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Myoferlin (MYOF) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS765770

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human MYOF antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human MYOF antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Myoferlin, ELISA Kit
Full Product Name
Human Myoferlin ELISA Kit
Product Gene Name
MYOF elisa kit
Product Synonym Names
MYOF/Fer-1-like protein 3
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.625-40ng/ml
Sensitivity
0.375ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
46,695 Da
OMIM
604603
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of MYOF elisa kit

Human MYOF ELISA Kit – Cat# MBS765770

Typical Standard Curve of MYOF elisa kit

Human MYOF ELISA Kit, Typical Standard Curve – Cat# MBS765770

Other Notes

Small volumes of MYOF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MYOF purchase

MBS765770 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Myoferlin, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MYOF. The ELISA analytical biochemical technique of the MBS765770 kit is based on MYOF antibody-MYOF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MYOF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MYOF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MYOF. It may not necessarily be applicable to this product.

NCBI GI #
6731237
NCBI Accession #
AAF27177.1
UniProt Secondary Accession #
Q5VWW2; Q5VWW3; Q5VWW4; Q5VWW5; Q7Z642; Q8IWH0; Q9HBU3; Q9NZM0; Q9ULL3; Q9Y4U4; B3KQN5
NCBI Official Full Name
myoferlin
UniProt Protein Name
Myoferlin
UniProt Synonym Protein Names
Fer-1-like protein 3
Protein Family
Myoferlin
UniProt Gene Name
MYOF
UniProt Synonym Gene Names
FER1L3; KIAA1207
UniProt Entry Name
MYOF_HUMAN

UniProt Comments for MYOF

myoferlin: Calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells that permits rapid resealing of membranes disrupted by mechanical stress. Involved in endocytic recycling. Implicated in VEGF signal transduction by regulating the levels of the receptor KDR. Belongs to the ferlin family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: caveola; cytoplasmic vesicle; cytoplasmic vesicle membrane; integral to membrane; intracellular membrane-bound organelle; nuclear envelope; nuclear membrane; plasma membrane

Molecular Function: phospholipid binding; protein binding

Biological Process: blood circulation; muscle contraction; plasma membrane repair; regulation of vascular endothelial growth factor receptor signaling pathway
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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