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Growth Hormone Releasing Hormone (GHRH) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS763140

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Ghrh antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Ghrh antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Growth Hormone Releasing Hormone (Ghrh), ELISA Kit
Full Product Name
Mouse Growth Hormone Releasing Hormone ELISA Kit
Product Gene Name
Ghrh elisa kit
Product Synonym Names
Ghrh/GRF/GHRF/Somatorelin/Somatocrinin/Growth hormone-releasing factor
Species Reactivity
Mouse
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
15.625-1000pg/ml
Sensitivity
9.375pg/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of Ghrh . No significant cross-reactivity or interference between Ghrh and analogues was observed.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ghrh were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ghrh were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Molecular Weight
47,402 Da
OMIM
139191
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of Ghrh elisa kit

Mouse GHRH ELISA Kit – Cat# MBS763140

Other Notes

Small volumes of Ghrh elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ghrh purchase

MBS763140 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Growth Hormone Releasing Hormone (Ghrh) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Ghrh. The ELISA analytical biochemical technique of the MBS763140 kit is based on Ghrh antibody-Ghrh antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Ghrh antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Ghrh. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Ghrh. It may not necessarily be applicable to this product.

NCBI GI #
1706945
NCBI GeneID
2692
NCBI Accession #
AAB37758.1
UniProt Secondary Accession #
Q99863
UniProt Related Accession #
Q02643
NCBI Official Full Name
growth hormone releasing hormone, partial
NCBI Official Synonym Full Names
growth hormone releasing hormone receptor
NCBI Official Symbol
GHRHR
NCBI Official Synonym Symbols
GRFR; GHRFR; IGHD1B
UniProt Protein Name
Growth hormone-releasing hormone receptor
UniProt Synonym Protein Names
Growth hormone-releasing factor receptor; GRF receptor; GRFR
Protein Family
Somatoliberin
UniProt Gene Name
GHRHR
UniProt Synonym Gene Names
GHRH receptor; GRF receptor; GRFR
UniProt Entry Name
GHRHR_HUMAN
NCBI Protein Information
growth hormone-releasing hormone receptor

NCBI Summary for Ghrh

This gene encodes a receptor for growth hormone-releasing hormone. Binding of this hormone to the receptor leads to synthesis and release of growth hormone. Mutations in this gene have been associated with isolated growth hormone deficiency (IGHD), also known as Dwarfism of Sindh, a disorder characterized by short stature. [provided by RefSeq, Jun 2010]
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UniProt Comments for Ghrh

GHRHR: Receptor for GRF, coupled to G proteins which activate adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone gene transcription and growth hormone secretion. Defects in GHRHR are a cause of growth hormone deficiency isolated type 1B (IGHD1B); also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Belongs to the G-protein coupled receptor 2 family.

Protein type: Receptor, GPCR; Cell development/differentiation; Membrane protein, integral; GPCR, family 2; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p14

Cellular Component: cell surface; cytoplasm; integral to membrane; nuclear inner membrane; nuclear matrix; nuclear outer membrane; plasma membrane; sarcolemma; secretory granule

Molecular Function: G-protein coupled receptor activity; growth factor binding; growth hormone-releasing hormone receptor activity; peptide hormone binding; protein binding

Biological Process: adenylate cyclase activation; cAMP-mediated signaling; cell maturation; cell surface receptor linked signal transduction; cellular response to insulin stimulus; determination of adult life span; G-protein signaling, adenylate cyclase activating pathway; growth hormone secretion; hormone metabolic process; lactation; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; positive regulation of circadian sleep/wake cycle, non-REM sleep; positive regulation of growth hormone secretion; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of multicellular organism growth; regulation of protein metabolic process; regulation of steroid hormone receptor signaling pathway; reproductive process in a multicellular organism; response to estrogen stimulus; response to glucocorticoid stimulus; response to insulin stimulus; somatotropin secreting cell development; water homeostasis

Disease: Isolated Growth Hormone Deficiency, Type Ib
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Research Articles on Ghrh

1. we found that pGHRH-R and its main splice variant are expressed in thyroid tissue and was upregulated in tumor cells compared to normal thyroid cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Ghrh elisa kit

 Reference Product  PubMed Publications
 GHRH elisa kit  >62 publications with Ghrh and GHRH
 POU1F1 elisa kit  >10 publications with Ghrh and POU1F1
 NPS elisa kit  >1 publications with Ghrh and NPS
 PTH elisa kit  >1 publications with Ghrh and PTH
 VIP elisa kit  >1 publications with Ghrh and VIP
 GNAS elisa kit  >1 publications with Ghrh and GNAS

Pathways associated with Ghrh elisa kit

 Products by Pathway  Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 G Alpha (s) Signalling Events Pathway antibodies  G Alpha (s) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 GPCRs, Class B Secretin-like Pathway antibodies  GPCRs, Class B Secretin-like Pathway Diagram
 GPCRs, Other Pathway antibodies  GPCRs, Other Pathway Diagram
 Glucagon-type Ligand Receptors Pathway antibodies  Glucagon-type Ligand Receptors Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Neuroactive Ligand-receptor Interaction Pathway antibodies  Neuroactive Ligand-receptor Interaction Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram

Diseases associated with Ghrh elisa kit

 Disease Name  Pubmed Publications
 Isolated Growth Hormone Deficiency, Type IB Antibodies  >13 publications with Ghrh and Isolated Growth Hormone Deficiency, Type IB
 Disease Models, Animal Antibodies  >5 publications with Ghrh and Disease Models, Animal
 Breast Neoplasms Antibodies  >4 publications with Ghrh and Breast Neoplasms
 Weight Loss Antibodies  >3 publications with Ghrh and Weight Loss
 Cardiovascular Diseases Antibodies  >2 publications with Ghrh and Cardiovascular Diseases
 Osteoporosis Antibodies  >1 publications with Ghrh and Osteoporosis
 Liver Diseases Antibodies  >1 publications with Ghrh and Liver Diseases
 Weight Gain Antibodies  >1 publications with Ghrh and Weight Gain
 Prostatic Diseases Antibodies  >1 publications with Ghrh and Prostatic Diseases
 Necrosis Antibodies  >1 publications with Ghrh and Necrosis
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