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Neuromodulin (GAP43) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS763002

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human GAP43 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human GAP43 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Neuromodulin (GAP43), ELISA Kit
Full Product Name
Human Neuromodulin ELISA Kit
Product Gene Name
GAP43 elisa kit
Product Synonym Names
GAP43 (Growth Associated Protein 43)/B-50/Basp2/PP46/Neuromodulin/Axonal membrane protein GAP-43/calmodulin-binding protein P-57/nerve growth-related peptide GAP43/Neural phosphoprotein B-50/neuron growth-associated protein 43/Growth-associated protein 43
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
28,766 Da
OMIM
162060
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of GAP43 elisa kit

Human GAP43 ELISA Kit – Cat# MBS763002

Typical Standard Curve of GAP43 elisa kit

Human GAP43 ELISA Kit, Typical Standard Curve – Cat# MBS763002

Other Notes

Small volumes of GAP43 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GAP43 purchase

MBS763002 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Neuromodulin (GAP43) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GAP43. The ELISA analytical biochemical technique of the MBS763002 kit is based on GAP43 antibody-GAP43 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GAP43 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GAP43. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GAP43. It may not necessarily be applicable to this product.

NCBI GI #
4503911
NCBI GeneID
2596
NCBI Accession #
NP_002036.1
UniProt Secondary Accession #
A8K0Y4
UniProt Related Accession #
P17677
GenBank Nucleotide #
NM_002045.3
NCBI Official Full Name
neuromodulin isoform 2
NCBI Official Synonym Full Names
growth associated protein 43
NCBI Official Symbol
GAP43
NCBI Official Synonym Symbols
B-50; PP46
UniProt Protein Name
Neuromodulin
UniProt Synonym Protein Names
Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46
Protein Family
Neuromodulin
UniProt Gene Name
GAP43
UniProt Entry Name
NEUM_HUMAN
NCBI Protein Information
neuromodulin

NCBI Summary for GAP43

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for GAP43

GAP43: This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence. Belongs to the neuromodulin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface

Chromosomal Location of Human Ortholog: 3q13.31

Cellular Component: axon; cell junction; cytoplasm; filopodium membrane; plasma membrane; postsynaptic density

Molecular Function: calmodulin binding; phosphatidylserine binding; protein binding

Biological Process: axon choice point recognition; cell fate commitment; glial cell differentiation; protein kinase C activation; regulation of filopodium formation; regulation of growth; response to wounding; tissue regeneration
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Research Articles on GAP43

1. Results show that PKC-dependent phosphorylation of GAP43 plays a critical role in regulating postsynaptic gephyrin aggregation in developing GABAergic synapses.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GAP43 elisa kit

 Reference Product  PubMed Publications
 BDNF elisa kit  >35 publications with GAP43 and BDNF
 MAP2 elisa kit  >24 publications with GAP43 and MAP2
 POMC elisa kit  >6 publications with GAP43 and POMC
 BASP1 elisa kit  >4 publications with GAP43 and BASP1
 NRGN elisa kit  >4 publications with GAP43 and NRGN
 ELAVL4 elisa kit  >3 publications with GAP43 and ELAVL4

Pathways associated with GAP43 elisa kit

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 Glial Cell Differentiation Pathway antibodies  Glial Cell Differentiation Pathway Diagram
 N-cadherin Signaling Events Pathway antibodies  N-cadherin Signaling Events Pathway Diagram
 Spinal Cord Injury Pathway antibodies  Spinal Cord Injury Pathway Diagram

Diseases associated with GAP43 elisa kit

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >150 publications with GAP43 and Nervous System Diseases
 Brain Diseases Antibodies  >63 publications with GAP43 and Brain Diseases
 Cardiovascular Diseases Antibodies  >52 publications with GAP43 and Cardiovascular Diseases
 Heart Diseases Antibodies  >36 publications with GAP43 and Heart Diseases
 Inflammation Antibodies  >27 publications with GAP43 and Inflammation
 Brain Injuries Antibodies  >24 publications with GAP43 and Brain Injuries
 Nerve Degeneration Antibodies  >23 publications with GAP43 and Nerve Degeneration
 Arrhythmias, Cardiac Antibodies  >17 publications with GAP43 and Arrhythmias, Cardiac
 Necrosis Antibodies  >11 publications with GAP43 and Necrosis
 Learning Disorders Antibodies  >5 publications with GAP43 and Learning Disorders

Organs/Tissues associated with GAP43 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >293 publications with GAP43 and Nerve
 Brain Antibodies  >240 publications with GAP43 and Brain
 Eye Antibodies  >32 publications with GAP43 and Eye
 Embryonic Tissue Antibodies  >31 publications with GAP43 and Embryonic Tissue
 Bone Antibodies  >21 publications with GAP43 and Bone
 Vascular Antibodies  >19 publications with GAP43 and Vascular
 Connective Tissue Antibodies  >3 publications with GAP43 and Connective Tissue
 Lung Antibodies  >2 publications with GAP43 and Lung
 Mouth Antibodies  >1 publications with GAP43 and Mouth
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