Podocin (PDCN) ELISA Kit for Human

Name: Human PDCN ELISA Kit; Podocin; MyBioSource
Brand: MyBioSource
SKU: MBS763522
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS763522

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Podocin (PDCN), ELISA Kit

Full Product Name

Human Podocin ELISA Kit

Product Gene Name

PDCN elisa kit

Product Synonym Names

PDCN/NPHS2/Podocin

Species Reactivity

Human

Assay Type

Sandwich ELISA, Double Antibody

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

78.125-5000pg/ml

Sensitivity

46.875pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of PDCN. No significant cross-reactivity or interference between PDCN and analogues was observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

CV<10%

Intra-assay Precision

CV<8%

Molecular Weight

34,421 Da

OMIM

600995

3D Structure

ModBase 3D Structure for Q9NP85

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of PDCN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDCN purchase

MBS763522 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Podocin (PDCN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PDCN. The ELISA analytical biochemical technique of the MBS763522 kit is based on PDCN antibody-PDCN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDCN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDCN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PDCN. It may not necessarily be applicable to this product.

NCBI GI #

7363002

NCBI Accession #

CAB83216.1

UniProt Primary Accession #

Q9NP85

UniProt Secondary Accession #

Q8N6Q5; B1AM32; B1AM33

NCBI Official Full Name

podocin

UniProt Protein Name

Podocin

Protein Family

Podocin

UniProt Gene Name

NPHS2

UniProt Entry Name

PODO_HUMAN

UniProt Comments for PDCN

NPHS2: Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2). It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. Belongs to the band 7/mec-2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: endoplasmic reticulum; integral to plasma membrane; intercellular junction; lipid raft; plasma membrane; protein complex

Molecular Function: protein binding

Biological Process: actin cytoskeleton reorganization; excretion

Disease: Nephrotic Syndrome, Type 2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PDCN elisa kit

Reference Product	PubMed Publications
NPHS1 elisa kit	>85 publications with PDCN and NPHS1
CD2AP elisa kit	>65 publications with PDCN and CD2AP
TRPC6 elisa kit	>30 publications with PDCN and TRPC6
KIRREL elisa kit	>9 publications with PDCN and KIRREL
FYN elisa kit	>2 publications with PDCN and FYN
GRB2 elisa kit	>1 publications with PDCN and GRB2
KIRREL3 elisa kit	>1 publications with PDCN and KIRREL3

Diseases associated with PDCN elisa kit

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>401 publications with PDCN and Kidney Diseases
Nephrotic Syndrome Antibodies	>266 publications with PDCN and Nephrotic Syndrome
Proteinuria Antibodies	>251 publications with PDCN and Proteinuria
Nephrosis Antibodies	>230 publications with PDCN and Nephrosis
Glomerulosclerosis, Focal Segmental Antibodies	>152 publications with PDCN and Glomerulosclerosis, Focal Segmental
Disease Models, Animal Antibodies	>65 publications with PDCN and Disease Models, Animal
Albuminuria Antibodies	>54 publications with PDCN and Albuminuria
Renal Insufficiency Antibodies	>53 publications with PDCN and Renal Insufficiency
Nephrotic syndrome, idiopathic, steroid-resistant Antibodies	>30 publications with PDCN and Nephrotic syndrome, idiopathic, steroid-resistant
Hypertension Antibodies	>27 publications with PDCN and Hypertension

Organs/Tissues associated with PDCN elisa kit

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>410 publications with PDCN and Kidney