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Iron-Responsive Element-Binding Protein 2 (IREB2) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS765327

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human IREB2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human IREB2 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Iron-responsive element-binding protein 2 (IREB2), ELISA Kit
Full Product Name
Human Iron-responsive element-binding protein 2 ELISA Kit
Product Gene Name
IREB2 elisa kit
Product Synonym Names
IREB2/IRE-BP 2/IRP2/Iron regulatory protein 2
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
37,487 Da
OMIM
147582
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of IREB2 elisa kit

Human IREB2 ELISA Kit – Cat# MBS765327

Typical Standard Curve of IREB2 elisa kit

Human IREB2 ELISA Kit, Typical Standard Curve – Cat# MBS765327

Other Notes

Small volumes of IREB2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IREB2 purchase

MBS765327 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Iron-responsive element-binding protein 2 (IREB2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IREB2. The ELISA analytical biochemical technique of the MBS765327 kit is based on IREB2 antibody-IREB2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IREB2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IREB2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for IREB2. It may not necessarily be applicable to this product.

NCBI GI #
41352693
NCBI GeneID
3658
NCBI Accession #
NP_004127.1
UniProt Secondary Accession #
Q13095; Q1HE21; Q59FQ7; Q8WVK6; Q9UF17; A8KAC7; E1CJT9; H0YKU0
UniProt Related Accession #
P48200
GenBank Nucleotide #
NM_004136.2
NCBI Official Full Name
iron-responsive element-binding protein 2
NCBI Official Synonym Full Names
iron responsive element binding protein 2
NCBI Official Symbol
IREB2
NCBI Official Synonym Symbols
ACO3; IRP2; IRP2AD
UniProt Protein Name
Iron-responsive element-binding protein 2
UniProt Synonym Protein Names
Iron regulatory protein 2; IRP2
Protein Family
Iron-responsive element-binding protein
UniProt Gene Name
IREB2
UniProt Synonym Gene Names
IRE-BP 2; IRP2
UniProt Entry Name
IREB2_HUMAN
NCBI Protein Information
iron-responsive element-binding protein 2

UniProt Comments for IREB2

IREB2: RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'- UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. Belongs to the aconitase/IPM isomerase family.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 15q25.1

Cellular Component: cytoplasm

Molecular Function: 4 iron, 4 sulfur cluster binding; iron-responsive element binding; metal ion binding; protein binding; RNA binding; translation repressor activity

Biological Process: cellular iron ion homeostasis; iron ion transport; metabolic process; negative regulation of translation
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Research Articles on IREB2

1. IRP2 can regulate the expression of TfR and Fn by changing its own protein expression and thereby regulate iron metabolism.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IREB2 elisa kit

 Reference Product  PubMed Publications
 MAX elisa kit  >1 publications with IREB2 and MAX

Pathways associated with IREB2 elisa kit

 Products by Pathway  Pathway Diagram
 Iron Metabolism In Placenta Pathway antibodies  Iron Metabolism In Placenta Pathway Diagram
 Validated Targets Of C-MYC Transcriptional Activation Pathway antibodies  Validated Targets Of C-MYC Transcriptional Activation Pathway Diagram

Diseases associated with IREB2 elisa kit

 Disease Name  Pubmed Publications
 Lung Neoplasms Antibodies  >4 publications with IREB2 and Lung Neoplasms
 Nervous System Diseases Antibodies  >4 publications with IREB2 and Nervous System Diseases
 Brain Diseases Antibodies  >4 publications with IREB2 and Brain Diseases
 Anemia Antibodies  >2 publications with IREB2 and Anemia
 Disease Models, Animal Antibodies  >1 publications with IREB2 and Disease Models, Animal
 Hypertension Antibodies  >1 publications with IREB2 and Hypertension
 Liver Diseases Antibodies  >1 publications with IREB2 and Liver Diseases
 Inflammation Antibodies  >1 publications with IREB2 and Inflammation

Organs/Tissues associated with IREB2 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >18 publications with IREB2 and Lung
 Brain Antibodies  >7 publications with IREB2 and Brain
 Blood Antibodies  >5 publications with IREB2 and Blood
 Vascular Antibodies  >2 publications with IREB2 and Vascular
 Muscle Antibodies  >1 publications with IREB2 and Muscle
 Intestine Antibodies  >1 publications with IREB2 and Intestine
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