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Creatine Kinase B-Type (CKB) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS764850

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human CKB antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CKB antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Creatine kinase B-type (CKB), ELISA Kit
Full Product Name
Human Creatine kinase B-type ELISA Kit
Product Gene Name
CKB elisa kit
Product Synonym Names
CKB (Creatine kinase B-type)/B-CK/Creatine kinase B chain
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
42,644 Da
OMIM
123280
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of CKB elisa kit

Human CKB ELISA Kit – Cat# MBS764850

Typical Standard Curve of CKB elisa kit

Human CKB ELISA Kit, Typical Standard Curve – Cat# MBS764850

Other Notes

Small volumes of CKB elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CKB purchase

MBS764850 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Creatine kinase B-type (CKB) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing CKB. The ELISA analytical biochemical technique of the MBS764850 kit is based on CKB antibody-CKB antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect CKB antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, CKB. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for CKB. It may not necessarily be applicable to this product.

NCBI GI #
21536286
NCBI GeneID
1152
NCBI Accession #
NP_001814.2
UniProt Secondary Accession #
Q2LE07; Q6FG40; Q9UC66; A8K236; B2R5R4
UniProt Related Accession #
P12277
GenBank Nucleotide #
NM_001823.4
NCBI Official Full Name
creatine kinase B-type
NCBI Official Synonym Full Names
creatine kinase, brain
NCBI Official Symbol
CKB
NCBI Official Synonym Symbols
BCK; B-CK; CKBB; HEL-211; HEL-S-29
UniProt Protein Name
Creatine kinase B-type
UniProt Synonym Protein Names
B-CK; Creatine kinase B chain
Protein Family
Creatine kinase
UniProt Gene Name
CKB
UniProt Synonym Gene Names
CKBB
UniProt Entry Name
KCRB_HUMAN
NCBI Protein Information
creatine kinase B-type

NCBI Summary for CKB

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for CKB

CKB: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; EC 2.7.3.2; Kinase, other

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: cytoplasm; cytosol; extracellular space; mitochondrion; myelin sheath

Molecular Function: ATP binding; creatine kinase activity; protein binding; ubiquitin protein ligase binding

Biological Process: cellular chloride ion homeostasis; creatine metabolic process; phosphorylation; polyamine metabolic process; substantia nigra development
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Research Articles on CKB

1. CK-MB levels were higher after ERCP in non-ischemic patients compared with a myocardial ischemia group. Creatine phosphokinase levels did not differ significantly between groups.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with CKB elisa kit

 Reference Product  PubMed Publications
 CKM elisa kit  >15 publications with CKB and CKM
 ASB9 elisa kit  >2 publications with CKB and ASB9
 LDHA elisa kit  >1 publications with CKB and LDHA
 LDHB elisa kit  >1 publications with CKB and LDHB
 GAMT elisa kit  >1 publications with CKB and GAMT

Pathways associated with CKB elisa kit

 Products by Pathway  Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Creatine Metabolism Pathway antibodies  Creatine Metabolism Pathway Diagram
 Creatine Pathway antibodies  Creatine Pathway Diagram
 Creatine Pathway antibodies  Creatine Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Metabolism Of Polyamines Pathway antibodies  Metabolism Of Polyamines Pathway Diagram
 Urea Cycle And Metabolism Of Amino Groups Pathway antibodies  Urea Cycle And Metabolism Of Amino Groups Pathway Diagram

Diseases associated with CKB elisa kit

 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >19 publications with CKB and Brain Diseases
 Nervous System Diseases Antibodies  >14 publications with CKB and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >10 publications with CKB and Cardiovascular Diseases
 Heart Diseases Antibodies  >8 publications with CKB and Heart Diseases
 Disease Models, Animal Antibodies  >6 publications with CKB and Disease Models, Animal
 Movement Disorders Antibodies  >5 publications with CKB and Movement Disorders
 Liver Diseases Antibodies  >5 publications with CKB and Liver Diseases
 Adenocarcinoma Antibodies  >5 publications with CKB and Adenocarcinoma
 Cognition Disorders Antibodies  >4 publications with CKB and Cognition Disorders
 Neoplasms, Experimental Antibodies  >4 publications with CKB and Neoplasms, Experimental

Organs/Tissues associated with CKB elisa kit

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >64 publications with CKB and Brain
 Blood Antibodies  >32 publications with CKB and Blood
 Muscle Antibodies  >18 publications with CKB and Muscle
 Heart Antibodies  >14 publications with CKB and Heart
 Uterus Antibodies  >13 publications with CKB and Uterus
 Liver Antibodies  >8 publications with CKB and Liver
 Nerve Antibodies  >8 publications with CKB and Nerve
 Lung Antibodies  >7 publications with CKB and Lung
 Bone Antibodies  >6 publications with CKB and Bone
 Embryonic Tissue Antibodies  >4 publications with CKB and Embryonic Tissue
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