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Protein Disulfide Isomerase A3 (PDIA3) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS764749

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PDIA3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PDIA3 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Protein Disulfide Isomerase A3 (PDIA3), ELISA Kit
Full Product Name
Human Protein Disulfide Isomerase A3 ELISA Kit
Product Gene Name
PDIA3 elisa kit
Product Synonym Names
PDIA3/ERp60/GRP58/PDIA3/Disulfide isomerase ER-60/EC 5.3.4.1/endoplasmic reticulum P58/Endoplasmic reticulum resident protein 57/Endoplasmic reticulum resident protein 60/ER protein 60/ER60/ERP57/ERp5758 kDa glucose-regulated protein/ERP60/ERp6058 kDa microsomal protein/ERp61/glucose regulated protein; 58kDa/GRP57/GRP58ER protein 57/HsT17083/P58/p58/phospholipase C-alpha/PI-PLC/protein disulfide isomerase family A; member 3/protein disulfide isomerase-associated 3/protein disulfide-isomerase A3
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
56,782 Da
OMIM
602046
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of PDIA3 elisa kit

Human PDIA3 ELISA Kit – Cat# MBS764749

Typical Standard Curve of PDIA3 elisa kit

Human PDIA3 ELISA Kit, Typical Standard Curve – Cat# MBS764749

Other Notes

Small volumes of PDIA3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDIA3 purchase

MBS764749 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein Disulfide Isomerase A3 (PDIA3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PDIA3. The ELISA analytical biochemical technique of the MBS764749 kit is based on PDIA3 antibody-PDIA3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PDIA3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PDIA3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PDIA3. It may not necessarily be applicable to this product.

NCBI GI #
21361657
NCBI GeneID
2923
NCBI Accession #
NP_005304.3
UniProt Secondary Accession #
Q13453; Q14255; Q8IYF8; Q9UMU7
UniProt Related Accession #
P30101; AAB37397
GenBank Nucleotide #
NM_005313.4
NCBI Official Full Name
protein disulfide-isomerase A3
NCBI Official Synonym Full Names
protein disulfide isomerase family A member 3
NCBI Official Symbol
PDIA3
NCBI Official Synonym Symbols
P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
UniProt Protein Name
Protein disulfide-isomerase A3
UniProt Synonym Protein Names
58 kDa glucose-regulated protein; 58 kDa microsomal protein; p58; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; ER protein 57; ERp57; Endoplasmic reticulum resident protein 60; ER protein 60; ERp60
Protein Family
Protein disulfide-isomerase
UniProt Gene Name
PDIA3
UniProt Synonym Gene Names
ERP57; ERP60; GRP58; p58; ER protein 57; ERp57; ER protein 60; ERp60
UniProt Entry Name
PDIA3_HUMAN
NCBI Protein Information
protein disulfide-isomerase A3

NCBI Summary for PDIA3

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]
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UniProt Comments for PDIA3

GRP58: a protein disulfide isomerase found in the endoplasmic reticulum lumen. Catalyzes the rearrangement of both intrachain and interchain disulfide bonds in proteins to form the native structures. An essential component of the peptide-loading complex of the major histocompatibility complex class I pathway.

Protein type: Isomerase; Chaperone; Protease; EC 5.3.4.1; Endoplasmic reticulum; Phospholipase; Apoptosis

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: cell surface; endoplasmic reticulum; endoplasmic reticulum lumen; focal adhesion; melanosome; myelin sheath; nucleus

Molecular Function: cysteine-type endopeptidase activity; disulfide oxidoreductase activity; phospholipase C activity; protein binding; protein disulfide isomerase activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; cell redox homeostasis; cellular protein metabolic process; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; protein folding; protein import into nucleus; protein retention in ER; proteolysis; signal transduction
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Research Articles on PDIA3

1. study identifies ERp57 as a new modulator of PrP levels and may help with understanding the consequences of ERp57 up-regulation observed in human disease

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PDIA3 elisa kit

 Reference Product  PubMed Publications
 HLA-A elisa kit  >5 publications with PDIA3 and HLA-A
 CALR elisa kit  >5 publications with PDIA3 and CALR
 HSPA5 elisa kit  >3 publications with PDIA3 and HSPA5
 ERAP1 elisa kit  >2 publications with PDIA3 and ERAP1
 CANX elisa kit  >2 publications with PDIA3 and CANX
 ERO1L elisa kit  >1 publications with PDIA3 and ERO1L
 PPIB elisa kit  >1 publications with PDIA3 and PPIB
 ERP27 elisa kit  >1 publications with PDIA3 and ERP27

Pathways associated with PDIA3 elisa kit

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway antibodies  Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing-Cross Presentation Pathway antibodies  Antigen Processing-Cross Presentation Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Calnexin/calreticulin Cycle Pathway antibodies  Calnexin/calreticulin Cycle Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 ER-Phagosome Pathway antibodies  ER-Phagosome Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram

Diseases associated with PDIA3 elisa kit

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >17 publications with PDIA3 and Disease Models, Animal
 Inflammation Antibodies  >15 publications with PDIA3 and Inflammation
 Nervous System Diseases Antibodies  >15 publications with PDIA3 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >12 publications with PDIA3 and Cardiovascular Diseases
 Brain Diseases Antibodies  >11 publications with PDIA3 and Brain Diseases
 Liver Diseases Antibodies  >10 publications with PDIA3 and Liver Diseases
 Liver Neoplasms Antibodies  >7 publications with PDIA3 and Liver Neoplasms
 Immune System Diseases Antibodies  >7 publications with PDIA3 and Immune System Diseases
 Necrosis Antibodies  >6 publications with PDIA3 and Necrosis
 Fibrosis Antibodies  >5 publications with PDIA3 and Fibrosis

Organs/Tissues associated with PDIA3 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >39 publications with PDIA3 and Blood
 Liver Antibodies  >23 publications with PDIA3 and Liver
 Brain Antibodies  >17 publications with PDIA3 and Brain
 Bone Antibodies  >16 publications with PDIA3 and Bone
 Intestine Antibodies  >14 publications with PDIA3 and Intestine
 Kidney Antibodies  >14 publications with PDIA3 and Kidney
 Lung Antibodies  >12 publications with PDIA3 and Lung
 Muscle Antibodies  >12 publications with PDIA3 and Muscle
 Connective Tissue Antibodies  >10 publications with PDIA3 and Connective Tissue
 Vascular Antibodies  >9 publications with PDIA3 and Vascular
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