Cartilage Acidic Protein 1 (CRTAC1) ELISA Kit for Mouse

Name: Mouse CRTAC1 ELISA Kit; Cartilage Acidic Protein 1; MyBioSource
Brand: MyBioSource
SKU: MBS764804
Price: 405 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS764804

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CRP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CRP antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Cartilage acidic protein 1 (Crtac1), ELISA Kit

Full Product Name

Mouse Cartilage acidic protein 1 ELISA Kit

Product Gene Name

Crtac1 elisa kit

Product Synonym Names

Crtac1/Aspic1/Cep68/Protein CRTAC1-B/68 kDa chondrocyte-expressed protein/CEP-68/ASPIC

Species Reactivity

Mouse

Assay Type

Sandwich ELISA, Double Antibody

Samples

Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples

Detection Range

78.125-5000pg/ml

Sensitivity

46.875pg/ml

Specificity

This assay has high sensitivity and excellent specificity for detection ofCRP. No significant crossreactivity or interference betweenCRP and analogues was observed.

Preparation and Storage

Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high levelCRP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high levelCRP were tested 20 times on one plate, respectively. Intra-Assay: CV<8%

Molecular Weight

68,059 Da

OMIM

606276

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of Crtac1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Crtac1 purchase

MBS764804 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Cartilage acidic protein 1 (Crtac1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Crtac1. The ELISA analytical biochemical technique of the MBS764804 kit is based on Crtac1 antibody-Crtac1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Crtac1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Crtac1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Crtac1. It may not necessarily be applicable to this product.

NCBI GI #

47777317

NCBI GeneID

55118

NCBI Accession #

NP_060528.3

UniProt Secondary Accession #

Q5T4F8; Q8N4H6; Q8TE52; Q9NQ78; Q9NQ80; Q9NW46; B1ALN4

UniProt Related Accession #

Q9NQ79

GenBank Nucleotide #

NM_018058.6

NCBI Official Full Name

cartilage acidic protein 1 isoform A

NCBI Official Synonym Full Names

cartilage acidic protein 1

NCBI Official Symbol

CRTAC1

NCBI Official Synonym Symbols

ASPIC; ASPIC1; CEP-68

UniProt Protein Name

Cartilage acidic protein 1

UniProt Synonym Protein Names

68 kDa chondrocyte-expressed protein; CEP-68; ASPIC

Protein Family

Cartilage acidic protein

UniProt Gene Name

CRTAC1

UniProt Synonym Gene Names

ASPIC1; CEP68; CEP-68

UniProt Entry Name

CRAC1_HUMAN

NCBI Protein Information

cartilage acidic protein 1

NCBI Summary for Crtac1

This gene encodes a glycosylated extracellular matrix protein that is found in the interterritorial matrix of articular deep zone cartilage. This protein is used as a marker to distinguish chondrocytes from osteoblasts and mesenchymal stem cells in culture. The presence of FG-GAP motifs and an RGD integrin-binding motif suggests that this protein may be involved in cell-cell or cell-matrix interactions. Copy number alterations in this gene have been observed in neurofibromatosis type 1-associated glomus tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

UniProt Comments for Crtac1

CRTAC1: a glycosylated extracellular matrix protein that is found in the interterritorial matrix of articular deep zone cartilage. This protein is used as a marker to distinguish chondrocytes from osteoblasts and mesenchymal stem cells in culture. The presence of FG-GAP motifs and an RGD integrin-binding motif suggests that this protein may be involved in cell-cell or cell-matrix interactions. Copy number alterations in this gene have been observed in neurofibromatosis type 1-associated glomus tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: growth cone; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; protein binding

Biological Process: axonal fasciculation; olfactory bulb development

Research Articles on Crtac1

1. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with Crtac1 elisa kit

Disease Name	Pubmed Publications
Abnormalities, Multiple Antibodies	>1 publications with Crtac1 and Abnormalities, Multiple
Inflammation Antibodies	>1 publications with Crtac1 and Inflammation

Organs/Tissues associated with Crtac1 elisa kit

Organ/Tissue Name	Pubmed Publications
Bone Antibodies	>5 publications with Crtac1 and Bone
Brain Antibodies	>4 publications with Crtac1 and Brain
Lung Antibodies	>3 publications with Crtac1 and Lung
Connective Tissue Antibodies	>2 publications with Crtac1 and Connective Tissue
Skin Antibodies	>1 publications with Crtac1 and Skin
Eye Antibodies	>1 publications with Crtac1 and Eye