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Fatty Acid Synthase (FASN) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS763983

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human FASN antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human FASN antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Fatty acid synthase (FASN), ELISA Kit
Full Product Name
Human Fatty acid synthase ELISA Kit
Product Gene Name
FASN elisa kit
Product Synonym Names
FASN/Fatty acid synthase/FAS/OA-519/SDR27X1
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
273,427 Da
OMIM
600212
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of FASN elisa kit

Human FASN ELISA Kit – Cat# MBS763983

Typical Standard Curve of FASN elisa kit

Human FASN ELISA Kit, Typical Standard Curve – Cat# MBS763983

Other Notes

Small volumes of FASN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FASN purchase

MBS763983 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Fatty acid synthase (FASN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing FASN. The ELISA analytical biochemical technique of the MBS763983 kit is based on FASN antibody-FASN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect FASN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, FASN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for FASN. It may not necessarily be applicable to this product.

NCBI GI #
41872631
NCBI GeneID
2194
NCBI Accession #
NP_004095.4
UniProt Secondary Accession #
Q13479; Q16702; Q4LE83; Q6P4U5; Q6SS02; Q969R1; Q96C68; Q96IT0
UniProt Related Accession #
P49327
GenBank Nucleotide #
NM_004104.4
NCBI Official Full Name
fatty acid synthase
NCBI Official Synonym Full Names
fatty acid synthase
NCBI Official Symbol
FASN
NCBI Official Synonym Symbols
FAS; OA-519; SDR27X1
UniProt Protein Name
Fatty acid synthase
Protein Family
Fatty acid synthase
UniProt Gene Name
FASN
UniProt Synonym Gene Names
FAS
UniProt Entry Name
FAS_HUMAN
NCBI Protein Information
fatty acid synthase

NCBI Summary for FASN

The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq, Jul 2008]
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UniProt Comments for FASN

FASN: a multifunctional enzyme. Catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Protein type: EC 1.3.1.39; EC 2.3.1.39; EC 2.3.1.41; Lyase; Lipid Metabolism - fatty acid biosynthesis; EC 3.1.2.14; Hydrolase; Oxidoreductase; EC 1.1.1.100; EC 2.3.1.85; EC 2.3.1.38; Acetyltransferase; EC 4.2.1.59

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: cytoplasm; cytosol; glycogen granule; Golgi apparatus; melanosome; membrane; mitochondrion; plasma membrane

Molecular Function: 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase activity; 3-oxoacyl-[acyl-carrier-protein] reductase activity; 3-oxoacyl-[acyl-carrier-protein] synthase activity; [acyl-carrier-protein] S-acetyltransferase activity; [acyl-carrier-protein] S-malonyltransferase activity; drug binding; enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) activity; enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific) activity; fatty-acid synthase activity; myristoyl-[acyl-carrier-protein] hydrolase activity; oleoyl-[acyl-carrier-protein] hydrolase activity; palmitoyl-[acyl-carrier-protein] hydrolase activity; protein binding; protein homodimerization activity

Biological Process: acetyl-CoA metabolic process; cellular lipid metabolic process; energy reserve metabolic process; fatty acid biosynthetic process; fatty acid metabolic process; mammary gland development; osteoblast differentiation; pantothenate metabolic process; positive regulation of cellular metabolic process; triacylglycerol biosynthetic process; vitamin metabolic process; water-soluble vitamin metabolic process
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Research Articles on FASN

1. These results for the first time indicated that a cross talk in FASN and HER2 expressions might be associated with prognosis in malignant ovarian cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with FASN elisa kit

 Reference Product  PubMed Publications
 SREBF1 elisa kit  >127 publications with FASN and SREBF1
 ACACA elisa kit  >82 publications with FASN and ACACA
 LEP elisa kit  >10 publications with FASN and LEP
 ACACB elisa kit  >7 publications with FASN and ACACB
 INS elisa kit  >2 publications with FASN and INS

Pathways associated with FASN elisa kit

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of Gene Expression By SREBF (SREBP) Pathway antibodies  Activation Of Gene Expression By SREBF (SREBP) Pathway Diagram
 ChREBP Activates Metabolic Gene Expression Pathway antibodies  ChREBP Activates Metabolic Gene Expression Pathway Diagram
 Fatty Acid Biosynthesis Pathway antibodies  Fatty Acid Biosynthesis Pathway Diagram
 Fatty Acyl-CoA Biosynthesis Pathway antibodies  Fatty Acyl-CoA Biosynthesis Pathway Diagram
 Fatty Acid Biosynthesis Pathway antibodies  Fatty Acid Biosynthesis Pathway Diagram
 Fatty Acid Biosynthesis Pathway antibodies  Fatty Acid Biosynthesis Pathway Diagram
 Fatty Acid Biosynthesis, Elongation Pathway antibodies  Fatty Acid Biosynthesis, Elongation Pathway Diagram

Diseases associated with FASN elisa kit

 Disease Name  Pubmed Publications
 Fatty Liver Antibodies  >184 publications with FASN and Fatty Liver
 Obesity Antibodies  >136 publications with FASN and Obesity
 Liver Diseases Antibodies  >80 publications with FASN and Liver Diseases
 Insulin Resistance Antibodies  >65 publications with FASN and Insulin Resistance
 Breast Neoplasms Antibodies  >61 publications with FASN and Breast Neoplasms
 Inflammation Antibodies  >52 publications with FASN and Inflammation
 Disease Models, Animal Antibodies  >32 publications with FASN and Disease Models, Animal
 Weight Gain Antibodies  >28 publications with FASN and Weight Gain
 Weight Loss Antibodies  >26 publications with FASN and Weight Loss
 Carcinoma, Hepatocellular Antibodies  >26 publications with FASN and Carcinoma, Hepatocellular

Organs/Tissues associated with FASN elisa kit

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >215 publications with FASN and Liver
 Adipose Tissue Antibodies  >149 publications with FASN and Adipose Tissue
 Blood Antibodies  >134 publications with FASN and Blood
 Connective Tissue Antibodies  >93 publications with FASN and Connective Tissue
 Muscle Antibodies  >68 publications with FASN and Muscle
 Prostate Antibodies  >57 publications with FASN and Prostate
 Mammary Gland Antibodies  >43 publications with FASN and Mammary Gland
 Lung Antibodies  >27 publications with FASN and Lung
 Brain Antibodies  >27 publications with FASN and Brain
 Kidney Antibodies  >19 publications with FASN and Kidney
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