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Protein Atonal Homolog 1 (ATOH1) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS763053

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ATOH1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ATOH1 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Protein atonal homolog 1 (ATOH1), ELISA Kit
Full Product Name
Human Protein atonal homolog 1 ELISA Kit
Product Gene Name
ATOH1 elisa kit
Product Synonym Names
ATOH1 (Protein atonal homolog 1)/ATH1/Class A basic helix-loop-helix protein 14 (bHLHa14)/Helix-loop-helix protein hATH-1 (hATH1)/BHLHA14
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.781-50ng/ml
Sensitivity
0.469ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
38,160 Da
OMIM
601461
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of ATOH1 elisa kit

Human ATOH1 ELISA Kit – Cat# MBS763053

Typical Standard Curve of ATOH1 elisa kit

Human ATOH1 ELISA Kit, Typical Standard Curve – Cat# MBS763053

Other Notes

Small volumes of ATOH1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATOH1 purchase

MBS763053 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein atonal homolog 1 (ATOH1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ATOH1. The ELISA analytical biochemical technique of the MBS763053 kit is based on ATOH1 antibody-ATOH1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ATOH1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ATOH1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ATOH1. It may not necessarily be applicable to this product.

NCBI GI #
4885075
NCBI GeneID
474
NCBI Accession #
NP_005163.1
UniProt Secondary Accession #
Q14CT9
UniProt Related Accession #
Q92858
GenBank Nucleotide #
NM_005172.1
NCBI Official Full Name
protein atonal homolog 1
NCBI Official Synonym Full Names
atonal bHLH transcription factor 1
NCBI Official Symbol
ATOH1
NCBI Official Synonym Symbols
ATH1; HATH1; MATH-1; bHLHa14
UniProt Protein Name
Protein atonal homolog 1
UniProt Synonym Protein Names
Class A basic helix-loop-helix protein 14; bHLHa14; Helix-loop-helix protein hATH-1; hATH1
Protein Family
Protein atonal
UniProt Gene Name
ATOH1
UniProt Synonym Gene Names
ATH1; BHLHA14; bHLHa14; hATH1
UniProt Entry Name
ATOH1_HUMAN
NCBI Protein Information
protein atonal homolog 1

NCBI Summary for ATOH1

This protein belongs to the basic helix-loop-helix (BHLH) family of transcription factors. It activates E-box dependent transcription along with E47. [provided by RefSeq, Jul 2008]

UniProt Comments for ATOH1

ATOH1: a conserved transcription factor that regulates secretory cell differentiation in colonic epithelium. Activates E box-dependent transcription in collaboration with TCF3/E47, but the activity is completely antagonized by the negative regulator of neurogenesis HES1. May play a role in the differentiation of subsets of neural cells by activating E box- dependent transcription . Efficient DNA binding requires dimerization with another bHLH protein. Loss of ATOH1 expression in the colon of mice initiates colon cancer. ATOH1 has been silenced in most human colon cancers, and reactivation of this gene in cultured human colon cancer cells is anti-proliferative and induces apoptosis.

Protein type: Transcription factor; DNA-binding; Cell development/differentiation

Chromosomal Location of Human Ortholog: 4q22

Cellular Component: nucleus

Molecular Function: chromatin DNA binding; protein dimerization activity; transcription factor activity

Biological Process: auditory receptor cell fate determination; auditory receptor cell fate specification; axon guidance; central nervous system development; cerebral cortex development; inner ear morphogenesis; negative regulation of apoptosis; neuron migration; Notch signaling pathway; positive regulation of auditory receptor cell differentiation; positive regulation of neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
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Research Articles on ATOH1

1. Atoh1 epigenetic regulation guides hair cell development in the mammalian cochlea

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ATOH1 elisa kit

 Reference Product  PubMed Publications
 SOX2 elisa kit  >30 publications with ATOH1 and SOX2
 SHH elisa kit  >21 publications with ATOH1 and SHH
 NOTCH1 elisa kit  >19 publications with ATOH1 and NOTCH1
 CTNNB1 elisa kit  >16 publications with ATOH1 and CTNNB1
 FGF8 elisa kit  >10 publications with ATOH1 and FGF8
 TCF4 elisa kit  >4 publications with ATOH1 and TCF4
 MUC5AC elisa kit  >3 publications with ATOH1 and MUC5AC
 ZIC1 elisa kit  >2 publications with ATOH1 and ZIC1
 TCF3 elisa kit  >1 publications with ATOH1 and TCF3
 TCF12 elisa kit  >1 publications with ATOH1 and TCF12

Diseases associated with ATOH1 elisa kit

 Disease Name  Pubmed Publications
 Medulloblastoma Antibodies  >35 publications with ATOH1 and Medulloblastoma
 Brain Diseases Antibodies  >21 publications with ATOH1 and Brain Diseases
 Disease Models, Animal Antibodies  >19 publications with ATOH1 and Disease Models, Animal
 Inflammation Antibodies  >10 publications with ATOH1 and Inflammation
 Necrosis Antibodies  >6 publications with ATOH1 and Necrosis
 Hyperplasia Antibodies  >5 publications with ATOH1 and Hyperplasia
 Lung Neoplasms Antibodies  >5 publications with ATOH1 and Lung Neoplasms
 Lung Diseases Antibodies  >3 publications with ATOH1 and Lung Diseases
 Nerve Degeneration Antibodies  >3 publications with ATOH1 and Nerve Degeneration
 Atrophy Antibodies  >1 publications with ATOH1 and Atrophy

Organs/Tissues associated with ATOH1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Mixed Antibodies  >1 publications with ATOH1 and Mixed
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