide #
NM_018055.4
NCBI Official Full Name
nodal homolog
NCBI Official Synonym Full Names
nodal growth differentiation factor
NCBI Official Symbol
NODAL
NCBI Official Synonym Symbols
HTX5
UniProt Protein Name
Nodal homolog
Protein Family
Nodal
UniProt Gene Name
NODAL
UniProt Entry Name
NODAL_HUMAN
NCBI Protein Information
nodal homolog

NCBI Summary for NODAL

The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development. [provided by RefSeq, Jul 2008]

UniProt Comments for NODAL

NODAL: Essential for mesoderm formation and axial patterning during embryonic development. Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5). A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted; Cell development/differentiation; Motility/polarity/chemotaxis; Cytokine

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: extracellular space

Molecular Function: cytokine activity; growth factor activity; morphogen activity; receptor agonist activity; transforming growth factor beta receptor binding

Biological Process: axial mesodermal cell fate specification; BMP signaling pathway; brain development; cell development; cell migration involved in gastrulation; digestive tract morphogenesis; embryonic cranial skeleton morphogenesis; embryonic pattern specification; embryonic placenta development; embryonic process involved in female pregnancy; floor plate morphogenesis; formation of anatomical boundary; heart looping; liver development; maternal placenta development; maternal process involved in parturition; mesendoderm development; negative regulation of transcription from RNA polymerase II promoter; neural fold formation; placenta development; polarity specification of proximal/distal axis; positive regulation of activin receptor signaling pathway; positive regulation of angiogenesis; positive regulation of caspase activity; positive regulation of cell-cell adhesion; positive regulation of epithelial cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; regulation of gastrulation; repression of premature neural plate formation; stem cell maintenance; trophectodermal cellular morphogenesis; vasculature development

Disease: Heterotaxy, Visceral, 5, Autosomal
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Research Articles on NODAL

1. Nodal signaling pathway has subtle changes in the endometrium of women with endometriosis, but this imbalance may not cause functional damage as it seems not to affect the nuclear expression of SMAD4.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NODAL elisa kit

 Reference Product  PubMed Publications
 SMAD2 elisa kit  >204 publications with NODAL and SMAD2
 TDGF1 elisa kit  >93 publications with NODAL and TDGF1
 LEFTY1 elisa kit  >80 publications with NODAL and LEFTY1
 LEFTY2 elisa kit  >49 publications with NODAL and LEFTY2
 ACVR1B elisa kit  >43 publications with NODAL and ACVR1B
 ACVR1C elisa kit  >29 publications with NODAL and ACVR1C
 CFC1 elisa kit  >24 publications with NODAL and CFC1
 CER1 elisa kit  >17 publications with NODAL and CER1
 ACVR2B elisa kit  >9 publications with NODAL and ACVR2B
 ACVR2A elisa kit  >7 publications with NODAL and ACVR2A

Pathways associated with NODAL elisa kit

 Products by Pathway  Pathway Diagram
 Cardiac Progenitor Differentiation Pathway antibodies  Cardiac Progenitor Differentiation Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Regulation Of Signaling By NODAL Pathway antibodies  Regulation Of Signaling By NODAL Pathway Diagram
 Signaling By NODAL Pathway antibodies  Signaling By NODAL Pathway Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 Signaling Pathways Regulating Pluripotency Of Stem Cells antibodies  Signaling Pathways Regulating Pluripotency Of Stem Cells Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram

Diseases associated with NODAL elisa kit

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >11969 publications with NODAL and Carcinoma
 Neoplasm Metastasis Antibodies  >10820 publications with NODAL and Neoplasm Metastasis
 Cardiovascular Diseases Antibodies  >5862 publications with NODAL and Cardiovascular Diseases
 Heart Diseases Antibodies  >5613 publications with NODAL and Heart Diseases
 Arrhythmias, Cardiac Antibodies  >5319 publications with NODAL and Arrhythmias, Cardiac
 Breast Neoplasms Antibodies  >4603 publications with NODAL and Breast Neoplasms
 Adenocarcinoma Antibodies  >4543 publications with NODAL and Adenocarcinoma
 Lung Diseases Antibodies  >2263 publications with NODAL and Lung Diseases
 Nervous System Diseases Antibodies  >977 publications with NODAL and Nervous System Diseases
 Liver Neoplasms Antibodies  >793 publications with NODAL and Liver Neoplasms

Organs/Tissues associated with NODAL elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >990 publications with NODAL and Brain
 Embryonic Tissue Antibodies  >498 publications with NODAL and Embryonic Tissue
 Connective Tissue Antibodies  >215 publications with NODAL and Connective Tissue
 Testis Antibodies  >152 publications with NODAL and Testis
 Placenta Antibodies  >52 publications with NODAL and Placenta
 Uncharacterized Tissue Antibodies  >4 publications with NODAL and Uncharacterized Tissue
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