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Histo-Blood Group ABO System Transferase (ABO) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS762759

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-A2M antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-A2M antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Histo-blood group ABO system transferase, ELISA Kit
Full Product Name
Human Histo-blood group ABO system transferase ELISA Kit
Product Gene Name
ABO elisa kit
Product Synonym Names
ABO/Histo-blood group B transferase (B transferase)/Histo-blood group A transferase (A transferase)/Glycoprotein-fucosylgalactoside alpha-galactosyltransferase)/Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase)/NAGAT/Fucosylglycoprotein 3-alpha-galactosyltransferase/Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of A2M. No significant cross-reactivity or interference between A2M and analogues was observed.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A2M were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A2M were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Molecular Weight
40,934 Da
OMIM
110300
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of ABO elisa kit

Human ABO ELISA Kit – Cat# MBS762759

Other Notes

Small volumes of ABO elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ABO purchase

MBS762759 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Histo-blood group ABO system transferase, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ABO. The ELISA analytical biochemical technique of the MBS762759 kit is based on ABO antibody-ABO antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ABO antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ABO. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ABO. It may not necessarily be applicable to this product.

NCBI GI #
58331216
NCBI GeneID
28
NCBI Accession #
NP_065202.2
UniProt Secondary Accession #
O14758; Q14490; Q53I57; Q6ISD4; Q6KFZ2; Q70V27; Q99484; Q99485; Q9NY01; Q9UQ68; B0JDB9
UniProt Related Accession #
P16442
GenBank Nucleotide #
NM_020469.2
NCBI Official Full Name
histo-blood group ABO system transferase
NCBI Official Synonym Full Names
ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase)
NCBI Official Symbol
ABO
NCBI Official Synonym Symbols
GTB; NAGAT; A3GALNT; A3GALT1
UniProt Protein Name
Histo-blood group ABO system transferase
UniProt Synonym Protein Names
Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase (EC:2.4.1.40
Protein Family
4-methylaminobutanoate oxidase
UniProt Gene Name
ABO
UniProt Synonym Gene Names
A transferase; B transferase
UniProt Entry Name
BGAT_HUMAN
NCBI Protein Information
histo-blood group ABO system transferase

NCBI Summary for ABO

This gene encodes proteins related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The 'O' blood group is caused by a deletion of guanine-258 near the N-terminus of the protein which results in a frameshift and translation of an almost entirely different protein. Individuals with the A, B, and AB alleles express glycosyltransferase activities that convert the H antigen into the A or B antigen. Other minor alleles have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for ABO

ABO: This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. Belongs to the glycosyltransferase 6 family.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Membrane protein, integral; EC 2.4.1.37; Transferase; EC 2.4.1.40

Chromosomal Location of Human Ortholog: 9q34.2

Cellular Component: extracellular region; integral to membrane

Molecular Function: fucosylgalactoside 3-alpha-galactosyltransferase activity; glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase activity; metal ion binding

Biological Process: protein amino acid glycosylation

Disease: Blood Group, Abo System
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Research Articles on ABO

1. Non-O blood type is associated with future cardiovascular events in Chinese Han patients undergoing coronary angiography.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with ABO elisa kit

 Products by Pathway  Pathway Diagram
 Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway antibodies  Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway Diagram
 Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway antibodies  Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram

Diseases associated with ABO elisa kit

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >993 publications with ABO and Cardiovascular Diseases
 Vascular Diseases Antibodies  >780 publications with ABO and Vascular Diseases
 Kidney Diseases Antibodies  >486 publications with ABO and Kidney Diseases
 Inflammation Antibodies  >304 publications with ABO and Inflammation
 Diabetes Mellitus Antibodies  >259 publications with ABO and Diabetes Mellitus
 Urinary Bladder Neoplasms Antibodies  >224 publications with ABO and Urinary Bladder Neoplasms
 Hypertension Antibodies  >197 publications with ABO and Hypertension
 Necrosis Antibodies  >183 publications with ABO and Necrosis
 Liver Neoplasms Antibodies  >169 publications with ABO and Liver Neoplasms
 Duodenal Ulcer Antibodies  >160 publications with ABO and Duodenal Ulcer

Organs/Tissues associated with ABO elisa kit

 Organ/Tissue Name  Pubmed Publications
 Eye Antibodies  >1050 publications with ABO and Eye
 Bone Marrow Antibodies  >645 publications with ABO and Bone Marrow
 Lung Antibodies  >428 publications with ABO and Lung
 Intestine Antibodies  >363 publications with ABO and Intestine
 Mouth Antibodies  >276 publications with ABO and Mouth
 Bladder Antibodies  >274 publications with ABO and Bladder
 Pancreas Antibodies  >160 publications with ABO and Pancreas
 Salivary Gland Antibodies  >119 publications with ABO and Salivary Gland
 Esophagus Antibodies  >41 publications with ABO and Esophagus
 Testis Antibodies  >40 publications with ABO and Testis
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