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PC4 and SFRS1-Interacting (PSIP1) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS763190

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PSIP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PSIP1 antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PC4 and SFRS1-interacting protein (PSIP1), ELISA Kit
Full Product Name
Human PC4 and SFRS1-interacting protein ELISA Kit
Product Gene Name
PSIP1 elisa kit
Product Synonym Names
PSIP1/PC4 and SFRS1-interacting protein/Transcriptional coactivator p75/p52/Lens epithelium-derived growth factor/CLL-associated antigen KW-7/Dense fine speckles 70 kDa protein/DFS 70/DFS70/LEDGF/PSIP2
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
78.125-5000pg/ml
Sensitivity
46.875pg/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
37,268 Da
OMIM
603620
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of PSIP1 elisa kit

Human PSIP1 ELISA Kit – Cat# MBS763190

Typical Standard Curve of PSIP1 elisa kit

Human PSIP1 ELISA Kit, Typical Standard Curve – Cat# MBS763190

Other Notes

Small volumes of PSIP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PSIP1 purchase

MBS763190 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the PC4 and SFRS1-interacting protein (PSIP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PSIP1. The ELISA analytical biochemical technique of the MBS763190 kit is based on PSIP1 antibody-PSIP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PSIP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PSIP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PSIP1. It may not necessarily be applicable to this product.

NCBI GI #
190014588
NCBI GeneID
11168
NCBI Accession #
NP_001121689.1
UniProt Secondary Accession #
O00256; O95368; Q6P391; Q86YB9; Q9NZI3; Q9UER6; D3DRI9
UniProt Related Accession #
O75475
GenBank Nucleotide #
NM_001128217.2
NCBI Official Full Name
PC4 and SFRS1-interacting protein isoform 2
NCBI Official Synonym Full Names
PC4 and SFRS1 interacting protein 1
NCBI Official Symbol
PSIP1
NCBI Official Synonym Symbols
p52; p75; PAIP; DFS70; LEDGF; PSIP2
UniProt Protein Name
PC4 and SFRS1-interacting protein
UniProt Synonym Protein Names
CLL-associated antigen KW-7; Dense fine speckles 70 kDa protein; DFS 70; Lens epithelium-derived growth factor; Transcriptional coactivator p75/p52
Protein Family
Lens epithelium-derived growth factor
UniProt Gene Name
PSIP1
UniProt Synonym Gene Names
DFS70; LEDGF; PSIP2; DFS 70
UniProt Entry Name
PSIP1_HUMAN
NCBI Protein Information
PC4 and SFRS1-interacting protein

UniProt Comments for PSIP1

LEDGF: Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration. Monomer. Interacts with IFRD1/PC4. Isoform 2 interacts with SFRS1. Isoform 1 interacts POGZ and CDCA7L. Isoform 1 interacts with lentiviral integrase and acts as a chromatin tethering factor to the chromosomal DNA. Isoform 1 interacts in particular with the human HIV-1 integrase protein (HIV-1 IN), determining its nuclear localization, its tight association with chromatin and its protection from the proteasome. Isoform 1 interacts also with HIV-2 IN. Isoform 2 does not interact with HIV-1 IN. Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions. Belongs to the HDGF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 9p22.3

Cellular Component: cytosol; nuclear heterochromatin; nucleolus; nucleoplasm; nucleus

Molecular Function: chromatin binding; protein binding; transcription activator binding

Biological Process: nuclear mRNA 5'-splice site recognition; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to heat; response to oxidative stress; transcription, DNA-dependent; viral reproduction
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Research Articles on PSIP1

1. Together, these results provide evidence for an Integrase/Pol mediated uptake of LEDGF/p75 in HIV-1 viral particles and a specific cleavage by HIV protease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PSIP1 elisa kit

 Reference Product  PubMed Publications
 MLL elisa kit  >4 publications with PSIP1 and MLL
 MEN1 elisa kit  >4 publications with PSIP1 and MEN1
 SP1 elisa kit  >3 publications with PSIP1 and SP1
 RBBP8 elisa kit  >2 publications with PSIP1 and RBBP8
 PABPC1 elisa kit  >1 publications with PSIP1 and PABPC1
 HSPA2 elisa kit  >1 publications with PSIP1 and HSPA2
 SRSF1 elisa kit  >1 publications with PSIP1 and SRSF1

Pathways associated with PSIP1 elisa kit

 Products by Pathway  Pathway Diagram
 2-LTR Circle Formation Pathway antibodies  2-LTR Circle Formation Pathway Diagram
 APOBEC3G Mediated Resistance To HIV-1 Infection Pathway antibodies  APOBEC3G Mediated Resistance To HIV-1 Infection Pathway Diagram
 Autointegration Results In Viral DNA Circles Pathway antibodies  Autointegration Results In Viral DNA Circles Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Early Phase Of HIV Life Cycle Pathway antibodies  Early Phase Of HIV Life Cycle Pathway Diagram
 HIV Infection Pathway antibodies  HIV Infection Pathway Diagram
 HIV Life Cycle Pathway antibodies  HIV Life Cycle Pathway Diagram
 Host Interactions Of HIV Factors Pathway antibodies  Host Interactions Of HIV Factors Pathway Diagram
 Infectious Disease Pathway antibodies  Infectious Disease Pathway Diagram
 Integration Of Provirus Pathway antibodies  Integration Of Provirus Pathway Diagram

Diseases associated with PSIP1 elisa kit

 Disease Name  Pubmed Publications
 Leukemia, Myeloid, Acute Antibodies  >5 publications with PSIP1 and Leukemia, Myeloid, Acute
 Cell Transformation, Neoplastic Antibodies  >4 publications with PSIP1 and Cell Transformation, Neoplastic
 Nervous System Diseases Antibodies  >4 publications with PSIP1 and Nervous System Diseases
 Brain Diseases Antibodies  >4 publications with PSIP1 and Brain Diseases
 Necrosis Antibodies  >4 publications with PSIP1 and Necrosis
 Adenocarcinoma Antibodies  >3 publications with PSIP1 and Adenocarcinoma
 Lung Diseases Antibodies  >2 publications with PSIP1 and Lung Diseases
 Breast Neoplasms Antibodies  >2 publications with PSIP1 and Breast Neoplasms
 Kidney Diseases Antibodies  >1 publications with PSIP1 and Kidney Diseases
 Hepatomegaly Antibodies  >1 publications with PSIP1 and Hepatomegaly

Organs/Tissues associated with PSIP1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >32 publications with PSIP1 and Blood
 Nerve Antibodies  >11 publications with PSIP1 and Nerve
 Connective Tissue Antibodies  >9 publications with PSIP1 and Connective Tissue
 Eye Antibodies  >8 publications with PSIP1 and Eye
 Brain Antibodies  >6 publications with PSIP1 and Brain
 Bone Antibodies  >5 publications with PSIP1 and Bone
 Prostate Antibodies  >4 publications with PSIP1 and Prostate
 Skin Antibodies  >4 publications with PSIP1 and Skin
 Bone Marrow Antibodies  >3 publications with PSIP1 and Bone Marrow
 Ganglia Antibodies  >3 publications with PSIP1 and Ganglia
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