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E3 Ubiquitin-Protein Ligase TRIM22 (TRIM22) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS763444

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human TRIM22 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human TRIM22 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
E3 ubiquitin-protein ligase TRIM22, ELISA Kit
Full Product Name
Human E3 ubiquitin-protein ligase TRIM22 ELISA Kit
Product Gene Name
TRIM22 elisa kit
Product Synonym Names
TRIM22/50 kDa-stimulated trans-acting factor/RING finger protein 94/Staf-50/Tripartite motif-containing protein 22/TRIM22/RNF94/STAF50/6.3.2.-
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
56,429 Da
OMIM
606559
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of TRIM22 elisa kit

Human TRIM22 ELISA Kit – Cat# MBS763444

Typical Standard Curve of TRIM22 elisa kit

Human TRIM22 ELISA Kit, Typical Standard Curve – Cat# MBS763444

Other Notes

Small volumes of TRIM22 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TRIM22 purchase

MBS763444 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the E3 ubiquitin-protein ligase TRIM22, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TRIM22. The ELISA analytical biochemical technique of the MBS763444 kit is based on TRIM22 antibody-TRIM22 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TRIM22 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TRIM22. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for TRIM22. It may not necessarily be applicable to this product.

NCBI GI #
313760629
NCBI GeneID
10346
NCBI Accession #
NP_001186502.1
UniProt Secondary Accession #
Q05CQ0; Q15521
UniProt Related Accession #
Q8IYM9
GenBank Nucleotide #
NM_001199573.1
NCBI Official Full Name
E3 ubiquitin-protein ligase TRIM22 isoform 2
NCBI Official Synonym Full Names
tripartite motif containing 22
NCBI Official Symbol
TRIM22
NCBI Official Synonym Symbols
RNF94; STAF50; GPSTAF50
UniProt Protein Name
E3 ubiquitin-protein ligase TRIM22
UniProt Synonym Protein Names
50 kDa-stimulated trans-acting factor; RING finger protein 94; Staf-50; Tripartite motif-containing protein 22
Protein Family
E3 ubiquitin-protein ligase
UniProt Gene Name
TRIM22
UniProt Synonym Gene Names
RNF94; STAF50
UniProt Entry Name
TRI22_HUMAN
NCBI Protein Information
E3 ubiquitin-protein ligase TRIM22

NCBI Summary for TRIM22

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the cytoplasm and its expression is induced by interferon. The protein down-regulates transcription from the HIV-1 LTR promoter region, suggesting that function of this protein may be to mediate interferon's antiviral effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
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UniProt Comments for TRIM22

TRIM22: Interferon-induced antiviral protein involved in cell innate immunity. The antiviral activity could in part be mediated by TRIM22-dependent ubiquitination of viral proteins. Plays a role in restricting the replication of HIV-1, encephalomyocarditis virus (EMCV) and hepatitis B virus (HBV). Acts as a transcriptional repressor of HBV core promoter. May have E3 ubiquitin-protein ligase activity. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; EC 6.3.2.-; Ubiquitin conjugating system; Ligase; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: Cajal body; cytoplasm; cytosol; nuclear speck; nucleoplasm; nucleus

Molecular Function: ligase activity; protein binding; protein binding, bridging; protein kinase binding; transcription corepressor activity; transcription factor activity; zinc ion binding

Biological Process: activation of NF-kappaB transcription factor; cytokine and chemokine mediated signaling pathway; defense response to virus; immune response; positive regulation of autophagy; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of transcription factor activity; protein ubiquitination; regulation of protein localization; regulation of transcription, DNA-dependent; response to virus; transcription, DNA-dependent; viral reproduction
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Research Articles on TRIM22

1. propose that TRIM22 is a direct target gene of PR and that it can mediate progesterone actions in uterine cells

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TRIM22 elisa kit

 Reference Product  PubMed Publications
 MX1 elisa kit  >5 publications with TRIM22 and MX1
 BST2 elisa kit  >5 publications with TRIM22 and BST2
 PML elisa kit  >2 publications with TRIM22 and PML
 ISG15 elisa kit  >1 publications with TRIM22 and ISG15
 EIF4E elisa kit  >1 publications with TRIM22 and EIF4E
 TAB2 elisa kit  >1 publications with TRIM22 and TAB2

Pathways associated with TRIM22 elisa kit

 Products by Pathway  Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Interferon Signaling Pathway antibodies  Interferon Signaling Pathway Diagram
 Interferon Gamma Signaling Pathway antibodies  Interferon Gamma Signaling Pathway Diagram

Diseases associated with TRIM22 elisa kit

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >4 publications with TRIM22 and Inflammation
 Liver Diseases Antibodies  >3 publications with TRIM22 and Liver Diseases
 Necrosis Antibodies  >3 publications with TRIM22 and Necrosis
 Influenza, Human Antibodies  >3 publications with TRIM22 and Influenza, Human
 Breast Neoplasms Antibodies  >2 publications with TRIM22 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >2 publications with TRIM22 and Cell Transformation, Neoplastic
 Kidney Diseases Antibodies  >2 publications with TRIM22 and Kidney Diseases
 Neoplasm Metastasis Antibodies  >1 publications with TRIM22 and Neoplasm Metastasis
 Carcinoma, Hepatocellular Antibodies  >1 publications with TRIM22 and Carcinoma, Hepatocellular

Organs/Tissues associated with TRIM22 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >17 publications with TRIM22 and Blood
 Liver Antibodies  >2 publications with TRIM22 and Liver
 Embryonic Tissue Antibodies  >2 publications with TRIM22 and Embryonic Tissue
 Uterus Antibodies  >2 publications with TRIM22 and Uterus
 Lung Antibodies  >2 publications with TRIM22 and Lung
 Prostate Antibodies  >1 publications with TRIM22 and Prostate
 Placenta Antibodies  >1 publications with TRIM22 and Placenta
 Kidney Antibodies  >1 publications with TRIM22 and Kidney
 Heart Antibodies  >1 publications with TRIM22 and Heart
 Bone Marrow Antibodies  >1 publications with TRIM22 and Bone Marrow
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