Rat ADRbetaK1 ELISA Kit – Cat# MBS762602

Adrenergic Receptor Beta Kinase 1 (ADRbetaK1) ELISA Kit for Rat

Name: Rat ADRbetaK1 ELISA Kit; Adrenergic Receptor Beta Kinase 1; MyBioSource
Brand: MyBioSource
SKU: MBS762602
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS762602

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ADRbeta K1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ADRbetaK1 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Adrenergic Receptor Beta Kinase 1 (ADRbetaK1), ELISA Kit

Full Product Name

Rat Adrenergic Receptor Beta Kinase 1 ELISA Kit

Product Gene Name

ADRbetaK1 elisa kit

Product Synonym Gene Name

ADRbK1

Product Synonym Names

ADRbK1; Adrenergic Receptor Beta Kinase 1

Species Reactivity

Rat

Assay Type

Sandwich ELISA, Double Antibody

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

0.156-10ng/ml

Sensitivity

0.094ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of ADRbetaK1. No significant cross-reactivity or interference between ADRbetaK1 and analogues was observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADR betaK1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADR betaK1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%

Molecular Weight

79,574 Da

OMIM

109635

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of ADRbetaK1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADRbetaK1 purchase

MBS762602 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Adrenergic Receptor Beta Kinase 1 (ADRbetaK1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ADRbetaK1. The ELISA analytical biochemical technique of the MBS762602 kit is based on ADRbetaK1 antibody-ADRbetaK1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ADRbetaK1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ADRbetaK1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ADRbetaK1. It may not necessarily be applicable to this product.

NCBI GI #

119594988

NCBI GeneID

156

NCBI Accession #

EAW74582.1

UniProt Secondary Accession #

Q13837; Q6GTT3; B0ZBE1

UniProt Related Accession #

P25098

NCBI Official Full Name

adrenergic, beta, receptor kinase 1, isoform CRA_b

NCBI Official Synonym Full Names

adrenergic, beta, receptor kinase 1

NCBI Official Symbol

ADRBK1

NCBI Official Synonym Symbols

GRK2; BARK1; BETA-ARK1

UniProt Protein Name

Beta-adrenergic receptor kinase 1

UniProt Synonym Protein Names

G-protein coupled receptor kinase 2

UniProt Gene Name

ADRBK1

UniProt Synonym Gene Names

BARK; BARK1; GRK2; Beta-ARK-1

UniProt Entry Name

ARBK1_HUMAN

NCBI Protein Information

beta-adrenergic receptor kinase 1

NCBI Summary for ADRbetaK1

The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008]

UniProt Comments for ADRbetaK1

GRK2: a ubiquitous protein kinase of the GRK family. Phosphorylates the beta-2-adrenergic receptor and related G-protein-coupled receptors. Appears to mediate agonist-specific desensitization observed at high agonist concentrations. Expression level is consistently elevated in chronic human heart failure. Mouse models of severe heart failure have been used to demonstrate that inhibition of BARK1 with a peptide inhibitor is sufficient to increase mean survival, reduce dialation and improve cardiac function.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; EC 2.7.11.15; AGC group; GRK family; BARK subfamily

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: cytoplasm; cytosol; membrane; plasma membrane

Molecular Function: alpha-2A adrenergic receptor binding; ATP binding; beta-adrenergic receptor kinase activity; Edg-2 lysophosphatidic acid receptor binding; G-protein coupled receptor kinase activity; protein binding; protein kinase activity

Biological Process: acetylcholine receptor signaling, muscarinic pathway; cardiac muscle contraction; desensitization of G-protein coupled receptor protein signaling pathway; entry of virus into host cell; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; heart development; innate immune response; negative regulation of striated muscle contraction; negative regulation of the force of heart contraction by chemical signal; nerve growth factor receptor signaling pathway; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phospholipase C activation; positive regulation of catecholamine secretion; receptor internalization; signal transduction; tachykinin signaling pathway; viral genome replication

Research Articles on ADRbetaK1

1. Acute aerobic exercise induces a greater GRK2 expression in women than men, while increased cardiorespiratory fitness is associated with exercise-induced GRK2 expression in PBMCs

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ADRbetaK1 elisa kit

Reference Product	PubMed Publications
ADRB2 elisa kit	>62 publications with ADRbetaK1 and ADRB2
CCR5 elisa kit	>2 publications with ADRbetaK1 and CCR5
OPN4 elisa kit	>1 publications with ADRbetaK1 and OPN4
FPR1 elisa kit	>1 publications with ADRbetaK1 and FPR1
GRM1 elisa kit	>1 publications with ADRbetaK1 and GRM1

Pathways associated with ADRbetaK1 elisa kit

Products by Pathway	Pathway Diagram
Activation Of SMO Pathway antibodies	Activation Of SMO Pathway Diagram
CXCR4-mediated Signaling Events Pathway antibodies	CXCR4-mediated Signaling Events Pathway Diagram
Ca-dependent Events Pathway antibodies	Ca-dependent Events Pathway Diagram
CaM Pathway antibodies	CaM Pathway Diagram
Calmodulin Induced Events Pathway antibodies	Calmodulin Induced Events Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
DAG And IP3 Signaling Pathway antibodies	DAG And IP3 Signaling Pathway Diagram
DAP12 Interactions Pathway antibodies	DAP12 Interactions Pathway Diagram
DAP12 Signaling Pathway antibodies	DAP12 Signaling Pathway Diagram

Diseases associated with ADRbetaK1 elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>148 publications with ADRbetaK1 and Cardiovascular Diseases
Heart Diseases Antibodies	>111 publications with ADRbetaK1 and Heart Diseases
Inflammation Antibodies	>62 publications with ADRbetaK1 and Inflammation
Nervous System Diseases Antibodies	>52 publications with ADRbetaK1 and Nervous System Diseases
Hypertension Antibodies	>52 publications with ADRbetaK1 and Hypertension
Hypertrophy Antibodies	>32 publications with ADRbetaK1 and Hypertrophy
Cardiomegaly Antibodies	>17 publications with ADRbetaK1 and Cardiomegaly
Necrosis Antibodies	>15 publications with ADRbetaK1 and Necrosis
Cardiomyopathies Antibodies	>14 publications with ADRbetaK1 and Cardiomyopathies
Postoperative Complications Antibodies	>14 publications with ADRbetaK1 and Postoperative Complications

Organs/Tissues associated with ADRbetaK1 elisa kit

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>141 publications with ADRbetaK1 and Muscle
Blood Antibodies	>130 publications with ADRbetaK1 and Blood
Brain Antibodies	>107 publications with ADRbetaK1 and Brain
Vascular Antibodies	>76 publications with ADRbetaK1 and Vascular
Kidney Antibodies	>71 publications with ADRbetaK1 and Kidney
Nerve Antibodies	>25 publications with ADRbetaK1 and Nerve
Lung Antibodies	>19 publications with ADRbetaK1 and Lung
Liver Antibodies	>16 publications with ADRbetaK1 and Liver
Bone Antibodies	>15 publications with ADRbetaK1 and Bone
Embryonic Tissue Antibodies	>14 publications with ADRbetaK1 and Embryonic Tissue