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Dipeptidase 1 (DPEP1) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS765214

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DPEP1. During the reaction,Human DPEP1 in the sample or standard competes with a… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Dipeptidase 1 (DPEP1), ELISA Kit
Full Product Name
Human Dipeptidase 1 ELISA Kit
Product Gene Name
DPEP1 elisa kit
Product Synonym Names
DPEP1/Renal dipeptidase/hRDP/Dehydropeptidase-I/Microsomal dipeptidase/MDP/RDP
Species Reactivity
Human
Assay Type
Competitive ELISA, Coated with Antigen
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
45,674 Da
OMIM
179780
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of DPEP1 elisa kit

Human DPEP1 ELISA Kit – Cat# MBS765214

Typical Standard Curve of DPEP1 elisa kit

Human DPEP1 ELISA Kit, Typical Standard Curve – Cat# MBS765214

Other Notes

Small volumes of DPEP1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DPEP1 purchase

MBS765214 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Dipeptidase 1 (DPEP1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DPEP1. The ELISA analytical biochemical technique of the MBS765214 kit is based on DPEP1 antibody-DPEP1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DPEP1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DPEP1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DPEP1. It may not necessarily be applicable to this product.

NCBI GI #
189458885
NCBI GeneID
1800
NCBI Accession #
NP_001121613.1
UniProt Secondary Accession #
Q96AK2; D3DX80
UniProt Related Accession #
P16444
GenBank Nucleotide #
NM_001128141.2
NCBI Official Full Name
dipeptidase 1
NCBI Official Synonym Full Names
dipeptidase 1 (renal)
NCBI Official Symbol
DPEP1
NCBI Official Synonym Symbols
MDP; RDP; MBD1
UniProt Protein Name
Dipeptidase 1
UniProt Synonym Protein Names
Dehydropeptidase-I; Microsomal dipeptidase; Renal dipeptidase; hRDP
Protein Family
Dipeptidase
UniProt Gene Name
DPEP1
UniProt Synonym Gene Names
MDP; RDP; hRDP
UniProt Entry Name
DPEP1_HUMAN
NCBI Protein Information
dipeptidase 1

NCBI Summary for DPEP1

The protein encoded by this gene is a kidney membrane enzyme involved in the metabolism of glutathione and other similar proteins by dipeptide hydrolysis. The encoded protein is known to regulate leukotriene activity by catalyzing the conversion of leukotriene D4 to leukotriene E4. This protein uses zinc as a cofactor and acts as a disulfide-linked homodimer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2012]
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UniProt Comments for DPEP1

DPEP1: Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity. Belongs to the peptidase M19 family.

Protein type: EC 3.4.13.19; Membrane protein, GPI anchor; Protease

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: apical part of cell; apical plasma membrane; extracellular space; microvillus membrane; plasma membrane

Molecular Function: caspase inhibitor activity; dipeptidyl-peptidase activity; metalloexopeptidase activity; protein binding; zinc ion binding

Biological Process: antibiotic metabolic process; arachidonic acid metabolic process; glutathione metabolic process; homocysteine metabolic process; leukotriene metabolic process; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of cell migration; proteolysis; xenobiotic metabolic process
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Research Articles on DPEP1

1. Dipeptidase 1 has been identified as an excellent marker of high-grade IEN and CRC, and may thus be applied for screening of early neoplastic lesions and for prognostic stratification.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with DPEP1 elisa kit

 Products by Pathway  Pathway Diagram
 Aflatoxin Activation And Detoxification Pathway antibodies  Aflatoxin Activation And Detoxification Pathway Diagram
 Arachidonic Acid Metabolism Pathway antibodies  Arachidonic Acid Metabolism Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Eicosanoid Synthesis Pathway antibodies  Eicosanoid Synthesis Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Synthesis Of Leukotrienes (LT) And Eoxins (EX) Pathway antibodies  Synthesis Of Leukotrienes (LT) And Eoxins (EX) Pathway Diagram
 Leukotriene Biosynthesis Pathway antibodies  Leukotriene Biosynthesis Pathway Diagram
 Leukotriene Biosynthesis Pathway antibodies  Leukotriene Biosynthesis Pathway Diagram

Diseases associated with DPEP1 elisa kit

 Disease Name  Pubmed Publications
 Cell Transformation, Neoplastic Antibodies  >2 publications with DPEP1 and Cell Transformation, Neoplastic
 Congenital Abnormalities Antibodies  >1 publications with DPEP1 and Congenital Abnormalities
 Ovarian Diseases Antibodies  >1 publications with DPEP1 and Ovarian Diseases
 Nervous System Diseases Antibodies  >1 publications with DPEP1 and Nervous System Diseases
 Ovarian Neoplasms Antibodies  >1 publications with DPEP1 and Ovarian Neoplasms
 Proteinuria Antibodies  >1 publications with DPEP1 and Proteinuria
 Neoplasms, Experimental Antibodies  >1 publications with DPEP1 and Neoplasms, Experimental
 Breast Neoplasms Antibodies  >1 publications with DPEP1 and Breast Neoplasms
 Kidney Diseases Antibodies  >1 publications with DPEP1 and Kidney Diseases

Organs/Tissues associated with DPEP1 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >5 publications with DPEP1 and Kidney
 Intestine Antibodies  >4 publications with DPEP1 and Intestine
 Lymph Node Antibodies  >2 publications with DPEP1 and Lymph Node
 Pancreas Antibodies  >1 publications with DPEP1 and Pancreas
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