Amyloid Beta 42 (ABETA42) ELISA Kit for Mouse

Background: Amyloid beta 42(Abeta42) is a protein fragment that is derived from the protein precursor amyloid precursor protein (APP) and is the primary component of neuronal deposits. In Alzheimer's disease, Abeta42 aggregates into neurofibrillary tangles and neuritic plaques, which are one of the hallmark neuropathological features of the disease. Abeta42 is the longest fragment of Abeta and its formation is regulated by multiple enzymes. Under normal circumstances, Abeta42 exists in relatively low concentrations in the brain, but in Alzheimer's disease patients, its concentration increases significantly. In addition, the aggregation of Abeta42 can lead to neuronal death and inflammation, which is also one of the important causes of neuronal degeneration in Alzheimer's disease.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti Abeta42 antibody was precoated onto the 96-well plate. The biotin conjugated anti Abeta42 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with Abeta42 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of Abeta42 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

Small volumes of Abeta42 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS763460 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Amyloid Beta 42 (Abeta42) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Abeta42. The ELISA analytical biochemical technique of the MBS763460 kit is based on Abeta42 antibody-Abeta42 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Abeta42 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Abeta42. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).