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Protein Wnt-2b (WNT2B) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS764038

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Protein Wnt-2b (WNT2B), ELISA Kit
Full Product Name
Human Protein Wnt-2b ELISA Kit
Product Gene Name
WNT2B elisa kit
Product Synonym Names
WNT2B/WNT13/Protein Wnt-13/XWNT2/protein Wnt-2b/wingless-type MMTV integration site family; member 2B/XWNT2; Xenopus; homolog of/XWNT2wingless-type MMTV integration site family; member 13
Species Reactivity
Human
Assay Type
Sandwich
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
15.625-1000pg/ml
Sensitivity
9.375pg/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of WNT2B. No significant cross-reactivity or interference between WNT2B and analogues was observed.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Inter-assay Precision
CV<10%
Intra-assay Precision
CV<8%
Molecular Weight
41,830 Da
OMIM
601968
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of WNT2B elisa kit

Human WNT2B ELISA Kit – Cat# MBS764038

Other Notes

Small volumes of WNT2B elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for WNT2B purchase

MBS764038 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein Wnt-2b (WNT2B) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT2B. The ELISA analytical biochemical technique of the MBS764038 kit is based on WNT2B antibody-WNT2B antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT2B antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT2B. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for WNT2B. It may not necessarily be applicable to this product.

NCBI GI #
630044901
NCBI GeneID
7482
NCBI Accession #
NP_001278809.1
UniProt Secondary Accession #
O14903; Q5TEH9; Q5TEI2; Q9HDC1; Q9HDC2
UniProt Related Accession #
Q93097
GenBank Nucleotide #
NM_001291880.1
NCBI Official Full Name
protein Wnt-2b isoform 3
NCBI Official Synonym Full Names
wingless-type MMTV integration site family member 2B
NCBI Official Symbol
WNT2B
NCBI Official Synonym Symbols
WNT13
UniProt Protein Name
Protein Wnt-2b
UniProt Synonym Protein Names
Protein Wnt-13
Protein Family
Protein
UniProt Gene Name
WNT2B
UniProt Synonym Gene Names
WNT13
UniProt Entry Name
WNT2B_HUMAN
NCBI Protein Information
protein Wnt-2b

NCBI Summary for WNT2B

This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as carcinogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
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UniProt Comments for WNT2B

WNT2B: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. May be involved in normal development or differentiation as well as in carcinogenesis. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: frizzled binding

Biological Process: cell fate commitment; cellular response to starvation; chondrocyte differentiation; forebrain regionalization; lens development in camera-type eye; male gonad development; neuron differentiation; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin
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Research Articles on WNT2B

1. ACSL5 mediates antiproliferative activities via Wnt2B palmitoylation with diminished Wnt activity. The molecular pathway is probably relevant for intestinal homeostasis, overwhelmed by other pathways in carcinogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with WNT2B elisa kit

 Reference Product  PubMed Publications
 FZD5 elisa kit  >10 publications with WNT2B and FZD5
 FZD4 elisa kit  >10 publications with WNT2B and FZD4
 FZD6 elisa kit  >10 publications with WNT2B and FZD6
 FZD2 elisa kit  >9 publications with WNT2B and FZD2
 FZD10 elisa kit  >8 publications with WNT2B and FZD10
 LRP6 elisa kit  >6 publications with WNT2B and LRP6
 FZD3 elisa kit  >6 publications with WNT2B and FZD3
 FZD8 elisa kit  >5 publications with WNT2B and FZD8

Pathways associated with WNT2B elisa kit

 Products by Pathway  Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Class B/2 (Secretin Family Receptors) Pathway antibodies  Class B/2 (Secretin Family Receptors) Pathway Diagram
 DNA Damage Response (only ATM Dependent) Pathway antibodies  DNA Damage Response (only ATM Dependent) Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram

Diseases associated with WNT2B elisa kit

 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >8 publications with WNT2B and Breast Neoplasms
 Lung Neoplasms Antibodies  >6 publications with WNT2B and Lung Neoplasms
 Inflammation Antibodies  >5 publications with WNT2B and Inflammation
 Disease Models, Animal Antibodies  >4 publications with WNT2B and Disease Models, Animal
 Necrosis Antibodies  >4 publications with WNT2B and Necrosis
 Neoplasm Metastasis Antibodies  >3 publications with WNT2B and Neoplasm Metastasis
 Neural Tube Defects Antibodies  >3 publications with WNT2B and Neural Tube Defects
 Ovarian Neoplasms Antibodies  >2 publications with WNT2B and Ovarian Neoplasms
 Hyperplasia Antibodies  >2 publications with WNT2B and Hyperplasia
 Genital Diseases, Female Antibodies  >2 publications with WNT2B and Genital Diseases, Female

Organs/Tissues associated with WNT2B elisa kit

 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >28 publications with WNT2B and Embryonic Tissue
 Eye Antibodies  >22 publications with WNT2B and Eye
 Brain Antibodies  >19 publications with WNT2B and Brain
 Lung Antibodies  >18 publications with WNT2B and Lung
 Kidney Antibodies  >8 publications with WNT2B and Kidney
 Testis Antibodies  >6 publications with WNT2B and Testis
 Skin Antibodies  >4 publications with WNT2B and Skin
 Ovary Antibodies  >4 publications with WNT2B and Ovary
 Prostate Antibodies  >4 publications with WNT2B and Prostate
 Placenta Antibodies  >4 publications with WNT2B and Placenta
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