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Creatine Kinase M-Type (CKM) ELISA Kit for Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS764672

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Ckm antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Ckm antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Creatine kinase M-type (Ckm), ELISA Kit
Full Product Name
Mouse Creatine kinase M-type ELISA Kit
Product Gene Name
Ckm elisa kit
Product Synonym Names
Ckm/CKMM/M-CK/Creatine kinase M chain/creatine kinase; muscle/creatine kinase-M
Species Reactivity
Mouse
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
1.563-100ng/ml
Sensitivity
0.938ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
43,101 Da
OMIM
123310
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of Ckm elisa kit

Mouse CKM ELISA Kit – Cat# MBS764672

Typical Standard Curve of Ckm elisa kit

Mouse CKM ELISA Kit, Typical Standard Curve – Cat# MBS764672

Other Notes

Small volumes of Ckm elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ckm purchase

MBS764672 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Creatine kinase M-type (Ckm) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Ckm. The ELISA analytical biochemical technique of the MBS764672 kit is based on Ckm antibody-Ckm antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Ckm antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Ckm. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Ckm. It may not necessarily be applicable to this product.

NCBI GI #
21536288
NCBI GeneID
1158
NCBI Accession #
NP_001815.2
UniProt Secondary Accession #
Q96QL9
UniProt Related Accession #
P06732
GenBank Nucleotide #
NM_001824.4
NCBI Official Full Name
creatine kinase M-type
NCBI Official Synonym Full Names
creatine kinase, M-type
NCBI Official Symbol
CKM
NCBI Official Synonym Symbols
CKMM; M-CK
UniProt Protein Name
Creatine kinase M-type
UniProt Synonym Protein Names
Creatine kinase M chain; M-CK
Protein Family
Creatine kinase
UniProt Gene Name
CKM
UniProt Synonym Gene Names
CKMM
UniProt Entry Name
KCRM_HUMAN
NCBI Protein Information
creatine kinase M-type

NCBI Summary for Ckm

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]
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UniProt Comments for Ckm

CKM: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; EC 2.7.3.2; Kinase, other

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytosol

Molecular Function: ATP binding; creatine kinase activity; protein binding

Biological Process: creatine metabolic process; phosphocreatine biosynthetic process; phosphorylation; polyamine metabolic process
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Research Articles on Ckm

1. CK-MB mass is more significant criteria of myocardial injury.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Ckm elisa kit

 Reference Product  PubMed Publications
 CKB elisa kit  >15 publications with Ckm and CKB
 GAMT elisa kit  >1 publications with Ckm and GAMT

Pathways associated with Ckm elisa kit

 Products by Pathway  Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Creatine Metabolism Pathway antibodies  Creatine Metabolism Pathway Diagram
 Creatine Pathway antibodies  Creatine Pathway Diagram
 Creatine Pathway antibodies  Creatine Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Metabolism Of Polyamines Pathway antibodies  Metabolism Of Polyamines Pathway Diagram
 Regulation Of Retinoblastoma Protein Pathway antibodies  Regulation Of Retinoblastoma Protein Pathway Diagram

Diseases associated with Ckm elisa kit

 Disease Name  Pubmed Publications
 Heart Diseases Antibodies  >13 publications with Ckm and Heart Diseases
 Kidney Diseases Antibodies  >7 publications with Ckm and Kidney Diseases
 Myocardial Infarction Antibodies  >7 publications with Ckm and Myocardial Infarction
 Disease Models, Animal Antibodies  >6 publications with Ckm and Disease Models, Animal
 Inflammation Antibodies  >4 publications with Ckm and Inflammation
 Lung Neoplasms Antibodies  >4 publications with Ckm and Lung Neoplasms
 Liver Neoplasms Antibodies  >4 publications with Ckm and Liver Neoplasms
 Ischemia Antibodies  >4 publications with Ckm and Ischemia
 Weight Loss Antibodies  >3 publications with Ckm and Weight Loss
 Necrosis Antibodies  >3 publications with Ckm and Necrosis

Organs/Tissues associated with Ckm elisa kit

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >64 publications with Ckm and Muscle
 Heart Antibodies  >28 publications with Ckm and Heart
 Kidney Antibodies  >12 publications with Ckm and Kidney
 Brain Antibodies  >10 publications with Ckm and Brain
 Liver Antibodies  >8 publications with Ckm and Liver
 Lung Antibodies  >6 publications with Ckm and Lung
 Embryonic Tissue Antibodies  >4 publications with Ckm and Embryonic Tissue
 Bone Antibodies  >4 publications with Ckm and Bone
 Vascular Antibodies  >4 publications with Ckm and Vascular
 Nerve Antibodies  >2 publications with Ckm and Nerve
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