Heparin-Binding Epidermal Growth Factor-Like Growth Factor (HB-EGF) ELISA Kit for Mouse

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse HB-EGF antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse HB-EGF antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse HB-EGF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse HB-EGF can be calculated.

Small volumes of HB-EGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS762599 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Heparin-binding Epidermal Growth Factor-like Growth Factor (HB-EGF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HB-EGF. The ELISA analytical biochemical technique of the MBS762599 kit is based on HB-EGF antibody-HB-EGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HB-EGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HB-EGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).