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Hexokinase-3 (HK3) ELISA Kit for Human

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS765979

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human HK3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HK3 antibody was used as detection antibodies. The… More details

$405 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Hexokinase-3 (HK3), ELISA Kit
Full Product Name
Human Hexokinase-3 ELISA Kit
Product Gene Name
HK3 elisa kit
Product Synonym Names
HK3 (Hexokinase-3)/HK III/Hexokinase type III
Species Reactivity
Human
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Molecular Weight
99,025 Da
OMIM
142570
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Typical Testing Data of HK3 elisa kit

Human HK3 ELISA Kit – Cat# MBS765979

Typical Standard Curve of HK3 elisa kit

Human HK3 ELISA Kit, Typical Standard Curve – Cat# MBS765979

Other Notes

Small volumes of HK3 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HK3 purchase

MBS765979 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hexokinase-3 (HK3) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HK3. The ELISA analytical biochemical technique of the MBS765979 kit is based on HK3 antibody-HK3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HK3 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HK3. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HK3. It may not necessarily be applicable to this product.

NCBI GI #
194097330
NCBI GeneID
3101
NCBI Accession #
NP_002106.2
UniProt Secondary Accession #
Q8N1E7
UniProt Related Accession #
P52790
GenBank Nucleotide #
NM_002115.2
NCBI Official Full Name
hexokinase-3
NCBI Official Synonym Full Names
hexokinase 3
NCBI Official Symbol
HK3
NCBI Official Synonym Symbols
HXK3; HKIII
UniProt Protein Name
Hexokinase-3
UniProt Synonym Protein Names
Hexokinase type III; HK III
Protein Family
Probable histidine kinase
UniProt Gene Name
HK3
UniProt Synonym Gene Names
HK III
UniProt Entry Name
HXK3_HUMAN
NCBI Protein Information
hexokinase-3

NCBI Summary for HK3

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq, Apr 2009]

UniProt Comments for HK3

HK3: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Protein type: Enzyme, cellular metabolism; Carbohydrate Metabolism - starch and sucrose; Kinase, other; Carbohydrate Metabolism - fructose and mannose; Transferase; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 2.7.1.1; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - glycolysis and gluconeogenesis

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: cytosol; mitochondrion

Molecular Function: ATP binding; fructokinase activity; glucokinase activity; glucose binding; hexokinase activity; mannokinase activity

Biological Process: carbohydrate metabolic process; carbohydrate phosphorylation; cell glucose homeostasis; glucose 6-phosphate metabolic process; glucose metabolic process; glucose transport; glycolysis; hexose transport; transmembrane transport
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Research Articles on HK3

1. Through glycolysis and arachidonic acid metabolism, HK3 and PTGS2 might play important roles in pediatric ALL and its prognosis, and thus, might be potential targets for therapeutic intervention to suppress pediatric ALL.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HK3 elisa kit

 Reference Product  PubMed Publications
 PFKFB2 elisa kit  >1 publications with HK3 and PFKFB2

Pathways associated with HK3 elisa kit

 Products by Pathway  Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Butirosin And Neomycin Biosynthesis Pathway antibodies  Butirosin And Neomycin Biosynthesis Pathway Diagram
 Butirosin And Neomycin Biosynthesis Pathway antibodies  Butirosin And Neomycin Biosynthesis Pathway Diagram
 Carbohydrate Digestion And Absorption Pathway antibodies  Carbohydrate Digestion And Absorption Pathway Diagram
 Carbohydrate Digestion And Absorption Pathway antibodies  Carbohydrate Digestion And Absorption Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram

Diseases associated with HK3 elisa kit

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >10 publications with HK3 and Carcinoma
 Hyperplasia Antibodies  >8 publications with HK3 and Hyperplasia
 Neoplasm Metastasis Antibodies  >4 publications with HK3 and Neoplasm Metastasis
 Carcinoma, Hepatocellular Antibodies  >2 publications with HK3 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >2 publications with HK3 and Liver Neoplasms
 Liver Diseases Antibodies  >2 publications with HK3 and Liver Diseases
 Neoplasms, Experimental Antibodies  >1 publications with HK3 and Neoplasms, Experimental
 Cell Transformation, Neoplastic Antibodies  >1 publications with HK3 and Cell Transformation, Neoplastic
 Lung Neoplasms Antibodies  >1 publications with HK3 and Lung Neoplasms
 Inflammation Antibodies  >1 publications with HK3 and Inflammation

Organs/Tissues associated with HK3 elisa kit

 Organ/Tissue Name  Pubmed Publications
 Prostate Antibodies  >54 publications with HK3 and Prostate
 Blood Antibodies  >24 publications with HK3 and Blood
 Brain Antibodies  >5 publications with HK3 and Brain
 Skin Antibodies  >5 publications with HK3 and Skin
 Testis Antibodies  >5 publications with HK3 and Testis
 Lung Antibodies  >3 publications with HK3 and Lung
 Muscle Antibodies  >2 publications with HK3 and Muscle
 Connective Tissue Antibodies  >1 publications with HK3 and Connective Tissue
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