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Delta-1-Pyrroline-5-Carboxylate Dehydrogenase, Mitochondrial (ALDH4A1) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS762517

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ALDH4A1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ALDH4A1 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial (ALDH4A1), ELISA Kit
Full Product Name
Human Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial ELISA Kit
Product Gene Name
ALDH4A1 elisa kit
Product Synonym Names
ALDH4A1/Delta-1-pyrroline-5-carboxylate dehydrogenase; mitochondrial/P5C dehydrogenase/Aldehyde dehydrogenase family 4 member A1/L-glutamate gamma-semialdehyde dehydrogenase/ALDH4/P5CDH/P5CD/P5CDh/P5CDhL/P5CDhS
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
56,043 Da
OMIM
239510
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of ALDH4A1 elisa kit

Human ALDH4A1 ELISA Kit – Cat# MBS762517

Typical Standard Curve of ALDH4A1 elisa kit

Human ALDH4A1 ELISA Kit, Typical Standard Curve – Cat# MBS762517

Other Notes

Small volumes of ALDH4A1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ALDH4A1 purchase

MBS762517 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial (ALDH4A1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ALDH4A1. The ELISA analytical biochemical technique of the MBS762517 kit is based on ALDH4A1 antibody-ALDH4A1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ALDH4A1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ALDH4A1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ALDH4A1. It may not necessarily be applicable to this product.

NCBI GI #
25777736
NCBI GeneID
8659
NCBI Accession #
NP_733844.1
UniProt Secondary Accession #
Q16882; Q53HU4; Q5JNV6; Q8IZ38; Q96IF0; Q9UDI6; A8K1Q7; B4DGE4; D2D4A3
UniProt Related Accession #
P30038
GenBank Nucleotide #
NM_170726.2
NCBI Official Full Name
delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial isoform a
NCBI Official Synonym Full Names
aldehyde dehydrogenase 4 family member A1
NCBI Official Symbol
ALDH4A1
NCBI Official Synonym Symbols
P5CD; ALDH4; P5CDh
UniProt Protein Name
Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
UniProt Synonym Protein Names
Aldehyde dehydrogenase family 4 member A1; L-glutamate gamma-semialdehyde dehydrogenase
UniProt Gene Name
ALDH4A1
UniProt Synonym Gene Names
ALDH4; P5CDH; P5C dehydrogenase
UniProt Entry Name
AL4A1_HUMAN
NCBI Protein Information
delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial

NCBI Summary for ALDH4A1

This protein belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2009]
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UniProt Comments for ALDH4A1

ALDH4A1: Irreversible conversion of delta-1-pyrroline-5- carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes. Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2). HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation. Belongs to the aldehyde dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; Oxidoreductase; EC 1.2.1.88; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: mitochondrial matrix

Molecular Function: 1-pyrroline-5-carboxylate dehydrogenase activity; aldehyde dehydrogenase (NAD) activity; electron carrier activity; identical protein binding

Biological Process: 4-hydroxyproline catabolic process; glutamate biosynthetic process; proline biosynthetic process; proline catabolic process; proline catabolic process to glutamate; proline metabolic process

Disease: Hyperprolinemia, Type Ii
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Research Articles on ALDH4A1

1. the impact of the hyperprolinemia-associated mutation of Ser352 to Leu on the structure and catalytic properties of the P5CDH

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ALDH4A1 elisa kit

 Reference Product  PubMed Publications
 ALDH18A1 elisa kit  >1 publications with ALDH4A1 and ALDH18A1

Pathways associated with ALDH4A1 elisa kit

 Products by Pathway  Pathway Diagram
 4-hydroxyproline Degradation Pathway antibodies  4-hydroxyproline Degradation Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Histidine, Lysine, Phenylalanine, Tyrosine, Proline And Tryptophan Catabolism Pathway antibodies  Histidine, Lysine, Phenylalanine, Tyrosine, Proline And Tryptophan Catabolism Pathway Diagram
 L-arginine Degradation I (arginase Pathway) antibodies  L-arginine Degradation I (arginase Pathway) Diagram
 L-proline Degradation Pathway antibodies  L-proline Degradation Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram

Diseases associated with ALDH4A1 elisa kit

 Disease Name  Pubmed Publications
 Hyperprolinemia type 2 Antibodies  >2 publications with ALDH4A1 and Hyperprolinemia type 2
 Cardiovascular Diseases Antibodies  >1 publications with ALDH4A1 and Cardiovascular Diseases
 Cell Transformation, Neoplastic Antibodies  >1 publications with ALDH4A1 and Cell Transformation, Neoplastic
 Fatty Liver Antibodies  >1 publications with ALDH4A1 and Fatty Liver
 Liver Neoplasms Antibodies  >1 publications with ALDH4A1 and Liver Neoplasms

Organs/Tissues associated with ALDH4A1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with ALDH4A1 and Blood
 Skin Antibodies  >4 publications with ALDH4A1 and Skin
 Liver Antibodies  >4 publications with ALDH4A1 and Liver
 Stomach Antibodies  >1 publications with ALDH4A1 and Stomach
 Muscle Antibodies  >1 publications with ALDH4A1 and Muscle
 Lung Antibodies  >1 publications with ALDH4A1 and Lung
 Embryonic Tissue Antibodies  >1 publications with ALDH4A1 and Embryonic Tissue
 Mouth Antibodies  >1 publications with ALDH4A1 and Mouth
 Placenta Antibodies  >1 publications with ALDH4A1 and Placenta
 Ovary Antibodies  >1 publications with ALDH4A1 and Ovary
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