Macrophage Migration Inhibitory Factor (MIF) ELISA Kit for Human

Name: Human MIF ELISA Kit; Macrophage Migration Inhibitory Factor; MyBioSource
Brand: MyBioSource
SKU: MBS763962
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS763962

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MIF antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- MIF antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Macrophage migration inhibitory factor (MIF), ELISA Kit

Full Product Name

Human Macrophage migration inhibitory factor ELISA Kit

Product Gene Name

MIF elisa kit

Product Synonym Names

MIF (Macrophage migration inhibitory factor)/GIFmacrophage migration inhibitory factor/GLIF/Glycosylation-inhibiting factor/L-dopachrome isomerase/L-dopachrome tautomerase/macrophage migration inhibitory factor (glycosylation-inhibiting factor)/MMIF/Phenylpyruvate tautomerase

Species Reactivity

Human

Assay Type

Sandwich ELISA, Double Antibody

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

0.781-50ng/ml

Sensitivity

0.469ng/ml

Specificity

This assay has high sensitivity and excellent specificity for detection of MIF. No significant cross-reactivity or interference between MIF and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between MIF and all the analogues, therefore, cross reaction may still exist.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MIF were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MIF were tested 20 times on one plate, respectively. Intra-Assay: CV<8%

Molecular Weight

12,476 Da

OMIM

153620

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of MIF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MIF purchase

MBS763962 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Macrophage migration inhibitory factor (MIF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MIF. The ELISA analytical biochemical technique of the MBS763962 kit is based on MIF antibody-MIF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MIF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MIF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MIF. It may not necessarily be applicable to this product.

NCBI GI #

4505185

NCBI GeneID

4282

NCBI Accession #

NP_002406.1

UniProt Secondary Accession #

Q2V4Y5; Q6FHV0; A5Z1R8; B2R4S3

UniProt Related Accession #

P14174

GenBank Nucleotide #

NM_002415.1

NCBI Official Full Name

macrophage migration inhibitory factor

NCBI Official Synonym Full Names

macrophage migration inhibitory factor (glycosylation-inhibiting factor)

NCBI Official Symbol

MIF

NCBI Official Synonym Symbols

GIF; GLIF; MMIF

UniProt Protein Name

Macrophage migration inhibitory factor

UniProt Synonym Protein Names

Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase

Protein Family

Macrophage migration inhibitory factor

UniProt Gene Name

MIF

UniProt Synonym Gene Names

GLIF; MMIF; MIF; GIF

UniProt Entry Name

MIF_HUMAN

NCBI Protein Information

macrophage migration inhibitory factor

NCBI Summary for MIF

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]

UniProt Comments for MIF

MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.

Protein type: Amino Acid Metabolism - tyrosine; Isomerase; EC 5.3.2.1; Apoptosis; Cytokine; EC 5.3.3.12; Cell development/differentiation; Amino Acid Metabolism - phenylalanine

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: cell surface; cytoplasm; extracellular region; extracellular space; myelin sheath; nucleoplasm; vesicle

Molecular Function: chemoattractant activity; cytokine activity; dopachrome isomerase activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; phenylpyruvate tautomerase activity; protein binding; receptor binding

Biological Process: blood coagulation; carboxylic acid metabolic process; cell aging; cell proliferation; cell surface receptor linked signal transduction; DNA damage response, signal transduction by p53 class mediator; inflammatory response; innate immune response; leukocyte migration; negative regulation of apoptosis; negative regulation of cellular protein metabolic process; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of mature B cell apoptosis; negative regulation of myeloid cell apoptosis; positive chemotaxis; positive regulation of B cell proliferation; positive regulation of cytokine secretion; positive regulation of fibroblast proliferation; positive regulation of lipopolysaccharide-mediated signaling pathway; positive regulation of MAP kinase activity; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; prostaglandin biosynthetic process; regulation of macrophage activation

Disease: Rheumatoid Arthritis, Systemic Juvenile

Research Articles on MIF

1. Blockade of CXCR7 suppressed MIF-mediated ERK- and zeta-chain-associated protein kinase (ZAP)-70 activation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MIF elisa kit

Reference Product	PubMed Publications
CD74 elisa kit	>175 publications with MIF and CD74
MMP9 elisa kit	>17 publications with MIF and MMP9
COPS5 elisa kit	>17 publications with MIF and COPS5
TAT elisa kit	>3 publications with MIF and TAT
UBC elisa kit	>2 publications with MIF and UBC

Pathways associated with MIF elisa kit

Products by Pathway	Pathway Diagram
Adipogenesis Pathway antibodies	Adipogenesis Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
Phenylalanine Metabolism Pathway antibodies	Phenylalanine Metabolism Pathway Diagram
Phenylalanine Metabolism Pathway antibodies	Phenylalanine Metabolism Pathway Diagram
Spinal Cord Injury Pathway antibodies	Spinal Cord Injury Pathway Diagram
Tyrosine Metabolism Pathway antibodies	Tyrosine Metabolism Pathway Diagram
Tyrosine Metabolism Pathway antibodies	Tyrosine Metabolism Pathway Diagram

Diseases associated with MIF elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>762 publications with MIF and Inflammation
Necrosis Antibodies	>339 publications with MIF and Necrosis
Disease Models, Animal Antibodies	>254 publications with MIF and Disease Models, Animal
Carcinoma Antibodies	>175 publications with MIF and Carcinoma
Sepsis Antibodies	>145 publications with MIF and Sepsis
Arthritis, Rheumatoid Antibodies	>118 publications with MIF and Arthritis, Rheumatoid
Heart Diseases Antibodies	>117 publications with MIF and Heart Diseases
Pain Antibodies	>106 publications with MIF and Pain
Liver Diseases Antibodies	>90 publications with MIF and Liver Diseases
Pneumonia Antibodies	>89 publications with MIF and Pneumonia

Organs/Tissues associated with MIF elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1594 publications with MIF and Blood
Brain Antibodies	>422 publications with MIF and Brain
Vascular Antibodies	>275 publications with MIF and Vascular
Lung Antibodies	>230 publications with MIF and Lung
Skin Antibodies	>223 publications with MIF and Skin
Muscle Antibodies	>216 publications with MIF and Muscle
Liver Antibodies	>182 publications with MIF and Liver
Heart Antibodies	>174 publications with MIF and Heart
Spleen Antibodies	>168 publications with MIF and Spleen
Lymph Antibodies	>136 publications with MIF and Lymph