Peroxidasin Homolog (PXDN) ELISA Kit for Human

Name: Human PXDN ELISA Kit; Peroxidasin Homolog; MyBioSource
Brand: MyBioSource
SKU: MBS763931
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS763931

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Peroxidasin homolog (PXDN), ELISA Kit

Full Product Name

Human Peroxidasin homolog ELISA Kit

Product Gene Name

PXDN elisa kit

Product Synonym Names

PXDN/Peroxidasin homolog/p53-responsive gene 2 protein/Melanoma-associated antigen MG50/Vascular peroxidase 1/MG50/VPO/VPO1

Species Reactivity

Human

Assay Type

Sandwich ELISA, Double Antibody

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

78.125-5000pg/ml

Sensitivity

46.875pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of PXDN. No significant cross-reactivity or interference between PXDN and analogues was observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

CV<10%

Intra-assay Precision

CV<8%

Molecular Weight

80,329 Da

OMIM

269400

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of PXDN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PXDN purchase

MBS763931 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Peroxidasin homolog (PXDN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PXDN. The ELISA analytical biochemical technique of the MBS763931 kit is based on PXDN antibody-PXDN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PXDN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PXDN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PXDN. It may not necessarily be applicable to this product.

NCBI GI #

109150416

NCBI GeneID

7837

NCBI Accession #

NP_036425.1

UniProt Secondary Accession #

Q4KMG2; A8QM65; D6W4Y0

UniProt Related Accession #

Q92626

GenBank Nucleotide #

NM_012293.2

NCBI Official Full Name

peroxidasin homolog

NCBI Official Synonym Full Names

peroxidasin

NCBI Official Symbol

PXDN

NCBI Official Synonym Symbols

PXN; VPO; MG50; PRG2; COPOA; D2S448; D2S448E

UniProt Protein Name

Peroxidasin homolog

UniProt Synonym Protein Names

Melanoma-associated antigen MG50; Vascular peroxidase 1; p53-responsive gene 2 protein

Protein Family

Peroxidasin

UniProt Gene Name

PXDN

UniProt Synonym Gene Names

KIAA0230; MG50; PRG2; VPO; VPO1

UniProt Entry Name

PXDN_HUMAN

NCBI Protein Information

peroxidasin homolog

NCBI Summary for PXDN

This gene encodes a heme-containing peroxidase that is secreted into the extracellular matrix. It is involved in extracellular matrix formation, and may function in the physiological and pathological fibrogenic response in fibrotic kidney. Mutations in this gene cause corneal opacification and other ocular anomalies, and also microphthalmia and anterior segment dysgenesis. [provided by RefSeq, Aug 2014]

UniProt Comments for PXDN

PXDN: Displays low peroxidase activity and is likely to participate in H(2)O(2) metabolism and peroxidative reactions in the cardiovascular system. Plays a role in extracellular matrix formation. Belongs to the peroxidase family. XPO subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; EC 1.11.1.7; Secreted; Oxidoreductase

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: endoplasmic reticulum; extracellular matrix; extracellular space; proteinaceous extracellular matrix

Molecular Function: extracellular matrix structural constituent; heme binding; interleukin-1 receptor antagonist activity; metal ion binding; peroxidase activity

Biological Process: extracellular matrix organization and biogenesis; hydrogen peroxide catabolic process; immune response; negative regulation of cytokine and chemokine mediated signaling pathway; response to oxidative stress

Disease: Corneal Opacification With Other Ocular Anomalies

Research Articles on PXDN

1. The catalytic domain of peroxidasin and its immunoglobulin (Ig) domains are required for efficient sulfilimine bond formation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with PXDN elisa kit

Disease Name	Pubmed Publications
Congenital Abnormalities Antibodies	>2 publications with PXDN and Congenital Abnormalities
Inflammation Antibodies	>2 publications with PXDN and Inflammation
Leukemia, Myeloid, Acute Antibodies	>2 publications with PXDN and Leukemia, Myeloid, Acute
Neurologic Manifestations Antibodies	>1 publications with PXDN and Neurologic Manifestations
Carcinoma Antibodies	>1 publications with PXDN and Carcinoma

Organs/Tissues associated with PXDN elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>4 publications with PXDN and Brain
Eye Antibodies	>3 publications with PXDN and Eye
Heart Antibodies	>2 publications with PXDN and Heart
Vascular Antibodies	>2 publications with PXDN and Vascular
Prostate Antibodies	>1 publications with PXDN and Prostate
Bone Marrow Antibodies	>1 publications with PXDN and Bone Marrow
Liver Antibodies	>1 publications with PXDN and Liver
Kidney Antibodies	>1 publications with PXDN and Kidney
Uterus Antibodies	>1 publications with PXDN and Uterus
Skin Antibodies	>1 publications with PXDN and Skin