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Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS765972

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HNRNPF antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-HNRNPF antibody was used as detection antibodies. The standards, test… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Heterogeneous nuclear ribonucleoprotein F (HNRNPF), ELISA Kit
Full Product Name
Human Heterogeneous nuclear ribonucleoprotein F ELISA Kit
Product Gene Name
HNRNPF elisa kit
Product Synonym Names
HNRNPF/Heterogeneous nuclear ribonucleoprotein F/hnRNP F/Nucleolin-like protein mcs94-1/HNRPF/OK/SW-cl.23/mcs94-1
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
78.125-5000pg/ml
Sensitivity
46.875pg/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
45,672 Da
OMIM
601037
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of HNRNPF elisa kit

Human HNRNPF ELISA Kit – Cat# MBS765972

Typical Standard Curve of HNRNPF elisa kit

Human HNRNPF ELISA Kit, Typical Standard Curve – Cat# MBS765972

Other Notes

Small volumes of HNRNPF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HNRNPF purchase

MBS765972 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Heterogeneous nuclear ribonucleoprotein F (HNRNPF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HNRNPF. The ELISA analytical biochemical technique of the MBS765972 kit is based on HNRNPF antibody-HNRNPF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HNRNPF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HNRNPF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HNRNPF. It may not necessarily be applicable to this product.

NCBI GI #
148470406
NCBI GeneID
3185
NCBI Accession #
NP_001091676.1
UniProt Secondary Accession #
Q5T0N2; Q96AU2; B3KM84
UniProt Related Accession #
P52597
GenBank Nucleotide #
NM_001098206.1
NCBI Official Full Name
heterogeneous nuclear ribonucleoprotein F
NCBI Official Synonym Full Names
heterogeneous nuclear ribonucleoprotein F
NCBI Official Symbol
HNRNPF
NCBI Official Synonym Symbols
HNRPF; mcs94-1; OK/SW-cl.23
UniProt Protein Name
Heterogeneous nuclear ribonucleoprotein F
UniProt Synonym Protein Names
Nucleolin-like protein mcs94-1
Protein Family
Heterogeneous nuclear ribonucleoprotein
UniProt Gene Name
HNRNPF
UniProt Synonym Gene Names
HNRPF; hnRNP F
UniProt Entry Name
HNRPF_HUMAN
NCBI Protein Information
heterogeneous nuclear ribonucleoprotein F

NCBI Summary for HNRNPF

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
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UniProt Comments for HNRNPF

hnRNP F: Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state.

Protein type: RNA splicing; Spliceosome; RNA-binding

Chromosomal Location of Human Ortholog: 10q11.21

Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus

Molecular Function: nucleotide binding; protein binding; RNA binding; single-stranded RNA binding; TATA-binding protein binding

Biological Process: gene expression; nuclear mRNA splicing, via spliceosome; regulation of RNA splicing; RNA processing; RNA splicing
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Research Articles on HNRNPF

1. hnRNP F is a co-factor in a subset of tristetraprolin/BRF1/BRF2-mediated mRNA decay.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HNRNPF elisa kit

 Reference Product  PubMed Publications
 HNRNPH1 elisa kit  >1 publications with HNRNPF and HNRNPH1
 HNRNPA2B1 elisa kit  >1 publications with HNRNPF and HNRNPA2B1
 HNRNPK elisa kit  >1 publications with HNRNPF and HNRNPK

Pathways associated with HNRNPF elisa kit

 Products by Pathway  Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies  Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
 MRNA Splicing Pathway antibodies  MRNA Splicing Pathway Diagram
 MRNA Splicing - Major Pathway antibodies  MRNA Splicing - Major Pathway Diagram

Diseases associated with HNRNPF elisa kit

 Disease Name  Pubmed Publications
 Hypertension Antibodies  >2 publications with HNRNPF and Hypertension
 Cardiovascular Diseases Antibodies  >2 publications with HNRNPF and Cardiovascular Diseases
 Fibrosis Antibodies  >2 publications with HNRNPF and Fibrosis
 Hypertrophy Antibodies  >1 publications with HNRNPF and Hypertrophy
 Liver Diseases Antibodies  >1 publications with HNRNPF and Liver Diseases
 Liver Neoplasms Antibodies  >1 publications with HNRNPF and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >1 publications with HNRNPF and Carcinoma, Hepatocellular
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