Claudin-3 (CLDN3) ELISA Kit for Mouse

Background: Claudin-3 is the product of an intronless gene (CLDN3) whose expression is markedly dysregulated in distinct cancer subtypes, including ovarian and breast carcinomas. Reduced claudin-3 expression is also a hallmark of a subset of breast tumors known as the claudin-low subtype, in which reduced claudin-3 expression was shown to correlate with a phenotype characteristic of cells undergoing epithelial-mesenchymal transition.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti Cldn3 antibody was precoated onto the 96-well plate. The biotin conjugated anti Cldn3 antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with Cldn3 conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of Cldn3 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

Small volumes of Cldn3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS764676 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Claudin-3 (Cldn3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Cldn3. The ELISA analytical biochemical technique of the MBS764676 kit is based on Cldn3 antibody-Cldn3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Cldn3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Cldn3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).