Dickkopf-Related Protein 1 (DKK-1) ELISA Kit for Human

Name: Human DKK-1 ELISA Kit; Dickkopf-Related Protein 1; MyBioSource
Brand: MyBioSource
SKU: MBS763163
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS763163

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-dectin 1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-dectin 1 antibody was used as detection antibodies. The standards,… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Dickkopf-related protein 1 (DKK-1), ELISA Kit

Full Product Name

Human Dickkopf-related protein 1 ELISA Kit

Product Gene Name

DKK-1 elisa kit

Product Synonym Names

DKK1/dickkopf (Xenopus laevis) homolog 1/dickkopf homolog 1 (Xenopus laevis)/dickkopf related protein-1/Dickkopf-1/dickkopf-related protein 1/Dkk-1/DKK-1/SKdickkopf-1 like

Species Reactivity

Human

Assay Type

Sandwich ELISA, Double Antibody

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

31.25-2000pg/ml

Sensitivity

18.75pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of dectin 1. No significant cross-reactivity or interference between dectin 1 and analogues was observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level dectin 1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level dectin 1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%

Molecular Weight

28,672 Da

OMIM

605189

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of DKK-1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DKK-1 purchase

MBS763163 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dickkopf-related protein 1 (DKK-1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DKK-1. The ELISA analytical biochemical technique of the MBS763163 kit is based on DKK-1 antibody-DKK-1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DKK-1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DKK-1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DKK-1. It may not necessarily be applicable to this product.

NCBI GI #

7110719

NCBI GeneID

22943

NCBI Accession #

NP_036374.1

UniProt Secondary Accession #

B2RC19

UniProt Related Accession #

O94907

GenBank Nucleotide #

NM_012242.2

NCBI Official Full Name

dickkopf-related protein 1

NCBI Official Synonym Full Names

dickkopf WNT signaling pathway inhibitor 1

NCBI Official Symbol

DKK1

NCBI Official Synonym Symbols

SK; DKK-1

UniProt Protein Name

Dickkopf-related protein 1

UniProt Synonym Protein Names

Protein Family

Dickkopf-related protein

UniProt Gene Name

DKK1

UniProt Synonym Gene Names

Dickkopf-1; Dkk-1; hDkk-1

UniProt Entry Name

DKK1_HUMAN

NCBI Protein Information

dickkopf-related protein 1

NCBI Summary for DKK-1

This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq, Jul 2008]

UniProt Comments for DKK-1

DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: early endosome membrane; extracellular region; extracellular space; plasma membrane

Molecular Function: growth factor activity; low-density lipoprotein receptor binding; protein binding; receptor antagonist activity; signal transducer activity

Biological Process: cellular morphogenesis during differentiation; embryonic limb morphogenesis; endoderm formation; forebrain development; hair follicle development; mesoderm formation; negative regulation of BMP signaling pathway; negative regulation of mesodermal cell fate specification; negative regulation of peptidyl-serine phosphorylation; negative regulation of protein binding; negative regulation of skeletal muscle development; negative regulation of synaptogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of Wnt receptor signaling pathway; regulation of endodermal cell fate specification; regulation of receptor internalization; response to retinoic acid

Research Articles on DKK-1

1. Our results demonstrated that the Wnt pathway inhibitor DKK-1 was decreased in CLL. OPN was increased in NHL and associated with extranodal involvement.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DKK-1 elisa kit

Reference Product	PubMed Publications
CTNNB1 elisa kit	>568 publications with DKK-1 and CTNNB1
WNT3A elisa kit	>146 publications with DKK-1 and WNT3A
LRP5 elisa kit	>125 publications with DKK-1 and LRP5
LRP6 elisa kit	>113 publications with DKK-1 and LRP6
MYC elisa kit	>58 publications with DKK-1 and MYC
WNT1 elisa kit	>44 publications with DKK-1 and WNT1
KREMEN1 elisa kit	>19 publications with DKK-1 and KREMEN1
KREMEN2 elisa kit	>15 publications with DKK-1 and KREMEN2
TP53 elisa kit	>10 publications with DKK-1 and TP53
TCF7L2 elisa kit	>5 publications with DKK-1 and TCF7L2

Pathways associated with DKK-1 elisa kit

Products by Pathway	Pathway Diagram
Cardiac Progenitor Differentiation Pathway antibodies	Cardiac Progenitor Differentiation Pathway Diagram
Direct P53 Effectors Pathway antibodies	Direct P53 Effectors Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram
Diseases Of Signal Transduction Pathway antibodies	Diseases Of Signal Transduction Pathway Diagram
Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway antibodies	Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway Diagram
Negative Regulation Of TCF-dependent Signaling By WNT Ligand Antagonists Pathway antibodies	Negative Regulation Of TCF-dependent Signaling By WNT Ligand Antagonists Pathway Diagram
Presenilin Action In Notch And Wnt Signaling Pathway antibodies	Presenilin Action In Notch And Wnt Signaling Pathway Diagram
Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies	Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram
Signaling By WNT In Cancer Pathway antibodies	Signaling By WNT In Cancer Pathway Diagram

Diseases associated with DKK-1 elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>102 publications with DKK-1 and Cardiovascular Diseases
Inflammation Antibodies	>61 publications with DKK-1 and Inflammation
Nervous System Diseases Antibodies	>48 publications with DKK-1 and Nervous System Diseases
Necrosis Antibodies	>43 publications with DKK-1 and Necrosis
Brain Diseases Antibodies	>43 publications with DKK-1 and Brain Diseases
Fractures, Bone Antibodies	>43 publications with DKK-1 and Fractures, Bone
Liver Diseases Antibodies	>42 publications with DKK-1 and Liver Diseases
Breast Neoplasms Antibodies	>40 publications with DKK-1 and Breast Neoplasms
Kidney Diseases Antibodies	>36 publications with DKK-1 and Kidney Diseases
Neoplasm Metastasis Antibodies	>34 publications with DKK-1 and Neoplasm Metastasis

Organs/Tissues associated with DKK-1 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>549 publications with DKK-1 and Bone
Bone Marrow Antibodies	>108 publications with DKK-1 and Bone Marrow
Embryonic Tissue Antibodies	>106 publications with DKK-1 and Embryonic Tissue
Connective Tissue Antibodies	>91 publications with DKK-1 and Connective Tissue
Brain Antibodies	>87 publications with DKK-1 and Brain
Vascular Antibodies	>73 publications with DKK-1 and Vascular
Liver Antibodies	>62 publications with DKK-1 and Liver
Skin Antibodies	>53 publications with DKK-1 and Skin
Muscle Antibodies	>53 publications with DKK-1 and Muscle
Lung Antibodies	>49 publications with DKK-1 and Lung