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Muellerian-Inhibiting Factor (AMH) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS765586

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- AMH antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- AMH antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Muellerian-inhibiting factor (AMH), ELISA Kit
Full Product Name
Human Muellerian-inhibiting factor ELISA Kit
Product Gene Name
AMH elisa kit
Product Synonym Names
AMH/MIS/muellerian-inhibiting factor/Mullerian inhibiting factor//Anti-Muellerian hormone (AMH)/Muellerian-inhibiting substance (MIS)
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
46.875-3000pg/ml
Sensitivity
28.125pg/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of AMH. No significant cross-reactivity or interference between AMH and analogues was observed.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AMH were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AMH were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Molecular Weight
59,195 Da
OMIM
261550
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of AMH elisa kit

Human AMH ELISA Kit – Cat# MBS765586

Other Notes

Small volumes of AMH elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AMH purchase

MBS765586 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Muellerian-inhibiting factor (AMH) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing AMH. The ELISA analytical biochemical technique of the MBS765586 kit is based on AMH antibody-AMH antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect AMH antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, AMH. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for AMH. It may not necessarily be applicable to this product.

NCBI GI #
157266298
NCBI GeneID
268
NCBI Accession #
NP_000470.2
UniProt Secondary Accession #
O75246; Q6GTN3
UniProt Related Accession #
P03971
GenBank Nucleotide #
NM_000479.3
NCBI Official Full Name
muellerian-inhibiting factor
NCBI Official Synonym Full Names
anti-Mullerian hormone
NCBI Official Symbol
AMH
NCBI Official Synonym Symbols
MIF; MIS
UniProt Protein Name
Muellerian-inhibiting factor
UniProt Synonym Protein Names
Anti-Muellerian hormone; AMH; Muellerian-inhibiting substance; MIS
Protein Family
Muellerian-inhibiting factor
UniProt Gene Name
AMH
UniProt Synonym Gene Names
MIF; AMH; MIS
UniProt Entry Name
MIS_HUMAN
NCBI Protein Information
muellerian-inhibiting factor

NCBI Summary for AMH

Anti-Mullerian hormone is a member of the transforming growth factor-beta gene family which mediates male sexual differentiation. Anti-Mullerian hormone causes the regression of Mullerian ducts which would otherwise differentiate into the uterus and fallopian tubes. Some mutations in the anti-Mullerian hormone result in persistent Mullerian duct syndrome. [provided by RefSeq, Jul 2008]
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UniProt Comments for AMH

AMH: This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin. Defects in AMH are the cause of persistent Muellerian duct syndrome type 1 (PMDS1). PMDS1 is a form of male pseudohermaphroditism characterized by a failure of Muellerian duct regression in otherwise normal males. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoplasm; extracellular region; extracellular space

Molecular Function: growth factor activity; hormone activity; receptor binding; transforming growth factor beta receptor binding

Biological Process: activation of NF-kappaB transcription factor; aging; cell-cell signaling; gonadal mesoderm development; Mullerian duct regression; preantral ovarian follicle growth; response to drug; response to organic cyclic substance; sex determination; sex differentiation; urogenital system development

Disease: Persistent Mullerian Duct Syndrome, Types I And Ii
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Research Articles on AMH

1. Dynamic AMH levels may provide a novel intracycle approach to predict response and treatment outcomes after IVF.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with AMH elisa kit

 Reference Product  PubMed Publications
 SOX9 elisa kit  >84 publications with AMH and SOX9
 AMHR2 elisa kit  >48 publications with AMH and AMHR2
 GATA4 elisa kit  >25 publications with AMH and GATA4
 SMAD1 elisa kit  >10 publications with AMH and SMAD1
 BMPR1B elisa kit  >6 publications with AMH and BMPR1B
 SMAD5 elisa kit  >5 publications with AMH and SMAD5
 BMPR1A elisa kit  >4 publications with AMH and BMPR1A
 ACVR1 elisa kit  >2 publications with AMH and ACVR1
 SMAD9 elisa kit  >1 publications with AMH and SMAD9

Pathways associated with AMH elisa kit

 Products by Pathway  Pathway Diagram
 ALK2 Signaling Events Pathway antibodies  ALK2 Signaling Events Pathway Diagram
 BMP Signaling Pathway antibodies  BMP Signaling Pathway Diagram
 BMP Signaling Pathway antibodies  BMP Signaling Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
 CAMP Signaling Pathway antibodies  CAMP Signaling Pathway Diagram

Diseases associated with AMH elisa kit

 Disease Name  Pubmed Publications
 Female Urogenital Diseases Antibodies  >710 publications with AMH and Female Urogenital Diseases
 Genital Diseases, Female Antibodies  >577 publications with AMH and Genital Diseases, Female
 Gonadal Disorders Antibodies  >564 publications with AMH and Gonadal Disorders
 Ovarian Diseases Antibodies  >408 publications with AMH and Ovarian Diseases
 Infertility, Female Antibodies  >371 publications with AMH and Infertility, Female
 Urogenital Abnormalities Antibodies  >179 publications with AMH and Urogenital Abnormalities
 Disorders of Sex Development Antibodies  >157 publications with AMH and Disorders of Sex Development
 Genital Diseases, Male Antibodies  >153 publications with AMH and Genital Diseases, Male
 Infertility, Male Antibodies  >98 publications with AMH and Infertility, Male
 Testicular Diseases Antibodies  >73 publications with AMH and Testicular Diseases

Organs/Tissues associated with AMH elisa kit

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >370 publications with AMH and Testis
 Uterus Antibodies  >75 publications with AMH and Uterus
 Brain Antibodies  >64 publications with AMH and Brain
 Embryonic Tissue Antibodies  >55 publications with AMH and Embryonic Tissue
 Lung Antibodies  >21 publications with AMH and Lung
 Prostate Antibodies  >14 publications with AMH and Prostate
 Intestine Antibodies  >10 publications with AMH and Intestine
 Eye Antibodies  >7 publications with AMH and Eye
 Stomach Antibodies  >2 publications with AMH and Stomach
 Mammary Gland Antibodies  >2 publications with AMH and Mammary Gland
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