N-Terminal Propeptide of Collagen Alpha-1(ii) Chain (PIINP) ELISA Kit for Human

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Human PIINP. During the reaction,Human PIINP in the sample or standard competes with a fixed amount of Human PIINP on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Human PIINP. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human PIINP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Small volumes of PIINP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS762448 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the N-terminal propeptide of Collagen alpha-1(II) chain (PIINP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PIINP. The ELISA analytical biochemical technique of the MBS762448 kit is based on PIINP antibody-PIINP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PIINP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PIINP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).