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Histone Deacetylase 8 (HDAC8) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS765934

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HDAC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-HDAC antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Histone deacetylase 8 (HDAC8), ELISA Kit
Full Product Name
Human Histone deacetylase 8 ELISA Kit
Product Gene Name
HDAC8 elisa kit
Product Synonym Names
HDAC8 (Histone deacetylase 8)/CDA07/HDACL1/RPD3
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.625-40ng/ml
Sensitivity
0.375ng/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of HDAC. No significant cross-reactivity or interference between HDAC and analogues was observed.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HDAC were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HDAC were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Molecular Weight
16,050 Da
OMIM
300269
3D Structure
ModBase 3D Structure for Q9BY41
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of HDAC8 elisa kit

Human HDAC8 ELISA Kit – Cat# MBS765934

Other Notes

Small volumes of HDAC8 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HDAC8 purchase

MBS765934 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Histone deacetylase 8 (HDAC8) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HDAC8. The ELISA analytical biochemical technique of the MBS765934 kit is based on HDAC8 antibody-HDAC8 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HDAC8 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HDAC8. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HDAC8. It may not necessarily be applicable to this product.

NCBI GI #
7710996
NCBI GeneID
55869
NCBI Accession #
CAB90213.1
UniProt Primary Accession #
Q9BY41
UniProt Secondary Accession #
Q86VC8; Q9NP76; Q9NYH4; A6ND12; A6ND61; A6NET3; A6NJR3; A8MQ62; B4DKN0; B4DV22
UniProt Related Accession #
Q9BY41
NCBI Official Full Name
histone deacetylase 8
NCBI Official Synonym Full Names
histone deacetylase 8
NCBI Official Symbol
HDAC8
NCBI Official Synonym Symbols
HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
UniProt Protein Name
Histone deacetylase 8
Protein Family
Histone deacetylase
UniProt Gene Name
HDAC8
UniProt Synonym Gene Names
HDACL1; HD8
UniProt Entry Name
HDAC8_HUMAN
NCBI Protein Information
histone deacetylase 8

NCBI Summary for HDAC8

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
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UniProt Comments for HDAC8

HDAC8: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. May play a role in smooth muscle cell contractility. Belongs to the histone deacetylase family. HD type 1 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.98; Hydrolase

Chromosomal Location of Human Ortholog: Xq13

Cellular Component: cytoplasm; cytosol; histone deacetylase complex; nuclear chromosome; nucleoplasm; nucleus; plasma membrane

Molecular Function: histone deacetylase activity; Hsp70 protein binding; Hsp90 protein binding; metal ion binding; NAD-dependent histone deacetylase activity (H3-K14 specific); protein binding; transcription factor binding

Biological Process: chromatin assembly or disassembly; chromatin modification; establishment and/or maintenance of chromatin architecture; mitotic cell cycle; negative regulation of protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; regulation of protein stability; regulation of telomere maintenance; sister chromatid cohesion; transcription, DNA-dependent

Disease: Wilson-turner X-linked Mental Retardation Syndrome
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Research Articles on HDAC8

1. Data reveal a role for miR-21-3p in regulating HDAC8 expression and Akt/Gsk3beta pathway in cardiac hypertrophy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HDAC8 elisa kit

 Reference Product  PubMed Publications
 HDAC6 elisa kit  >42 publications with HDAC8 and HDAC6
 HDAC9 elisa kit  >10 publications with HDAC8 and HDAC9
 HDAC10 elisa kit  >9 publications with HDAC8 and HDAC10
 SIN3A elisa kit  >1 publications with HDAC8 and SIN3A

Pathways associated with HDAC8 elisa kit

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 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Chromatin Modifying Enzymes Pathway antibodies  Chromatin Modifying Enzymes Pathway Diagram
 Chromatin Organization Pathway antibodies  Chromatin Organization Pathway Diagram
 Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 HD+PEST Domain Mutants Pathway Diagram
 Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway antibodies  Constitutive Signaling By NOTCH1 PEST Domain Mutants Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram

Diseases associated with HDAC8 elisa kit

 Disease Name  Pubmed Publications
 Neurobehavioral Manifestations Antibodies  >22 publications with HDAC8 and Neurobehavioral Manifestations
 Inflammation Antibodies  >9 publications with HDAC8 and Inflammation
 Chromosome Aberrations Antibodies  >6 publications with HDAC8 and Chromosome Aberrations
 Lung Diseases Antibodies  >5 publications with HDAC8 and Lung Diseases
 Breast Neoplasms Antibodies  >5 publications with HDAC8 and Breast Neoplasms
 Lung Neoplasms Antibodies  >4 publications with HDAC8 and Lung Neoplasms
 Prostatic Neoplasms Antibodies  >3 publications with HDAC8 and Prostatic Neoplasms
 Neoplasms, Experimental Antibodies  >3 publications with HDAC8 and Neoplasms, Experimental
 Hypertrophy Antibodies  >3 publications with HDAC8 and Hypertrophy
 Heart Diseases Antibodies  >3 publications with HDAC8 and Heart Diseases

Organs/Tissues associated with HDAC8 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >15 publications with HDAC8 and Blood
 Muscle Antibodies  >14 publications with HDAC8 and Muscle
 Lung Antibodies  >13 publications with HDAC8 and Lung
 Brain Antibodies  >11 publications with HDAC8 and Brain
 Heart Antibodies  >9 publications with HDAC8 and Heart
 Liver Antibodies  >8 publications with HDAC8 and Liver
 Prostate Antibodies  >8 publications with HDAC8 and Prostate
 Bone Antibodies  >7 publications with HDAC8 and Bone
 Kidney Antibodies  >6 publications with HDAC8 and Kidney
 Nerve Antibodies  >4 publications with HDAC8 and Nerve
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