NEDD8-Activating Enzyme E1 Catalytic Subunit (UBA3) ELISA Kit for Human

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

UBA3: Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. Belongs to the ubiquitin-activating E1 family. UBA3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; EC 6.3.2.19; EC 6.3.2.-; Nuclear receptor co-regulator; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: cytosol; nucleus

Molecular Function: acid-amino acid ligase activity; ATP binding; ligand-dependent nuclear receptor binding; NEDD8 activating enzyme activity; protein binding; protein heterodimerization activity

Biological Process: endomitotic cell cycle; innate immune response; negative regulation of transcription, DNA-dependent; protein modification process; protein neddylation; proteolysis; regulation of cell cycle; stimulatory C-type lectin receptor signaling pathway; tumor necrosis factor-mediated signaling pathway